Genomic surveillance links livestock production with the emergence and spread of multi-drug resistant non-typhoidal Salmonella in Mexico

Delgado-Suárez, Enrique Jesús; Ortı́z-López, Rocio; Gebreyes, Wondwossen A.; Allard, Marc W.; Barona‐Gómez, Francisco; Lozano, María Salud Rubio

doi:10.1007/s12275-019-8421-3

Cited by 10 publications

(20 citation statements)

References 42 publications

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“…The rate of resistance to penicillins observed here was not as high as that reported recently in NTS isolated from beef in Mexico [14,15,17,56]. However, this variation is likely associated with the region where isolates originated.…”

Section: Discussioncontrasting

confidence: 66%

“…Recent studies from Mexico have also documented high rates (36-44%) of decreased susceptibility to ciprofloxacin in beef NTS isolates [12], although these authors did not determine AMR genotypes. Moreover, a previous study by our research group documented qnr and oqx alleles were widely distributed among NTS isolated from cattle feces, carcasses and ground beef [17]. Furthermore, genomic AMR profiling of public NTS isolates showed qnr alleles are widely disseminated among bovine and avian isolates.…”

Section: Plos Onementioning

confidence: 75%

“…Our results confirmed previous observations of widespread resistance to older antibiotics (i. e. tetracyclines, penicillins) among NTS from different sources. For instance, in Mexico, previous studies consistently report these phenotypes at high frequencies (up to 90% or higher) in beef isolates [12,15,17,[49][50][51]. Likewise, these AMR phenotypes are also common in NTS isolated in developed countries, a phenomenon that is considered driven by the use of these antimicrobials in food-producing animals [27].…”

Section: Discussionmentioning

confidence: 92%

“…Regarding aminoglycoside resistance, although the number of non-susceptible isolates in the experiment was low, the CLSI guidelines emphasize these antimicrobials may appear active in vitro but are not effective clinically against Salmonella and, thus, susceptible isolates should not be reported as such [30]. This situation may explain why isolates carrying aadA and aph alleles exhibited susceptible phenotypes, a phenomenon observed in previous experiments [17]. However, comparative genomics of public NTS isolated in Mexico showed poultry and cattle are as important as humans as a source of aminoglycoside resistant genes.…”

Section: Plos Onementioning

confidence: 99%

“…Such information is crucial to devise new strategies to contain the dissemination of AMR pathogens. However, it can best be obtained by addressing phenotypic and genomic profiling of AMR simultaneously, an area with a limited number of studies in Mexico and other countries [17].…”

Section: Introductionmentioning

confidence: 99%

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Genomic surveillance of antimicrobial resistance shows cattle and poultry are a moderate source of multi-drug resistant non-typhoidal Salmonella in Mexico

et al. 2021

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Multi-drug resistant (MDR) non-typhoidal Salmonella (NTS) is a public health concern globally. This study reports the phenotypic and genotypic antimicrobial resistance (AMR) profiles of NTS isolates from bovine lymph nodes (n = 48) and ground beef (n = 29). Furthermore, we compared genotypic AMR data of our isolates with those of publicly available NTS genomes from Mexico (n = 2400). The probability of finding MDR isolates was higher in ground beef than in lymph nodes:χ2 = 12.0, P = 0.0005. The most common resistant phenotypes involved tetracycline (40.3%), carbenicillin (26.0%), amoxicillin-clavulanic acid (20.8%), chloramphenicol (19.5%) and trimethoprim-sulfamethoxazole (16.9%), while more than 55% of the isolates showed decreased susceptibility to ciprofloxacin and 26% were MDR. Conversely, resistance to cephalosporins and carbapenems was infrequent (0–9%). MDR phenotypes were strongly associated with NTS serovar (χ2 = 24.5, P<0.0001), with Typhimurium accounting for 40% of MDR strains. Most of these (9/10), carried Salmonella genomic island 1, which harbors a class-1 integron with multiple AMR genes (aadA2, blaCARB-2, floR, sul1, tetG) that confer a penta-resistant phenotype. MDR phenotypes were also associated with mutations in the ramR gene (χ2 = 17.7, P<0.0001). Among public NTS isolates from Mexico, those from cattle and poultry had the highest proportion of MDR genotypes. Our results suggest that attaining significant improvements in AMR meat safety requires the identification and removal (or treatment) of product harboring MDR NTS, instead of screening for Salmonella spp. or for isolates showing resistance to individual antibiotics. In that sense, massive integration of whole genome sequencing (WGS) technologies in AMR surveillance provides the shortest path to accomplish these goals.

