Genomic Variation between Genetic Lines of White Leghorns Differed in Resistance to Marek’s Disease

Xie, Qingmei; Chang, Shuang; Dong, Kunzhe; Dunn, John R.; Song, Jiuzhou; Zhang, Huanmin

doi:10.21767/2472-1158.100063

Cited by 7 publications

(10 citation statements)

References 48 publications

(83 reference statements)

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“…1.37E−4 < FDR < 1.31E−2) and 26 were downregulated (log 2 FC: −11.45 to −1.24; 6.58E−47 < p < 1.89E−3; 5.09E−44 < FDR < 4.06E−2) in line 6 3 birds in contrast to line 7 2 in response to HVT inoculation. Thirty identified miRNAs (6 known-miRNAs and 24 novel miR-NAs) were differentially expressed between line 6 3 (Table 3).…”

Section: Differentially Expressed Micrornas In Response To Hvt or CVImentioning

confidence: 99%

“…MD was first described by József Marek, a Hungarian veterinarian [1]. Clinical symptoms of MD vary among different lines of chickens and are dependent of exposure to different strains of MDV [2,3], but commonly include polyneuritis, visceral lymphoma, acute transient paralysis, immunosuppression, brain edema, and acute rash [4,5]. The morbidity and mortality could range from 11 [6] up to 100% [3].…”

Section: Introductionmentioning

confidence: 99%

“…Clinical symptoms of MD vary among different lines of chickens and are dependent of exposure to different strains of MDV [2,3], but commonly include polyneuritis, visceral lymphoma, acute transient paralysis, immunosuppression, brain edema, and acute rash [4,5]. The morbidity and mortality could range from 11 [6] up to 100% [3]. Although MD has been well under control most of the time in most regions of the world by wide use of MD vaccines and improved management measures since the 1970s [7][8][9], sporadic outbreaks still occur from time to time all over the world.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to B-haplotypes, non-MHC host genetic components also play a significant role modulating MD vaccine efficacy against MD [24]. The two highly inbred genetic lines of chickens, lines 6 3 and 7 2 , maintained at USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory (East Lansing, Michigan, USA), share a common B*2 haplotype, but line 6 3 is relatively resistant and line 7 2 is highly susceptible to MD [3]. Data from vaccination and challenge trials conducted in recent years showed that line 6 3 chickens can convey better or equivalent protective efficacy in response to MDV-3 HVT vaccination than MDV-1 CVI988/Rispens.…”

Section: Introductionmentioning

confidence: 99%

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Marek’s disease vaccines-induced differential expression of known and novel microRNAs in primary lymphoid organ bursae of White Leghorn

Zhang

Zhu

Heidari

et al. 2020

Vet Res

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Marek's disease (MD) is a contagious disease of domestic chickens caused by MD viruses. MD has been controlled primarily by vaccinations, yet sporadic outbreaks of MD take place worldwide. Commonly used MD vaccines include HVT, SB-1 and CVI988/Rispens and their efficacies are reportedly dependent of multiple factors including host genetics. Our previous studies showed protective efficacy of a MD vaccine can differ drastically from one chicken line to the next. Advanced understanding on the underlying genetic and epigenetic factors that modulate vaccine efficacy would greatly improve the strategy in design and development of more potent vaccines. Two highly inbred lines of White Leghorn were inoculated with HVT and CVI988/Rispens. Bursa samples were taken 26 days post-vaccination and subjected to small RNA sequencing analysis to profile microRNAs (miRNA). A total of 589 and 519 miRNAs was identified in one line, known as line 6 3 , 490 and 630 miRNAs were identified in the other, known as line 7 2 , in response to HVT or CVI988/Rispens inoculation, respectively. HVT and CVI988/Rispens induced mutually exclusive 4 and 13 differentially expressed (DE) miRNAs in line 6 3 birds in contrast to a non-vaccinated group of the same line. HVT failed to induce any DE miRNA and CVI988/Rispens induced a single DE miRNA in line 7 2 birds. Thousands of target genes for the DE miRNAs were predicted, which were enriched in a variety of gene ontology terms and pathways. This finding suggests the epigenetic factor, microRNA, is highly likely involved in modulating vaccine protective efficacy in chicken.

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Section: Differentially Expressed Micrornas In Response To Hvt or CVImentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Marek’s disease vaccines-induced differential expression of known and novel microRNAs in primary lymphoid organ bursae of White Leghorn

Zhang

Zhu

Heidari

et al. 2020

Vet Res

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“…MD resistance in chickens is generally evaluated with MD incidence (induced gross tumors by MDV) and survival days post MDV challenge. The calculation was done as the ratio of number of birds with tumors/total number of birds challenged within each of the lines multiplied by 100 (cited from Xie et al [27]) (THRSP and β-actin). We found that all of the 10 CNVRs were confirmed in agreement with the CNVnator results ( Fig.…”

Section: Cnvrs Validationmentioning

confidence: 99%

Genome-wide characterization of copy number variations in the host genome in genetic resistance to Marek’s disease using next generation sequencing

Bai

Ding

et al. 2020

BMC Genet

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Background: Marek's disease (MD) is a highly neoplastic disease primarily affecting chickens, and remains as a chronic infectious disease that threatens the poultry industry. Copy number variation (CNV) has been examined in many species and is recognized as a major source of genetic variation that directly contributes to phenotypic variation such as resistance to infectious diseases. Two highly inbred chicken lines, 6 3 (MD-resistant) and 7 2 (MD-susceptible), as well as their F 1 generation and six recombinant congenic strains (RCSs) with varied susceptibility to MD, are considered as ideal models to identify the complex mechanisms of genetic and molecular resistance to MD. Results: In the present study, to unravel the potential genetic mechanisms underlying resistance to MD, we performed a genome-wide CNV detection using next generation sequencing on the inbred chicken lines with the assistance of CNVnator. As a result, a total of 1649 CNV regions (CNVRs) were successfully identified after merging all the nine datasets, of which 90 CNVRs were overlapped across all the chicken lines. Within these shared regions, 1360 harbored genes were identified. In addition, 55 and 44 CNVRs with 62 and 57 harbored genes were specifically identified in line 6 3 and 7 2 , respectively. Bioinformatics analysis showed that the nearby genes were significantly enriched in 36 GO terms and 6 KEGG pathways including JAK/STAT signaling pathway. Ten CNVRs (nine deletions and one duplication) involved in 10 disease-related genes were selected for validation by using quantitative real-time PCR (qPCR), all of which were successfully confirmed. Finally, qPCR was also used to validate two deletion events in line 7 2 that were definitely normal in line 6 3. One high-confidence gene, IRF2 was identified as the most promising candidate gene underlying resistance and susceptibility to MD in view of its function and overlaps with data from previous study. Conclusions: Our findings provide valuable insights for understanding the genetic mechanism of resistance to MD and the identified gene and pathway could be considered as the subject of further functional characterization.

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Immunogenetics and the mapping of immunological functions

et al. 2022

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Genomic Variation between Genetic Lines of White Leghorns Differed in Resistance to Marek’s Disease

Cited by 7 publications

References 48 publications

Marek’s disease vaccines-induced differential expression of known and novel microRNAs in primary lymphoid organ bursae of White Leghorn

Marek’s disease vaccines-induced differential expression of known and novel microRNAs in primary lymphoid organ bursae of White Leghorn

Genome-wide characterization of copy number variations in the host genome in genetic resistance to Marek’s disease using next generation sequencing

Immunogenetics and the mapping of immunological functions

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