Genomic structures and characterization of the 5′-flanking regions of acyl carrier protein and Δ4-palmitoyl-ACP desaturase genes from Coriandrum sativum

Kim, Mi Jung; Shin, Jeong Sheop; Kim, Jeong-Kook; Suh, Mi Chung

doi:10.1016/j.bbaexp.2005.06.011

Cited by 10 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The CCAAT box, the E-box, and the RY elements in the ¡ 295phas promoter were also determined to be positively involved in the high expression levels observed in embryos (Chandrasekharan et al 2003). The similarity of cis-regulatory elements such as the GCN4-like motif (GTCA), G-box-like element (ACGT) or G-box (CACGCC), AACA motif, and Prolamin-box (AAAG) has also been reported to be located in the seed-speciWc promoters of acyl carrier proteins and 4 -palmitoyl-acyl carrier protein desaturase genes, which in turn are involved in the biosynthesis of petroselinic acid in Coriandrum sativum (Kim et al 2005). Therefore, future research should focus on deWning which cis-elements in a ¡ 179 bp section of the SeFAD2 promoter are associated with the quantitative regulation of SeFAD2 gene expression during seed development.…”

Section: Discussionmentioning

confidence: 97%

Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5′-UTR intron

et al. 2006

Self Cite

View full text Add to dashboard Cite

The regulation of genes involved in primary lipid metabolism in plants is much less well understood than that in many other pathways in plant biology. In the investigation reported here, we have characterized transcriptional regulatory mechanisms controlling seed-specific FAD2 expression in sesame (Sesamum indicum). FAD2 codes for extra-plastidial FAD2 desaturase, which catalyzes the conversion of oleic acid to linoleic acid. Promoter analysis of the sesame FAD2 gene (SeFAD2) using the beta-glucuronidase (GUS) reporter system demonstrated that the - 660 to - 180 promoter region functions as a negative cis-element in the seed-specific expression of the SeFAD2 gene. Sesame and Arabidopsis FAD2 genes harbor one large intron within their 5'-untranslated region. These introns conferred up to 100-fold enhancement of GUS expression in transgenic Arabidopsis tissues as compared with intron-less controls. Prerequisite cis-elements for the SeFAD2 intron-mediated enhancement of gene expression and the promoter-like activity of SeFAD2 intron were identified. SeFAD2 transcripts were induced by abscisic acid (ABA) in developing sesame seeds, and the - 660 to - 548 and - 179 to - 53 regions in the SeFAD2 promoter were implicated in ABA-responsive signaling. Theses observations indicate that an intron-mediated regulatory mechanism is involved in controlling not only the seed-specific expression of the SeFAD2 gene but also the expression of plant FAD2 genes, which are essential for the synthesis of polyunsaturated fatty acids.

show abstract

Section: Discussionmentioning

confidence: 97%

Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5′-UTR intron

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

“…In line with its specific expression in coffee seeds, inspection of the amplified CaSMP 5′-upstream region revealed the presence of cis-regulatory elements typically found in the promoters of seed-specific genes. Among them, the E-box has been shown to function in mediating high expression levels in seeds (Chandrasekharan et al 2003;Kim et al 2005). In this regard, validation of the cloned promoter fragment in transgenic tomato Micro-Tom lines corroborated promoter activity in the ovules of immature fruits, while no activity was observed in ripening fruits and in the other organs/tissues analyzed.…”

Section: Discussionmentioning

confidence: 85%

Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato

Quintero

Pinto

Barsalobres-Cavallari

et al. 2018

Plant Cell Rep

View full text Add to dashboard Cite

A seed maturation protein gene (CaSMP) from Coffea arabica is expressed in the endosperm of yellow/green fruits. The CaSMP promoter drives reporter expression in the seeds of immature tomato fruits. In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5'-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.

show abstract

“…2). According to previous reports, the E-and G-box elements in the SeFAD2, Cs-ACP1, and Cs-4PAD promoters were essential cis-elements for seed-specific expression (Kim et al 2005(Kim et al , 2006. Arabidopsis fatty acid elongase gene (FAE1), which catalyzes very-long-chain fatty acid biosynthesis, was specifically expressed in embryonic tissues.…”

Section: Discussionmentioning

confidence: 96%

“…Promoter regions at -117 and -312 of acyl carrier protein (Cs-ACP1) and D 4 -palmitoyl-acyl carrier protein desaturase (Cs-4PAD) genes, respectively, both of which are involved in biosynthesis of petroselinic acid in coriander (Coriandrum sativum) endosperms, include cis-regulatory elements such as the GCN4-like motif (GTCA), E-box, G-box, AACA motif, and Prolamin-box. These elements are required for endosperm-specific expression of Cs-ACP1 and Cs-4PAD genes (Kim et al 2005). In sesame, the region from -179 to -53 in the SeFAD2 promoter, including E-box (CANNTG), G-box (CACGTG), ABRE motif (ACGTGKC), G-box-like element (ACGT), RY repeat element (CATGCA), and Prolamin-box (AAAG), is essential for expression of the SeFAD2 gene in developing seeds (Kim et al 2006).…”

Section: Introductionmentioning

confidence: 99%

The SebHLH transcription factor mediates trans-activation of the SeFAD2 gene promoter through binding to E- and G-box elements

Kim

Shin

et al. 2007

Plant Mol Biol

Self Cite

View full text Add to dashboard Cite

Microsomal oleic acid desaturase (FAD2) catalyzes the first extra-plastidial desaturation in plants, converting oleic acid to linoleic acid, which is a major constituent in all cellular membranes as well as in seed storage oils. Seed-specific FAD2 (SeFAD2) produced 40% of linoleic acids in the total fatty acids of sesame (Sesamum indicum) seeds. The expression of SeFAD2 transcripts was spatially and temporally controlled during seed development. To investigate the regulatory mechanism controlling seed-specific SeFAD2 expression, we isolated a well-matched sequence homologous to the basic region/helix-loop-helix proteins by yeast one-hybrid screening and named it SebHLH. SebHLH transcripts were expressed in developing seeds and roots of sesame. SebHLH:GFP fusion protein localized in the nucleus. Recombinant SebHLH protein bound E-box (CANNTG) and G-box (CACGTG) elements in the region from -179 to -53 of the SeFAD2 gene promoter, and the external C and G nucleotides in the E- and G-box motifs were essential for SebHLH protein binding. The SebHLH gene, under the CaMV35S promoter, and the GUS reporter gene driven by E- and G-box motifs were co-expressed in developing sesame seeds and Arabidopsis transgenic leaves. This co-expression demonstrated that SebHLH protein mediates transactivation of the SeFAD2 gene promoter through binding to E- and G-box elements. E- or G-box elements frequently occur in the 5'-flanking region of genes that are involved in triacylglycerol biosynthesis and that exhibit seed-specific expression in Arabidopsis and other plants, suggesting that bHLH transcription factors play a key role in the transcriptional regulation of genes related to storage lipid biosynthesis and accumulation during seed development.

show abstract

Genomic structures and characterization of the 5′-flanking regions of acyl carrier protein and Δ4-palmitoyl-ACP desaturase genes from Coriandrum sativum

Cited by 10 publications

References 37 publications

Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5′-UTR intron

Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5′-UTR intron

Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato

The SebHLH transcription factor mediates trans-activation of the SeFAD2 gene promoter through binding to E- and G-box elements

Contact Info

Product

Resources

About