Genomic tests: unreliable for cancer? A focus on circulating DNA and lung cancer

Teare, M. Dawn; Woll, Penella J.

doi:10.1586/14737159.7.6.699

Cited by 9 publications

(2 citation statements)

References 13 publications

(22 reference statements)

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“…Recent interest in the study of circulating cell free DNA has led to interest in it as a screening tool for lung cancer [7,8]. Circulating cell free DNA is extracellular DNA found in cell free plasma/serum of humans, plants and in the animal kingdom [9,10].…”

Section: Introductionmentioning

confidence: 99%

Value of quantitative analysis of circulating cell free DNA as a screening tool for lung cancer: A meta-analysis

Zhang

Shao

et al. 2010

Lung Cancer

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Section: Introductionmentioning

confidence: 99%

Value of quantitative analysis of circulating cell free DNA as a screening tool for lung cancer: A meta-analysis

Zhang

Shao

et al. 2010

Lung Cancer

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“…Previous work has investigated plasma and serum levels of deoxyribonucleic acid (DNA), and ribonucleic acid (RNA) in attempts to detect the presence of cancer [ 2 - 5 ]. It has been reported that serum and plasma samples from cancer patients generally have higher concentrations of these nucleic acids compared with control samples.…”

Section: Introductionmentioning

confidence: 99%

Potential for Raman spectroscopy to provide cancer screening using a peripheral blood sample

et al. 2009

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Cancer poses a massive health burden with incidence rates expected to double globally over the next decade. In the United Kingdom screening programmes exists for cervical, breast, and colorectal cancer. The ability to screen individuals for solid malignant tumours using only a peripheral blood sample would revolutionise cancer services and permit early diagnosis and intervention. Raman spectroscopy interrogates native biochemistry through the interaction of light with matter, producing a high definition biochemical 'fingerprint' of the target material. This paper explores the possibility of using Raman spectroscopy to discriminate between cancer and noncancer patients through a peripheral blood sample. Forty blood samples were obtained from patients with Head and Neck cancer and patients with respiratory illnesses to act as a positive control. Raman spectroscopy was carried out on all samples with the resulting spectra being used to build a classifier in order to distinguish between the cancer and respiratory patients' spectra; firstly using principal component analysis (PCA)/linear discriminant analysis (LDA), and secondly with a genetic evolutionary algorithm. The PCA/LDA classifier gave a 65% sensitivity and specificity for discrimination between the cancer and respiratory groups. A sensitivity score of 75% with a specificity of 75% was achieved with a 'trained' evolutionary algorithm. In conclusion this preliminary study has demonstrated the feasibility of using Raman spectroscopy in cancer screening and diagnostics of solid tumours through a peripheral blood sample. Further work needs to be carried out for this technique to be implemented in the clinical setting.

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Circulating nucleic acids in cancer and pregnancy

Pinzani

Salvianti

Pazzagli

et al. 2010

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Genomic tests: unreliable for cancer? A focus on circulating DNA and lung cancer

Cited by 9 publications

References 13 publications

Value of quantitative analysis of circulating cell free DNA as a screening tool for lung cancer: A meta-analysis

Value of quantitative analysis of circulating cell free DNA as a screening tool for lung cancer: A meta-analysis

Potential for Raman spectroscopy to provide cancer screening using a peripheral blood sample

Circulating nucleic acids in cancer and pregnancy

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