Genomospecies identification and phylogenomic relevance of AFLP analysis of isolated and non-isolated strains of Frankia spp.

Bautista, Guadalupe; Cruz, H.A.; Nesme, Xavier; Valdés, Marı́a; Mendoza, Heidi; Fernández, María P.

doi:10.1016/j.syapm.2010.10.004

Cited by 13 publications

(11 citation statements)

References 44 publications

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“…Clade 1 was close to F. alni reference strains (clade 2, G1) as suggested in previous studies (Nazaret et al ., ; Normand et al ., ; Bautista et al ., ). The genetic distance between clade 1 and clade 2 did not support the exclusion of clade 1 from G1.…”

Section: Discussionmentioning

confidence: 97%

“…A first evidence of genetic difference between Sp+ and Sp− strains was given by determining ribosomal oligonucleotide primers specific to each type of strains (Simonet et al, 1994). Besides, Sp+ Alnus strains were positioned close to Frankia alni (G1) in Frankia phylogenies (Nazaret et al, 1991;Normand et al, 1996;Bautista et al, 2011). However, these studies often based on a unique or few nodules or sites did not allow describing Sp+ diversity and phylogeny.…”

Section: Discussionmentioning

confidence: 99%

“…(**) Clusters 1 to 4: Normand et al, 1996. (***) (A; An et al, 1985), (B; Baker and Torrey, 1980), (C; Bautista et al, 2011), (D; Bautista-Guerrero H, Abby S, Herrera-Belaroussi A, Valdés M, Fernandez MP, unpublished), (E; Bloom et al, 1989), (F; Clawson and Benson, 1999), (G;Fernandez et al, 1989), (H; Normand and Lalonde, 1986), (I; Lalonde et al, 1981), (J; Moiroud and Guillaumaud, unpublished), (K; Persson et al, 2011), (L;Zhang et al, 1984). Table S2.…”

Section: Fig S1mentioning

confidence: 99%

“…Phylogenetic studies on Frankia seldom included Sp+ endophytes. Only Alnus viridis Sp+ endophytes were characterized and positioned close to cluster 1 but the very few number of specimens analysed did not allow generalization about Sp+ phylogeny and diversity (Nazaret et al, 1991;Normand et al, 1996;Bautista et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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In‐planta sporulation phenotype: a major life history trait to understand the evolution of Alnus‐infective Frankia strains

Pozzi

Bautista-Guerrero

Nouioui

et al. 2014

Environmental Microbiology

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Two major types of Frankia strains are usually recognized, based on the ability to sporulate in-planta: spore-positive (Sp+) and spore-negative (Sp-). We carried out a study of Sp+ and Sp- Frankia strains based on nodules collected on Alnus glutinosa, Alnus incana and Alnus viridis. The nodules were phenotyped using improved histology methods, and endophytic Frankia strain genotype was determined using a multilocus sequence analysis approach. An additional sampling was done to assess the relation between Sp+ phenotype frequency and genetic diversity of Frankia strains at the alder stand scale. Our results revealed that (i) Sp+ and Sp- Alnus-infective Frankia strains are genetically different even when sampled from the same alder stand and the same host-plant species; (ii) there are at least two distinct phylogenetic lineages of Sp+ Frankia that cluster according to the host-plant species and without regard of geographic distance and (iii) genetic diversity of Sp+ strains is very low at the alder stand scale compared with Sp- strains. Difference in evolutionary history and genetic diversity between Sp+ and Sp- Frankia allows us to discuss the possible ecological role of in-planta sporulation.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Fig S1mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In‐planta sporulation phenotype: a major life history trait to understand the evolution of Alnus‐infective Frankia strains

