Genomic typing of Streptococcus uberis isolates from cases of mastitis, in New Zealand dairy cows, using pulsed-field gel electrophoresis

Douglas, Vicki L; Fenwick, Stan; Pfeiffer, Dirk U.; Williamson, N. B.; Holmes, C. W.

doi:10.1016/s0378-1135(00)00184-x

Cited by 52 publications

(63 citation statements)

References 33 publications

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“…PFGE demonstrated a high discriminatory index (0.9889) in accordance with previous studies (DOUGLAS et al, 2000;LASAGNO et al, 2011;PHUEKTES et al, 2001;RATO et al, 2008RATO et al, , 2013. Four pulse-types found by PFGE (VI, VII-a, VIII, and IX) showed two strains with similar patterns that were classified as having identical restriction patterns (Figure 1).…”

Section: Resultssupporting

confidence: 89%

“…Surveys of dairy cattle worldwide showed an increase in mastitis caused by S. uberis, especially in intensively managed herds (ALMEIDA et al, 2006;COSTA unpublished data;DOUGLAS et al, 2000;PHUEKTES et al, 2001). Despite the economic impact caused by this bacterium in well-managed dairy herds, the pathogenesis of the disease and the relationship between genetic diversity and virulence genes is poorly studied.…”

Section: Introductionmentioning

confidence: 98%

“…These approaches may permit the development of more effective control measures for this microorganism. Among the different molecular typing methods, the Pulsed Field Gel Electrophoresis (PFGE) technique has been used to study pathogens involved in the etiology of bovine mastitis because it is highly discriminatory and highly reproducible (BASEGGIO et al, 1997;DOUGLAS et al, 2000;LASAGNO et al, 2011;PHUEKTES et al, 2001;RATO et al, 2008RATO et al, , 2013. According to Shome et al (2012), PFGE is the technique of choice for short-term or local epidemiological analyses.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Loures¹,

Pereira²,

Carvalho-Castro³

et al. 2017

SCA

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ResumoA mastite é uma das doenças infecciosas mais onerosas em bovinos leiteiros em todos os continentes. Trata-se de uma doença de cunho multifatoral causada por diferentes microrganismos, incluindo vírus, leveduras, algas, parasitas e várias espécies de bactérias. Entre as bactérias, Streptococcus uberis destacase como um importante agente ambiental, responsável por uma grande variedade de infecções clínicas e subclínicas da glândula mamária, especialmente em sistemas de produção intensivos. Apesar da crescente importância de S. uberis na etiologia da mastite bovina, existem poucos estudos sobre a diversidade populacional e fatores de virulência deste patógeno em rebanhos leiteiros brasileiros.Os objetivos do presente estudo foram investigar as características de virulência e a epidemiologia molecular de S. uberis isolados de mastite bovina em rebanhos brasileiros, utilizando-se a PCR e a eletroforese em gel de campo pulsado (PFGE). Para tal, 46 amostras de S. uberis isoladas de mastite bovina em 26 rebanhos leiteiros de uma mesma mesorregião brasileira foram avaliadas quanto à diversidade populacional por eletroforese em gel de campo pulsado (PFGE), utilizando-se a enzima Smal. Além disso, foi realizada PCR para detectar os genes de virulência pauA e skc, que codificam ativadores de plasminogênio, e do gene sua, que codifica uma proteína de adesão às celulas epiteliais da glândula mamária. Nossos resultados mostraram uma alta diversidade genética na população estudada, com vários padrões de PFGE, e uma elevada frequência dos genes de virulência pesquisados: sua (100%), pauA (91%) e skc (91%). A alta frequência dos genes skc, pauA e sua sugere a importância destes fatores de virulência para S. uberis na colonização da glândula mamária bovina. Estudos moleculares e genéticos sobre este agente podem contribuir muito para melhorar o conhecimento sobre a patogenia, permitindo identificar moléculas que tenham papel relevante no estabelecimento da infecção, fornecendo informações importantes para a adoção de medidas mais eficazes para a prevenção e o controle da mastite bovina. AbstractMastitis is one of the most common and costly infectious diseases in dairy cattle worldwide. This is a multifactorial illness caused by different microorganisms, including virus, yeasts, algae, parasites, and several species of bacteria. Among these bacteria, Streptococcus uberis is an important environmental pathogen that is responsible for a large range of clinical and subclinical mammary infections, especially in intensively managed herds. Despite the increasing importance of this pathogen in the etiology of bovine mastitis, data on its virulence and diversity in Brazilian dairy herds are scarce. The aims of the present study were to investigate the virulence characteristics of S. uberis isolated from bovine mastitis and to assess the molecular epidemiology of the Brazilian isolates using pulsed-field gel electrophoresis (PFGE). In this work, 46 strains of S. uberis isolated from bovine mastitis from 26 Brazilian dairy herds were ...

