Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014

Rodrigues, Ayslany Melo; Souza, Rafael Ribeiro Mota; Fonseca, Larissa Moraes dos Santos; Rolo, Carolina de Araújo; Carvalho, Renata; Sardi, Sílvia Inês; Campos, Gúbio Soares

doi:10.1590/0037-8682-0583-2019

Cited by 7 publications

(3 citation statements)

References 14 publications

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“…A systematic review showed that the ECSA-diverged/ Indian Ocean Lineage (IOL) genotype seems to generate chronic cases more often (50%) [ 27 ]. In our study, as in other studies carried out in Brazil [ 33 , 44 – 47 ], the genotype was ECSA and, although the percentage of patients with pCHIKV-CIJD exceeded 45%, we did not find any unique mutations that could be related to prognosis. Similarly, we were not able to demonstrate correlation of early CHIKV viral load with chronic disease, despite previous studies which had shown it before [ 39 , 40 ].…”

Section: Discussionsupporting

confidence: 84%

Clinical markers of post-Chikungunya chronic inflammatory joint disease: A Brazilian cohort

et al. 2023

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Background Chikungunya-fever (CHIKF) remains a public health major issue. It is clinically divided into three phases: acute, post-acute and chronic. Chronic cases correspond to 25–40% individuals and, though most of them are characterized by long-lasting arthralgia alone, many of them exhibit persistent or recurrent inflammatory signs that define post-Chikungunya chronic inflammatory joint disease (pCHIKV-CIJD). We aimed to identify early clinical markers of evolution to pCHIKV-CIJD during acute and post-acute phases. Methodology/Principal findings We studied a prospective cohort of CHIKF-confirmed volunteers with longitudinal clinical data collection from symptoms onset up to 90 days, including a 21-day visit (D21). Of 169 patients with CHIKF, 86 (50.9%) completed the follow-up, from whom 39 met clinical criteria for pCHIKV-CIJD (45.3%). The relative risk of chronification was higher in women compared to men (RR = 1.52; 95% CI = 1.15–1.99; FDR = 0.03). None of the symptoms or signs presented at D0 behaved as an early predictor of pCHIKV-CIJD, while being symptomatic at D21 was a risk factor for chronification (RR = 1.31; 95% CI = 1.09–1.55; FDR = 0.03). Significance was also observed for joint pain (RR = 1.35; 95% CI = 1.12–1.61; FDR = 0.02), reported edema (RR = 3.61; 95% CI = 1.44–9.06; FDR = 0.03), reported hand and/or feet small joints edema (RR = 4.22; 95% CI = 1.51–11.78; FDR = 0.02), and peri-articular edema observed during physical examination (RR = 2.89; 95% CI = 1.58–5.28; FDR = 0.002). Furthermore, patients with no findings in physical examination at D21 were at lower risk of chronic evolution (RR = 0.41, 95% CI = 0.24–0.70, FDR = 0.01). Twenty-nine pCHIKV-CIJD patients had abnormal articular ultrasonography (90.6% of the examined). The most common indings were synovitis (65.5%) and joint effusion (58.6%). Conclusion This cohort has provided important insights into the prognostic evaluation of CHIKF. Symptomatic sub-acute disease is a relevant predictor of evolution to chronic arthritis with synovitis, drawing attention to joint pain, edema, multiple articular involvement including small hand and feet joints as risk factors for chronification beyond three months, especially in women. Future studies are needed to accomplish the identification of accurate and early biomarkers of poor clinical prognosis, which would allow better understanding of the disease’s evolution and improve patients’ management, modifying CHIKF burden on global public health.