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Section: Discussioncontrasting

confidence: 66%

Section: Plos Onementioning

confidence: 75%

Section: Discussionmentioning

confidence: 92%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genomic surveillance of antimicrobial resistance shows cattle and poultry are a moderate source of multi-drug resistant non-typhoidal Salmonella in Mexico

et al. 2021

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Withdrawn: Investigation on Salmonella enterica, Escherichia coli, and coliforms in beef from Ethiopian abattoirs: A potential risk of meat safety

Zelalem

Abegaz

Kebede

et al. 2022

Food Science & Nutrition

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The aims of this study were to determine the prevalence of Salmonella enterica (S. enterica) antimicrobial resistance based upon phenotypic assessment, and level of sanitation indicator organisms from 150 beef carcasses collected from three representative abattoirs in eastern, central, and southern Ethiopia. Samples were screened for S. enterica prevalence following the U.S. Department of Agriculture Microbiology Laboratory Guidebook (MLG) and confirmed by real‐time PCR. The S. enterica isolates were phenotypically evaluated for susceptibility to a panel of 13 antimicrobials using disk diffusion method as described in Clinical and Laboratory Standard Institutes guidelines. In addition, the coliform, total coliform, and generic E. coli populations were quantified by plating onto E. coli/coliform petrifilms. A total of 45 isolates of S. enterica were confirmed using real‐time PCR. The overall prevalence of S. enterica at the carcass level was 22.7% (95% CI, 16.0–30.0). However, based on the sampling points, S. enterica prevalence detected on hides was 13.3% (95% CI, 8.0–18.7), pre‐evisceration was 12.0% (95% CI, 7.3–17.3), and post‐evisceration was 4.7% (95% CI, 1.3–8.0). The prevalence of S. enterica was significantly lower on post‐evisceration as compared to hides (p = .009) and pre‐evisceration (p = .022). No differences were detected for the prevalence of S. enterica between abattoirs (p = .346). Finally, the prevalence of S. enterica was highest during the wet season (p = .011). The overall mean log CFU/cm2 ± SD of generic E. coli, coliform, and total coliform counts were 4.55 ± 0.99, 4.91 ± 1.13, and 4.98 ± 1.09, respectively. About 20% of S. enterica exhibited phenotypic multidrug resistant. The most frequently detected resistance was to Tetracycline (28.9%), followed by Streptomycin (22.2%) and Sulfisoxazole (20.0%). The data from this study highlight the need to implement interventions on improvement of sanitary practices in abattoirs.

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Prevalence and antimicrobial resistance of Listeria monocytogenes, Salmonella enterica and Escherichia coli O157:H7 in imported beef cattle in Jordan

Obaidat

2020

Comparative Immunology, Microbiology and Infectious Diseases

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Genomic surveillance links livestock production with the emergence and spread of multi-drug resistant non-typhoidal Salmonella in Mexico

Cited by 10 publications

References 42 publications

Genomic surveillance of antimicrobial resistance shows cattle and poultry are a moderate source of multi-drug resistant non-typhoidal Salmonella in Mexico

Genomic surveillance of antimicrobial resistance shows cattle and poultry are a moderate source of multi-drug resistant non-typhoidal Salmonella in Mexico

Withdrawn: Investigation on Salmonella enterica, Escherichia coli, and coliforms in beef from Ethiopian abattoirs: A potential risk of meat safety

Prevalence and antimicrobial resistance of Listeria monocytogenes, Salmonella enterica and Escherichia coli O157:H7 in imported beef cattle in Jordan

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