Genomics-Aided Breeding for Climate-Smart Traits in Faba Bean

Sallam, Ahmed; Ul‐Allah, Sami

doi:10.1007/978-3-319-96932-9_7

Cited by 5 publications

(6 citation statements)

References 99 publications

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“…All SNPs detected in the two aforementioned studies were located on chromosomes 1, 2, 3, 4, and 6 in the FBCM (Webb et al, 2016). Most of the SNPs associated with frost tolerance in the shoots were located on chromosome 1 (Sallam and Ul-Allah, 2019). In the present study, the nine identified SNPs were located on chromosomes 1, 3, 5, and 6.…”

Section: Genome-wide Associationsupporting

confidence: 54%

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Genome-wide association mapping for root traits associated with frost tolerance in faba beans using KASP-SNP markers

et al. 2022

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Frost is an abiotic stress factor that threatens plant development and crop productivity not only in cold regions but also in temperate zones. Roots play an important role in plant growth during frost stress. Therefore, variation in root characteristics could be studied to improve frost tolerance in winter faba bean. The present study aimed to identify the genomic regions that control frost tolerance in a winter faba bean population by focusing on root-related traits. A set of 185 genotypes were tested for frost tolerance under artificial frost growth conditions at −16°C, −18°C, and −19°C in a growth chamber. Frost stress reduced the root-related parameters in all genotypes, with a wide variation among genotypes. A genome-wide association study identified nine novel single-nucleotide polymorphisms that are associated with the root-related traits. The most frost-tolerant genotypes were identified; two genotypes, S_028 and S_220, exhibited remarkable performance under frost stress. Moreover, they harbored all four of the alleles favorable for frost tolerance. Remarkably, two markers showed genetic pleiotropic effects with positive allele effects on root fresh matter and root dry matter. Thus, both genotypes can be implemented in a breeding program to provide the alleles for healthier roots under frost conditions to develop more frost-tolerant varieties, and the two markers can be used to screen large collections to select for frost tolerance. These results may provide novel insights for improving frost tolerance in faba beans and in other legume crops.

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Section: Genome-wide Associationsupporting

confidence: 54%

“…Thus, genotyping by sequencing in faba beans remains a challenging task. The KASP markers used in the present study may be the best option for genetic studies to identify markers associated with target traits in faba beans (Sallam and Ul-Allah, 2019). This set of KASP markers was initially developed by Webb et al (2016).…”

Section: Genome-wide Associationmentioning

confidence: 99%

Genome-wide association mapping for root traits associated with frost tolerance in faba beans using KASP-SNP markers

et al. 2022

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“…Some progress has been made in identifying QTLs for abiotic stresses such as frost tolerance (Arbaoui et al, 2008 ; Sallam et al, 2016 ; Sallam & Martsch, 2015 ), traits related to drought adaptation (Ali et al, 2016 ; Khazaei et al, 2014a ), and yield (Ávila et al, 2017 ; Cruz‐Izquierdo et al, 2012 ). QTLs controlling abiotic stress responses in faba bean, detected by either QTL mapping or association mapping approaches, have been discussed by Sallam and Ul‐Allah ( 2019 ). Given the few QTLs reported in faba bean compared with other pulses, saturation of the genomic regions associated with target regions and QTL validation in multiple environments and genetic backgrounds are needed to uncover reliable marker‐trait associations such as those reported by Aguilar‐Benitez et al ( 2020 ) for pod dehiscence.…”

Section: Genomic Resourcesmentioning

confidence: 99%

Recent advances in faba bean genetic and genomic tools for crop improvement

et al. 2021

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Faba bean (Vicia faba L.), a member of the Fabaceae family, is one of the important food legumes cultivated in cool temperate regions. It holds great importance for human consumption and livestock feed because of its high protein content, dietary fibre, and nutritional value. Major faba bean breeding challenges include its mixed breeding system, unknown wild progenitor, and genome size of ~13 Gb, which is the largest among diploid field crops. The key breeding objectives in faba bean include improved resistance to biotic and abiotic stress and enhanced seed quality traits. Regarding quality traits, major progress on reduction of vicine‐convicine and seed coat tannins, the main anti‐nutritional factors limiting faba bean seed usage, have been recently achieved through gene discovery. Genomic resources are relatively less advanced compared with other grain legume species, but significant improvements are underway due to a recent increase in research activities. A number of bi‐parental populations have been constructed and mapped for targeted traits in the last decade. Faba bean now benefits from saturated synteny‐based genetic maps, along with next‐generation sequencing and high‐throughput genotyping technologies that are paving the way for marker‐assisted selection. Developing a reference genome, and ultimately a pan‐genome, will provide a foundational resource for molecular breeding. In this review, we cover the recent development and deployment of genomic tools for faba bean breeding.

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“…In order to compare the positions of frost tolerance-related QTLs from this study with those described earlier in faba bean, and in absence of a reference genome sequence for this crop, we took advantage of the bridge markers between the map published by Webb et al. (2016 ) and our map ( Carrillo-Perdomo et al, 2020 ) as Sallam and Ul-Allah (2019) report QTLs and association peaks anchored to the Webb et al. map.…”

Section: Methodsmentioning

confidence: 99%

A QTL approach in faba bean highlights the conservation of genetic control of frost tolerance among legume species

Carrillo‐Perdomo

Magnin-Robert²,

Raffiot³

et al. 2022

Front. Plant Sci.

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Frost is a major abiotic stress of winter type faba beans (Vica faba L.) and has adverse effects on crop yield. Climate change, far from reducing the incidence of frost events, is making these phenomena more and more common, severe, and prolonged. Despite the important interaction that the environment has in the tolerance of faba bean to frost, this trait seems to have good levels of heritability. Several QTLs for frost tolerance have already been reported, however, a more robust identification is needed to more precisely identify the genomic regions involved in faba bean tolerance to sub-zero temperatures. Several pea (Pisum sativum L.) and barrel medic (Medicago truncatula L.) frost tolerance QTLs appear to be conserved between these two species, furthering the hypothesis that the genetic control of frost tolerance in legume species might be more generally conserved. In this work, the QTL mapping in two faba bean recombinant inbred line (RIL) populations connected by a common winter-type parent has led to the identification of five genomic regions involved in the control of frost tolerance on linkage groups I, III, IV, and V. Among them, a major and robust QTL of great interest for marker-assisted selection was identified on the lower part of the long-arm of LGI. The synteny between the faba bean frost tolerance QTLs and those previously identified in other legume species such as barrel medic, pea or soybean highlighted at least partial conservation of the genetic control of frost tolerance among different faba bean genetic pools and legume species. Four novel RILs showing high and stable levels of tolerance and the ability to recover from freezing temperatures by accumulating frost tolerance QTLs are now available for breeding programs.

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Genomics-Aided Breeding for Climate-Smart Traits in Faba Bean

Cited by 5 publications

References 99 publications

Genome-wide association mapping for root traits associated with frost tolerance in faba beans using KASP-SNP markers

Genome-wide association mapping for root traits associated with frost tolerance in faba beans using KASP-SNP markers

Recent advances in faba bean genetic and genomic tools for crop improvement

A QTL approach in faba bean highlights the conservation of genetic control of frost tolerance among legume species

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