Genomics and proteomics in stem cell research: the road ahead

Ahn, Sung‐Min; Simpson, Richard J.; Lee, Bong-Hee

doi:10.5115/acb.2010.43.1.1

Cited by 13 publications

(5 citation statements)

References 124 publications

(139 reference statements)

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“…Embryonic and non-embryonic stem cells have been considered as potential therapeutic approaches for several types of diseases. One type of adult stem cell, the mesenchymal stem cell (MSC), has attracted a great deal of attention in the field of regenerative medicine due to its unique biological properties [1]. Bone marrow-derived MSCs (BMSCs) were first identified in 1968 by Friedenstein as an adherent fibroblast-like cell in the bone marrow that was capable of differentiating into bone [2].…”

Section: Introductionmentioning

confidence: 99%

Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study

et al. 2013

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In recent years, examination and comparison of the biological characteristics of bone marrow- and adipose-derived mesenchymal stem cells (MSCs) from various perspectives have come into the focus of stem cell research, as these cells should be well characterized in order to utilize them in future cellular therapies. Therefore, in the present study, surface protein markers and the skeletal myogenic differentiation potential of rat bone marrow- and adipose-derived MSCs were examined. The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry. Subsequently, the stem cells were differentiated into myogenic lineages, and the expression of the skeletal myogenic markers MyoD1, Myog, and Myh2 was studied in cells using real time polymerase chain reaction and immunofluorescence. Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential. Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.

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Section: Introductionmentioning

confidence: 99%

Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study

et al. 2013

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“…Human embryonic stem cells (hESCs) provide valuable insight into human development and disease, have exciting potential in regenerative medicine, and can be used as models for drug discovery and toxicity analyses (reviewed in 1). These cells are derived from the inner cell mass of a pre‐implantation embryo, called the blastocyst 2.…”

Section: Introductionmentioning

confidence: 99%

Proteomics of human embryonic stem cells

et al. 2011

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Human embryonic stem cells (hESCs) offer exciting potential in regenerative medicine for the treatment of a host of diseases including cancer, Alzheimer's and Parkinson's disease. They also provide insight into human development and disease and can be used as models for drug discovery and toxicity analyses. The key properties of hESCs that make them so promising for medical use are that they have the ability to self-renew indefinitely in culture and they are pluripotent, which means that they can differentiate into any of more than 200 human cell types. Since proteins are the effectors of cellular processes, it is important to investigate hESC expression at the protein level as well as at the transcript level. In addition, post-translational modifications, such as phosphorylation, may influence the activity of pivotal proteins in hESCs, and this information can only be determined by studying the proteome. In this review, we summarize the results obtained from several proteomics analyses of hESCs that have been reported in the last few years.

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“…Increasing attention is paid to enrich and characterize membrane and plasma‐membrane proteins of PSCs in search of surface markers that can define stem cells, and their developmental stages . Previous success on identifying the “cluster of differentiation” (CD) markers in leukocytes coming out of the Human Leukocyte Differentiation Antigens Workshop (HLDA) encouraged the surface proteomics of PSCs. Many techniques have been explored to characterize membrane proteomes of PSCs as summarized in a few reviews in 2008 .…”

Section: Functional Importance Of Cell Surface Proteinsmentioning

confidence: 99%

Proteomics and glycoproteomics of pluripotent stem‐cell surface proteins

Sun

2014

Proteomics

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Pluripotent stem cells are a unique cell type with promising potential in regenerative and personalized medicine. Yet the difficulty to understand and coax their seemingly stochastic differentiation and spontaneous self-renewal have largely limited their clinical applications. A call has been made by numerous researchers for a better characterization of surface proteins on these cells, in search of biomarkers that can dictate developmental stages and lineage specifications, and can help formulate mechanistic insight of stem-cell fate choices. In the past two decades, proteomics has gained significant recognition in profiling surface proteins at high throughput. This review will summarize the impact of these studies on stem-cell biology, and discuss the used proteomic techniques. A systematic comparison of all the techniques and their results is also attempted here to help reveal pros, cons, and the complementarity of the existing methods. This awareness should assist in selecting suitable strategies for stem-cell related research, and shed light on technical improvements that can be explored in the future.

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Genomics and proteomics in stem cell research: the road ahead

Cited by 13 publications

References 124 publications

Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study

Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study

Proteomics of human embryonic stem cells

Proteomics and glycoproteomics of pluripotent stem‐cell surface proteins

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