Genomics of Protein Secretion and Hyphal Growth in Aspergillus

Abstract

a These sites provide an entry point to the sequence data but access requires agreement being reached with the respective company b Genome sequence data for A. oryzae are not yet available. A summary of the A. oryzae genome sequencing project and related topics has been published (Machida 2002) Table. 5.1. Websites for Aspergillus genome information (modified after Archer and Dyer 2004) Table. 5.2. Summary of information derived from the genome sequences of Aspergillus species a A. fumigatus b A. nidulans c A. niger d A. oryzae e Genome size (Mb) f 29 30 36 37 Predicted genes f 10,000 10,000 14,000 14,000 Genes with Pfam hits f 4,400 4,500 5,300 5,300 a This table was published in extended form elsewhere (Archer and Dyer 2004), and the data were obtained from the websites and contributions from William Nierman (TIGR, USA), James Galagan (Broad Institute, USA), Masa Machida (Tsukuba, Japan), and Gert Groot and Noel van Peij (DSM, The Netherlands) b

Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

“…Aspergillus enzymes are used in starch processing, baking, brewing and beverage industries, in animal feed and in the paper and pulping industry. Furthermore, A. niger is used as host for the production of heterologous proteins 4,5 and as cell factory for the production of citric acid and gluconic acid 6 . A. niger exhibits a remarkably versatile metabolism, enabling growth on a wide range of substrates under various environmental conditions.…”

Transcriptomic Insights into the Physiology of Aspergillus niger Approaching a Specific Growth Rate of Zero

“…The perspective is that A. niger cultures as they approach a zero growth rate can be used as a cell factory for production of secondary metabolites and cysteine-rich proteins. We propose that the improved retentostat method can be used in fundamental studies of differentiation and is applicable to filamentous fungi in general.The filamentous fungus Aspergillus niger is one of the most important microorganisms in industrial biotechnology because of its ability to produce high levels of enzymes and organic acids (6,50,57). Apart from their biotechnological importance, filamentous fungi in the Aspergillus section Nigri (the black aspergilli) represent some of the most widespread storage molds which contaminate food and feedstocks with mycotoxins (26,37,48).…”

“…The filamentous fungus Aspergillus niger is one of the most important microorganisms in industrial biotechnology because of its ability to produce high levels of enzymes and organic acids (6,50,57). Apart from their biotechnological importance, filamentous fungi in the Aspergillus section Nigri (the black aspergilli) represent some of the most widespread storage molds which contaminate food and feedstocks with mycotoxins (26,37,48).…”

Genomics of protein folding in the endoplasmic reticulum, secretion stress and glycosylation in the aspergilli

“…These topics have been reviewed extensively and repeatedly in higher and lower eukaryotes. A recent review from Schröder (2008a) summarizes the molecular basis of ER stress in higher eukaryotes and other reviews focus on S. cerevisiae (Schröder and Kaufman, 2005) or the filamentous fungi (Archer and Turner, 2006). The UPR has been implicated in several cellular processes, including the regulation of expression of the ER lumenal environment (e.g.…”

“…For more detailed review of the chaperone cycle and the foldase activity, refer to Schröder (2008a,b). therefore, the UPR has often been viewed as an area that could be targeted for manipulation in order to provide improved strains for production purposes (Schröder, 2008b;Archer and Turner, 2006). S. cerevisiae can be used as the organism against which the aspergilli should be compared at the genomic level because of its status as a well-studied model fungus.…”