Genomics of Invasive Cutibacterium acnes Isolates from Deep-Seated Infections

Both, Anna; Huang, Jia-Bin; Hentschke, Moritz; Tobys, David; Martin, Coralie; Seifert, Harald; Aepfelbacher, Martin; Rohde, Holger

doi:10.1128/spectrum.04740-22

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…All six pathogen helper C. acnes strains did not show any skin symptoms when intradermally infected alone. The lack of pathogenesis in pathogen helper C. acnes was also supported by MLST analysis, demonstrating that among the six C. acnes strains, five consisted of the IB type, which has been previously reported as the predominant commensal phylogroup 50 – 52 . Our data additionally revealed that C. acnes can not only promote S. aureus growth but also worsen symptoms of skin infection.…”

Section: Discussionsupporting

confidence: 66%

Facemask acne attenuation through modulation of indirect microbiome interactions

Na,

Kim,

Kim

et al. 2024

npj Biofilms Microbiomes

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During the COVID-19 pandemic, facemasks played a pivotal role in preventing person-person droplet transmission of viral particles. However, prolonged facemask wearing causes skin irritations colloquially referred to as ‘maskne’ (mask + acne), which manifests as acne and contact dermatitis and is mostly caused by pathogenic skin microbes. Previous studies revealed that the putative causal microbes were anaerobic bacteria, but the pathogenesis of facemask-associated skin conditions remains poorly defined. We therefore characterized the role of the facemask-associated skin microbiota in the development of maskne using culture-dependent and -independent methodologies. Metagenomic analysis revealed that the majority of the facemask microbiota were anaerobic bacteria that originated from the skin rather than saliva. Previous work demonstrated direct interaction between pathogenic bacteria and antagonistic strains in the microbiome. We expanded this analysis to include indirect interaction between pathogenic bacteria and other indigenous bacteria classified as either ‘pathogen helper (PH)’ or ‘pathogen inhibitor (PIn)’ strains. In vitro screening of bacteria isolated from facemasks identified both strains that antagonized and promoted pathogen growth. These data were validated using a mouse skin infection model, where we observed attenuation of symptoms following pathogen infection. Moreover, the inhibitor of pathogen helper (IPH) strain, which did not directly attenuate pathogen growth in vitro and in vivo, functioned to suppress symptom development and pathogen growth indirectly through PH inhibitory antibacterial products such as phenyl lactic acid. Taken together, our study is the first to define a mechanism by which indirect microbiota interactions under facemasks can control symptoms of maskne by suppressing a skin pathogen.

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Section: Discussionsupporting

confidence: 66%

Facemask acne attenuation through modulation of indirect microbiome interactions

Na,

Kim,

Kim

et al. 2024

npj Biofilms Microbiomes

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“…C. acnes is generally composed of several phylogenetic subtypes, commonly referred to as IA 1 , IA 2 , IB, IC, II, III 17 . At the genome level, even finer classifications are possible 17,39 . In this study, a genetic analysis was conducted to determine the phylotype ratio of C. acnes in all subjects.…”

Section: Discussionmentioning

confidence: 99%

Detailed microbiome analysis of sticker-stripped surface materials of acne lesions revealed acne-relatedCutibacterium acnessubtypes: a pilot study

Shimokawa,

Funatsu,

Ohata

et al. 2023

Preprint

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Cutibacterium acnes (C. acnes)is known to play a central role in pathogenesis of acne vulgaris. It has been understood that multiple phylotypes ofC. acnesexist, with certain types being more prevalent in patient with acne vulgaris and others more common in healthy individuals. In this context, we conducted a preliminary study using self-collected samples via an adhesive sticker (MySkin®) to analyze the skin microbiome of Japanese women. The study aimed to determine the role ofC. acnesand its specific phylotypes in the development of acne vulgaris.Participants in this study were Japanese females aged between their 20s and 40s. Dermatologists evaluate the data from web-based questionnaires and smartphone image submissions to classify subjects into either the Acne or Non-acne group. Quality assessment of DNA extracted from the sticker was conducted, followed by amplification of the16S rRNAregion using PCR. Subsequent microbial community analysis was performed using next-generation sequencing techniques. Genetic classification ofC. acneswas accomplished through single locus sequence typing.Results indicated a bacterial community composition on the facial skin surface predominantly consisting ofC. acnesclusters, with over half of these clusters constituted byC. acnes. Notably, the Acne group exhibited a significantly higher proportion ofC. acnesrelative to total bacterial presence compared to the Non-acne group. Analysis ofC. acnesphylotypes revealed a markedly lower presence of type III in the Acne group (vs. Non-acne group,p<0.05). No significant differences were observed in the prevalence of Types IA1, IA2, II, and IB between the two groups. The predominant phylotypes ofC. acnesidentified were IA2_2_F0 (23.9%), IA1_4_A0 (20.6%), and II_2_K0 (18.6%). Within the Acne group, an increase in IA2_1_F1 and a decrease in III_1_L0 were observed (vs. Non-acne group, p<0.05).This study underscores the feasibility of using self-collected and mailed-in samples for qPCR and microbiome analysis, maintaining diagnostic quality comparable to in-person assessments. Furthermore, the variation in the expression ofC. acnesphylotypes across skin surfaces between acne-afflicted and healthy individuals could suggest that shifts in phylotype expression patterns may be indicative of skin susceptibilities to acne development.

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2023 Neer Award for Basic Science: Genetics of Cutibacterium acnes in revision shoulder arthroplasty: a large-scale bacterial whole-genome sequencing study

Hsu,

Matsen,

Whitson

et al. 2024

Journal of Shoulder and Elbow Surgery

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Genomics of Invasive Cutibacterium acnes Isolates from Deep-Seated Infections

Cited by 4 publications

References 51 publications

Facemask acne attenuation through modulation of indirect microbiome interactions

Facemask acne attenuation through modulation of indirect microbiome interactions

Detailed microbiome analysis of sticker-stripped surface materials of acne lesions revealed acne-relatedCutibacterium acnessubtypes: a pilot study

2023 Neer Award for Basic Science: Genetics of Cutibacterium acnes in revision shoulder arthroplasty: a large-scale bacterial whole-genome sequencing study

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