Genomic structure, expression pattern, and functional characterization of transcription factor E2F-2 from black tiger shrimp (Penaeus monodon)

Abstract

Transcription factor E2F-2 is a regulator of cell cycle. Researchers identified E2F-2 genes from yeasts to humans, but few reports investigated E2F-2 gene from black tiger shrimp. In the present study, we cloned E2F-2 gene from black tiger shrimp (Penaeus monodon). Full-length PmE2F-2 complementary DNA sequence measures 3,189 bp with an open reading frame of 1,371 bp. Complete PmE2F-2 genomic sequence (17,305 bp) of P. monodon contains nine exons, which are separated by eight introns. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that PmE2F-2 is highly expressed in hepatopancreas and ovaries of P. monodon. Highest PmE2F-2 expression levels were observed in stage III ovarian development of P. monodon. PmE2F-2 expression levels were significantly augmented in ovaries of P. monodon after 5-hydroxytryptamine injection and eyestalk ablation. RNA interference experiments were conducted to examine PmE2F-2, PmCDK2, and PmCyclin E expression profiles. PmE2F-2 was successfully knocked down in ovaries and hepatopancreas via double-stranded RNA (dsRNA)–E2F-2 injection. In the same organs, PmE2F-2 expression localization and level were investigated through in situ hybridization, which revealed consistent results with those of qRT-PCR. After dsRNA—E2F-2 injection, gonadosomatic index of shrimp was significantly lower than those following dsRNA—GFP and phosphate-buffered solution injections. Therefore, PmE2F-2 may be involved in ovarian maturation in P. monodon.

Identification of MicroRNAs and Their Target Genes Associated with Ovarian Development in Black Tiger Shrimp (Penaeus monodon) Using High-Throughput Sequencing

“…The black tiger shrimp ( P. monodon ) is a commercially important aquaculture species in South China and Southeast Asia 39 , 40 . At present, the numbers and quality of broodstock sourced from wild populations are in decline because of overfishing, causing difficulties for producers of farmed shrimp.…”

“…At present, the numbers and quality of broodstock sourced from wild populations are in decline because of overfishing, causing difficulties for producers of farmed shrimp. Although eyestalk ablation can induce broodstock ovarian maturation in P. monodon , this process can result in reduced egg quality and death of spawners 40 – 42 . Therefore, a better knowledge of the molecular controls of the reproductive system of this shrimp would be very useful to help resolve these problems.…”

Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia

“…Oogenesis predominantly includes primordial germ cell (PGC) formation and oocyte development, differentiation and maturation. These are controlled and regulated by relevant genes and signalling pathways [9]. As a key subject in developmental and reproductive biology, oogenesis has been extensively studied in mammals, insects and crustaceans [10][11][12].…”

Spatio-temporal patterns of ovarian development andVgRgene silencing reduced fecundity of parthenogeneticArtemia

“…In drosophila, oogenesis requires extensive interaction between somatic and germline stem cells, and these developmental events are accomplished in the ovaries (Fu et al, 2015b). Oogenesis mainly includes primordial germ cell (PGC) formation, oocyte development, differentiation and maturation, which were controlled and regulated by relevant genes and signaling pathways (Xie et al, 2017). As a hot subject in developmental biology and reproductive biology, oogenesis has been extensively studied in mammals, insects and a few crustaceans (Neaves and Baumann, 2011;Herawati et al, 2020;Zhang et al, 2022).…”