Genomic transcriptional profiling identifies a candidate blood biomarker signature for the diagnosis of septicemic melioidosis

Pankla, Rungnapa; Buddhisa, Surachat; Berry, Matthew; Blankenship, Derek; Bancroft, Gregory J.; Banchereau, Jacques; Lertmemongkolchai, Ganjana; Chaussabel, Damien

doi:10.1186/gb-2009-10-11-r127

Cited by 171 publications

(184 citation statements)

References 51 publications

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“…The greater distance of influenza virus samples from mock samples in the condition tree compared to RSV samples suggested that the transcriptional changes on hAECs were more prominent during influenza virus infection than during RSV infection. To measure the magnitude of the gene expression changes induced by each viral infection, we converted transcript abundance into MDTH, a representative score indicating the degree of transcriptional perturbation for a given sample compared to its control (35). MDTH values were higher in influenza virus-infected hAECs than in those infected with RSV (Fig.…”

Section: Resultsmentioning

confidence: 99%

Plasticity and Virus Specificity of the Airway Epithelial Cell Immune Response during Respiratory Virus Infection

Ioannidis

McNally

Willette

et al. 2012

J Virol

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181

174

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Section: Resultsmentioning

confidence: 99%

Plasticity and Virus Specificity of the Airway Epithelial Cell Immune Response during Respiratory Virus Infection

Ioannidis

McNally

Willette

et al. 2012

J Virol

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181

174

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“…False-negative molecular detection of B. pseudomallei has been attributed to lower concentrations of the organism in blood (Supaprom et al, 2007). Whilst more complex methods, such as genomic transcription profiling using microarray technology have shown greater promise, this technology is generally neither available nor feasible in regions where this disease is endemic (Pankla et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay

Robertson

Sorenson

Govan

et al. 2015

Journal of Medical Microbiology

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The rapid diagnosis of septicaemic melioidosis will have an impact on reduction of mortality. Currently, this relies almost exclusively upon culture of the causative agent Burkholderia pseudomallei from clinical samples. In acute sepsis, blood is the preferred specimen for culture and therefore should be the target for a rapid diagnostic tool. A lateral flow immunoassay (LFI) for the detection of B. pseudomallei antigen has been developed. This was compared with molecular detection using the targets T3SS1 and IpxO. Forty-five clinical samples of EDTA blood, which were culture-positive, were tested using both modalities. The LFI had a sensitivity of 40 %, whilst molecular detection had a sensitivity of 20 %. The poor performance of molecular detection has been described previously and is largely related to the use of wholeblood specimens collected into blood tubes containing EDTA. Whilst suboptimal, the LFI would be an adjunct in the rapid diagnosis of melioidosis.

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“…However, genome array studies in the peripheral blood of patients with melioidosis suggest that T cell functions may be impaired, with underexpression of transcripts related to T cells and cytotoxic cells. In contrast, overexpression of transcripts corresponding to PMN functions including the abundance of immature neutrophils (e.g., DEFA1, DEFA3, FALL-39) and genes encoding neutrophil cell-surface markers (such as ITGAM [CD11b], FCGR1 [CD64], CD62L, and CSF3R) was observed in blood of patients with sepsis compared with uninfected control subjects (17). We believe that PMNs may have multiple cytokine or cell contact-mediated immune-regulatory interactions with other cells of the immune response during B. pseudomallei infection, but to date this has not been investigated.…”

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confidence: 99%

Programmed Death Ligand 1 on Burkholderia pseudomallei–Infected Human Polymorphonuclear Neutrophils Impairs T Cell Functions

Buddhisa

Rinchai

Ato

et al. 2015

The Journal of Immunology

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Polymorphonuclear neutrophils (PMNs) are terminally differentiated cells that are involved in innate immune responses and form an early line of defense against pathogens. More recently, it has been shown that PMNs have immunosuppressive abilities on other immune cells. However, the effect of PMNs on T cell responses during bacterial infection remains to be determined. In this report, we examined the interaction of PMNs and T cells in response to infection with Burkholderia pseudomallei, the causative agent of human melioidosis. We observed that CD4+ T cell proliferation and IFN-γ production in response to polyclonal activators is significantly inhibited by uninfected PMNs, and to a greater extent B. pseudomallei–infected PMNs. Programmed death ligand 1 (PD-L1), a known regulator of T cell activation, is increased in mRNA expression in the blood of patients and upon infection of PMNs in vitro. The increased expression of PD-L1 was correlated with the degree of T cell inhibition in individuals with type 2 diabetes, a major risk factor of melioidosis. In vitro, addition of anti–PD-L1 Abs blocked this inhibitory activity and restored proliferation of CD4+ T cells and IFN-γ production, suggesting that PD-L1 on B. pseudomallei–infected PMNs is a regulatory molecule for the functions of T cells and may be involved in pathogenesis versus control of melioidosis.

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Genomic transcriptional profiling identifies a candidate blood biomarker signature for the diagnosis of septicemic melioidosis

Abstract: A diagnostic signature for sepsis caused by Burkholderia pseudomallei infection was identified from transcriptional profiling of the blood of septicemia patients.

Cited by 171 publications

References 51 publications

Plasticity and Virus Specificity of the Airway Epithelial Cell Immune Response during Respiratory Virus Infection

Plasticity and Virus Specificity of the Airway Epithelial Cell Immune Response during Respiratory Virus Infection

Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay

Programmed Death Ligand 1 on Burkholderia pseudomallei–Infected Human Polymorphonuclear Neutrophils Impairs T Cell Functions

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