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Section: Discussionsupporting

confidence: 84%

Clinical markers of post-Chikungunya chronic inflammatory joint disease: A Brazilian cohort

et al. 2023

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“…The Asian/Caribbean lineage (CHIKV-Asian), first identified in September 2014 in the Oiapoque municipality, state of Amapá, followed by the East-Central-South African (CHIKV-ECSA) lineage, which was introduced in Brazil in the city of Feira-de-Santana, state of Bahia [ 14 , 15 ]. Since then, several autochthonous cases have been described [ 5 , 16 ]. Likewise, several imported cases were reported [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Chikungunya Virus Asian Lineage Infection in the Amazon Region Is Maintained by Asiatic and Caribbean-Introduced Variants

Ribeiro

Gill

Ramos

et al. 2022

Viruses

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The simultaneous transmission of two lineages of the chikungunya virus (CHIKV) was discovered after the pathogen’s initial arrival in Brazil. In Oiapoque (Amapá state, north Brazil), the Asian lineage (CHIKV-Asian) was discovered, while in Bahia state, the East-Central-South-African lineage (CHIKV-ECSA) was discovered (northeast Brazil). Since then, the CHIKV-Asian lineage has been restricted to the Amazon region (mostly in the state of Amapá), whereas the ECSA lineage has expanded across the country. Despite the fact that the Asian lineage was already present in the Amazon region, the ECSA lineage brought from the northeast caused a large outbreak in the Amazonian state of Roraima (north Brazil) in 2017. Here, CHIKV spread in the Amazon region was studied by a Zika–Dengue–Chikungunya PCR assay in 824 serum samples collected between 2013 and 2016 from individuals with symptoms of viral infection in the Amapá state. We found 11 samples positive for CHIKV-Asian, and, from these samples, we were able to retrieve 10 full-length viral genomes. A comprehensive phylogenetic study revealed that nine CHIKV sequences came from a local transmission cluster related to Caribbean strains, whereas one sequence was related to sequences from the Philippines. These findings imply that CHIKV spread in different ways in Roraima and Amapá, despite the fact that both states had similar climatic circumstances and mosquito vector frequencies.

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“…A rapid onset of disease with no pro-dermal phase, following an incubation period of 2-4 days. [1][2][3] CHIKV enters into the target cells via endocytosis. Upon endocytosis, endosomes undergo conformational change in acidic environments in viral envelope and exposes E1 peptide, mediating virus-host cell membrane fusion.…”

Section: Introductionmentioning

confidence: 99%

Impact of Temperature on Structural Changes in nsp2 and nsp3 of CHIKV: Molecular Dynamics Simulations

Kumar

Meena

Kumar

et al. 2022

Preprint

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Authors have considered non-structural protease-2 (nsp2) and non-structural protease-3 (nsp3) of CHIKV for the investigation as they are involved in the replication of the virus and increases the infections in the human. CHIKV protease (nsp2), has a catalytic dyad (Cys1013/His1083) that processes viral polyproteins. Further, it has also been suggested that the catalytic dyad is interchangeable to a serine (proximal) and hence making called non-papain-like cysteine protease. Similarly, nsp3 plays important role during replication of CHIKV genome. In this work, we have investigated the effect of temperature on the structural stability of nsp2 and nsp3 of CHIKV. Although, we did not get any regular patter for change in the RMSD and RMSF for the nsp3 of CHIKV and observed that RMSD for nsp3 of CHIKV is acceptable at all the temperature (300, 325 and 350 K) but the minimum RMSD values are obtained at 325 K. Similarly, no regular pattern is observed for RMSF trajectories of nsp3 of CHIKV at different temperature and minimum fluctuations in the position of amino-acids at 325 K. Further, the RMSD and RMSF trajectories for nsp2 of CHIKV are investigated and observed a regular pattern in the RMSD and RMSF trajectories generated from MD simulations on increasing the temperature. In this, RMSD values are minimum at 300 K while the fluctuation in the position of amino-acids are maximum.

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Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014

Cited by 7 publications

References 14 publications

Clinical markers of post-Chikungunya chronic inflammatory joint disease: A Brazilian cohort

Clinical markers of post-Chikungunya chronic inflammatory joint disease: A Brazilian cohort

Chikungunya Virus Asian Lineage Infection in the Amazon Region Is Maintained by Asiatic and Caribbean-Introduced Variants

Impact of Temperature on Structural Changes in nsp2 and nsp3 of CHIKV: Molecular Dynamics Simulations

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