Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers

Miller, A M; Figueiredo, J. E. F.; Chaves, Carolina de Andrade Lima; Ruas, Eduardo Augusto; Balbi-Peña, María Isabel; Colauto, Nelson Barros; Paccola-Meirelles, Luzia Doretto

doi:10.4238/gmr15049452

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…The genetic diversity of 11 cultivars of soybean from 18 AFLP primer combinations has been reported with 43% polymorphism [40]. AFLP markers have been used to determine the genetic diversity for the conservation of endangered species, such as Australian hidden beard heath (Leucopogon obtectus), spring pasqueflower (Pulsatilla vernalis) in Europe, and Chascolytrum bulbosum, a grass species that is native to Latin America [41][42][43][44][45]; germplasm collection [46]; and the diversity of plant-related pathogens [47].…”

Section: Amplified Fragment Length Polymorphismmentioning

confidence: 99%

Assessment of plant genetic variations using molecular markers: A review

Hussain¹,

Nisar²

2020

J Appl Biol Biotech

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Climatic changes affect various organisms, including plant species, becoming unfavorable for the environment and socioeconomic value, prompting an increase in activities related to plant resources conservation. The successful conservation of plant genetic resources depends on the proper identification and characterization of plant material. One of the notable developments in genetic conservation is the use of molecular markers for assessing the conservation and use of plant genetic resources. This review is devoted to the use of molecular marker techniques for genetic assessment of plant genetic variations. Development in these techniques provides smooth, reliable, and effortless ways for assessing known and unknown taxa, between and within species. These techniques provide a revelation to researchers on taxonomical and evolutionary questions which were not possible earlier. The polymerase chain reaction-based molecular markers give rise to various novel techniques due to the simplicity and high reproducibility of the methods. Examples including a combination of earlier techniques, such as restriction fragment length polymorphism, random amplification of polymorphic DNA, simple sequence repeat, intersimple sequence repeats, single nucleotide polymorphism, and amplified fragment length polymorphism, have been used for plant genetic variations and polymorphism studies. Progress in the advanced high-throughput sequencing techniques or next-generation sequencing technologies has been rapidly utilized to study genetic diversity broadly and to identify suitable genes and alleles rapidly. These techniques offer a practical resolution to the challenges in crop genomics. This review explains the recent advances in the molecular marker techniques, along with the advantages, uses, and limitations. Each technique differs in resolving the genetic variations and polymorphism in plant species.

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Section: Amplified Fragment Length Polymorphismmentioning

confidence: 99%

Assessment of plant genetic variations using molecular markers: A review

Hussain¹,

Nisar²

2020

J Appl Biol Biotech

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“…P. ananatis is reported to cause center rot disease of onion, and accounted for 100% loss in some fields (Gitaitis & Gay, 1997). While maize white spot (MWS) caused by this pathogen could cause up to 60% yield loss (Miller et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Pantoea ananatis Isolated from Garlic and Shallot

Nurjanah¹,

Joko

Subandiyah

2018

JPTI

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The new disease on garlic (Allium sativum) and shallot (A. cepa L. aggregatum group) have been found in several production centers of garlic and shallot in Tawangmangu and Temanggung, Central Java. The infected plants showed symptoms of leaf blight accompanied by chlorosis. The objective of this study was to determine the pathogen that causes leaf blight and chlorosis based on the phenotypic characterization and gyrB gene sequences analysis. The research started from the isolation of pathogen, physiological and biochemical test, DNA extraction, and sequence analysis of gyrB using gyrB 01-F and gyrB 02-R primer. The results showed that the isolated bacterial pathogen have a yellow pigment, slimy colonies with regular borders, convex, gram-negative, non-spore, facultative anaerobic, motile, catalase production, indole production, and acid production from D-glucose, D-mannitol, sucrose, and lactose. From the pathogenicity test, it was found that the bacteria produced the typical symptom of leaf blight. Characterization of pathogens based on gyrB gene sequence revealed that the pathogen was placed in the group of Pantoea ananatis. Keywords: Alium cepa, Alium sativum, gyrB, leaf blight, Pantoea ananatis INTISARIPenyakit baru pada bawang putih (Allium sativum) dan bawang merah (A. cepa L. aggregatum group) telah ditemukan di beberapa sentra produksi bawang putih dan bawang merah di Tawangmangu dan Temanggung, Jawa Tengah. Tanaman yang terinfeksi menunjukkan gejala hawar daun disertai klorosis. Tujuan penelitian untuk mengetahui karakter patogen berdasarkan fenotipik dan sekuen gen gyrB. Penelitian dimulai dengan isolasi bagian tanaman yang sakit, uji fisiologi dan biokimia, ekstraksi DNA dengan metode CTAB/NaCl dan amplifikasi gen gyrB menggunakan primer gyrB cembung, pigmen berwarna kuning, gram negative, tidak berspora, aerob fakultatif, motil, produksi katalase, indol, sukrosa

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“…Esta variação genética expressada geralmente pode ser reconhecida por diferenças em moléculas de proteínas, diversas características fisiológicas, bioquímicas e também por variações comportamentais e morfológicas (SANTOS et al, 2009). A falta de conhecimento da diversidade genética de P. ananatis têm sido um desafio para o desenvolvimento de genótipos resistentes à doença, resultando assim em cultivares com resistência de curto prazo e ineficientes (MILLER et al, 2016). Segundo Souza e Duarte (2002) a resistência genética de P. ananatis é instável, pois os níveis de resistência de um híbrido de milho podem variar em diferentes ambientes.…”

Section: Diversidade Genética De Pantoea Ananatisunclassified

Agressividade, caracterização molecular de isolados de Pantoea ananatis e nanoparticulas no controle da bactéria na cultura do milho

Mota¹

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The maize white spot, caused by Pantoea ananatis stands out as one of the main foliar diseases of culture. For the management of this disease it is recommended the use of resistant genotypes and chemicals that there has been presenting efficiency, therefore a technological innovation in chemical control of phytopathogens should be evaluated in the control of the disease.Thus, the present work had as objectives: to assess the aggressiveness of isolates of P. ananatis; molecularly characterize isolates of P. ananatis and to evaluate the effect of nanoparticles in control of bacteria in corn plants For the evaluation of aggressiveness were used nine isolates of P. ananatis inoculated in three maize genotypes (DKB230, AG9025, 2B810PW), with four replications. The severity of white spot of leaves was evaluated at 2, 4, 6 and 8 days after inoculation, using a scale of note; for molecular characterization werw studied 23 isolates of P. ananatis using the specific primers ANAF/ANAR and universal BOX. To control with nanoparticles were used nanocrystals of (ZnO) doped with four elements-copper (Cu), iron (Fe), manganese (Mn), and nickel (Ni)-in different concentrations and in two distinct forms of control. The bacterial isolates UFU18 and UFU B13 were more aggressive in maize genotypes susceptible (DKB230, AGV9025), and in the resistant genotype (2B810PW) there was no disease development of the disease. The isolates were identified as P. ananatis using specific primer and allowed the study of genetic diversity of the isolates with primer BOX. The use of nanocrystals of zinc oxide (ZnO) doped with copper (Cu) and manganese (Mn) reduced disease severity in maze plants in greenhouse.

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Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers

Cited by 5 publications

References 24 publications

Assessment of plant genetic variations using molecular markers: A review

Assessment of plant genetic variations using molecular markers: A review

Characterization of Pantoea ananatis Isolated from Garlic and Shallot

Agressividade, caracterização molecular de isolados de Pantoea ananatis e nanoparticulas no controle da bactéria na cultura do milho

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