Pozzi

Bautista-Guerrero

Nouioui

et al. 2014

Environmental Microbiology

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“…Some concordance occurs between these described genospecies and the subsequent phenotypic clustering proposed by Dobritsa (1998) and may reflect the taxonomic structure of the genus Frankia. Further data or analysis is required to support this model; for instance, amplified fragment length polymorphism (AFLP) not only provides a resolution equivalent to DNA-DNA hybridization but has two added advantages: (1) it permits inferring phylogenetic relationships between genospecies (Coenye et al 1999;Rademaker et al 2000;Portier et al 2006) and (2) is useful in assigning uncultured Frankia strains present in nodules containing a mixture of the microbe Nazaret et al (1989Nazaret et al ( , 1991 (continued) et al (2004) and the plant DNA (Bautista et al 2011). The nine genospecies as defined by Fernandez et al (1989) have been validated and supplementary strains have been assigned to genospecies (Table 7.3) (Bautista et al 2011).…”

Section: Frankia Genetic Diversitymentioning

confidence: 99%

Diversity of Frankia Strains, Actinobacterial Symbionts of Actinorhizal Plants

2013

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In the last edition of the Bergey's Manual of Systematic Bacteriology, Frankia was the sole genus found in the family Frankiaceae. This single-genus family, together with Geodermatophilaceae, Nakamurellaceae, Sporichthyaceae, Acidothermaceae, and Cryptosporangiaceae, makes up the order Frankiales, an artificial taxon within the phylum Actinobacteria supported only marginally by 16S rDNA similarities (Normand and Benson 2012). Genus Frankia represents a monophyletic assemblage of soil actinobacteria distinguishable by multilocular sporangia-forming branched hyphae and their unique ability to grow in nitrogen-limited substrates due to their ability to fix nitrogen within specialized thick-walled structures, termed vesicles, produced in vitro and in planta. Diversity of Frankia has been noted in several separate reports on the presence of comparable root nodule structures induced in numerous trees and shrubs growing as aggressive pioneers in nitrogenlimited ecosystems such as glacial moraines, gravel slopes, volcanic ashes, mine spoils, or burned forests with a contributed input of 15 % of total biologically fixed nitrogen on earth (Quispel 1990). Despite the absence of apparent close kinship among these plants, they have been termed actinorhizal plants due to the nature of the root nodule-causing agents detected in plant tissues. Based on in planta morphology and the limited cross-infectivity of crushed-nodule inocula, Becking

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Frankia

Normand¹,

Fernández²

2019

Bergey's Manual of Systematics of Archaea and Bacteria

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Fran.ki.a. N.L. fem. n. Frankia named after Albert Bernhard Frank (1839–1900), a Swiss plant biologist, who studied extensively nitrogen nutrition in legumes and the microorganisms causing root nodulation from 1877 to 1892 and who coined the term “symbiosis.” Actinobacteria / Actinobacteria / Frankiales / Frankiaceae / Frankia A group of soil‐inhabiting mesophilic, mostly nitrogen‐fixing, organisms in symbiosis with pioneer plants collectively called “actinorhizal plants” in orders Cucurbitales, Fagales, and Rosales. Frankia isolates have branched septate hyphae, multilocular sporangia, and for the most part vesicles or diazo‐vesicles, specialized cells where nitrogen fixation takes place. All Frankia strains sporulate in culture, but only some genotypes maintain the capacity to form abundant sporangia within plant cells. Around 50% of endophytic Frankia have resisted isolation. Growth is slow, and it took a long time to obtain isolates after decades of unsuccessful attempts. Isolates are mostly white, sometimes pigmented, have mostly simple growth requirements, and can fix nitrogen in pure culture or in symbiotic nodules except for a small number of nonsymbiotic isolates. The genus comprises 11 species and 2 candidatus grouped into four phylogenetic clusters at the root of aerobic actinobacteria. DNA G + C content (mol % ) : 67.9–72.8. Type species : Frankia alni (Woronin 1866) Von Tubeuf 1895 AL .

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Genomospecies identification and phylogenomic relevance of AFLP analysis of isolated and non-isolated strains of Frankia spp.

Cited by 13 publications

References 44 publications

In‐planta sporulation phenotype: a major life history trait to understand the evolution of Alnus‐infective Frankia strains

In‐planta sporulation phenotype: a major life history trait to understand the evolution of Alnus‐infective Frankia strains

Diversity of Frankia Strains, Actinobacterial Symbionts of Actinorhizal Plants

Frankia

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