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Loures¹,

Pereira²,

Carvalho-Castro³

et al. 2017

SCA

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“…The strain collection contained 57 different sequence types (STs), showing that the population was heterogeneous. This is in agreement with a number of other studies using different typing methods to differentiate sets of S. uberis isolates (5,10,15,17). However, the MLST analysis, unlike most previous typing methods, provided useful information about the genetic relationships between the strains.…”

supporting

confidence: 90%

“…S. uberis is also highly prevalent in Australia and New Zealand, where the dairy industry is mainly pasture based (13,14). A recent report from New Zealand indicated that over 30% of all clinical mastitis was due to this organism (5).…”

mentioning

confidence: 99%

Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom

Pullinger

Lopez-Benavides²,

Coffey

et al. 2006

Appl Environ Microbiol

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We recently developed a multilocus sequence typing (MLST) scheme to differentiate S. uberis isolates and facilitate an understanding of the population biology of this pathogen. The scheme was initially used to study a collection of 160 bovine milk isolates from the United Kingdom and showed that the majority of isolates were from one clonal complex (designated the ST-5 complex). Here we describe the MLST analysis of a collection of New Zealand isolates. These were obtained from diverse sources, including bovine milk, other bovine anatomical sites, and environmental sources. The complete allelic profiles of 253 isolates were determined. The collection was highly diverse and included 131 different sequence types (STs). The New Zealand and United Kingdom populations were distinct, since none of the 131 STs were represented within the previously studied collection of 160 United Kingdom S. uberis isolates. However, seven of the STs were members of the ST-5 clonal complex, the major complex within the United Kingdom collection. Two new clonal complexes were identified: ST-143 and ST-86. All three major complexes were isolated from milk, other bovine sites, and the environment. Carriage of the hasA gene, which is necessary for capsule formation, correlated with clonal complex and isolation from clinical cases of mastitis.

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Bovine Mastitis and Biofilms

Melchior¹

2011

Springer Series on Biofilms

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Biofilm formation in bovine mastitis Staphylococcus aureus isolates was studied since the beginnings of biofilm research, even before the name "biofilm" was actually invented. Compared to other major bovine mastitis pathogens, such as E. coli and Streptococcus uberis relatively much research information is available on S. aureus biofilm formation, biofilm antimicrobial susceptibility and the role of several biofilm related genes.Recent research on biofilm formation from E. coli and Str. uberis shed an interesting light on the whole dynamic process of bacterial invasion, adherence, persistence and evasive strategies of these bacteria, and reveal parallels and differences between these bacteria and S. aureus. In this chapter we present the current knowledge on biofilm formation in the bovine udder as an holistic and dynamic process, with more or less strain specific adaptive strategies and mechanisms which all support perseverance and survival of these bacterial species under stressful circumstances.

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Genomic typing of Streptococcus uberis isolates from cases of mastitis, in New Zealand dairy cows, using pulsed-field gel electrophoresis

Cited by 52 publications

References 33 publications

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom

Bovine Mastitis and Biofilms

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