Genomic structure, expression, and functional characterization of the Fem-1 gene family in the redclaw crayfish, Cherax quadricarinatus

Zheng, Jianbo; Chen, Leran; Jia, Yongyi; Chi, Meili; Li, Fēi; Cheng, Sun; Liu, Shili; Liu, Yinuo; Gu, Zhenxin

doi:10.1016/j.ygcen.2021.113961

Cited by 11 publications

(3 citation statements)

References 42 publications

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“…Several previous studies have demonstrated that Fem-1c has a broad expression abundance throughout the entire gonadal developmental process; for example, the expression level of Lmfem-1c gradually increases during testis development in Locusta migratoria manilensis [9]. The highest expression of EsFem-1c exists in the early development stage of gonads in E. sinensis, which is similar to that found in Cherax quadricarinatus, Crassostrea gigas, and Penaeus vannamei [26,53,54], indicating that EsFem-1c is involved in the development of oocyte and spermatogenesis. In contrast, tissue distribution analysis in the present study revealed that the expression level of the EsFem-1c gene in the normal ovary was higher than that in the normal testis, consistent with the result in H. cumingii [2], indicating that EsFem-1c plays a critical role in female gonads.…”

Section: Discussionsupporting

confidence: 53%

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

Zhu

Feng

et al. 2023

Animals

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Feminization-1c (Fem-1c) is important for sex differentiation in the model organism Caenorhabditis elegans. In our previous study, the basic molecular characteristics of the Fem-1c gene (EsFem-1c) in Eriocheir sinensis (Henri Milne Edwards, 1854) were cloned to determine the relationship with sex differentiation. In this study, the genomic sequence of EsFem-1c contained five exons and four introns, with an exceptionally long 3′UTR sequence. The qRT-PCR results of EsFem-1c demonstrated lower tissue expression in the androgenic gland of the intersex crab than the normal male crab, implying that EsFem-1c plays a role in crab AG development. RNA interference experiments and morphological observations of juvenile and mature crabs indicated that EsFem-1c influences sexual development in E. sinensis. A dual-luciferase reporter assay disclosed that tcf-miR-315-5p effectively inhibits the translation of the EsFem-1c gene, influencing male development. An intriguing finding was that miRNA tcf-miR-307 could increase EsFem-1c expression by binding to the alternative splicing region with a length of 248 bp (ASR-248) in the 3′UTR sequence. The present research contributes to a better understanding of the molecular regulation mechanism of EsFem-1c and provides a resource for future studies of the miRNA-mediated regulation of sexual development and regulation in E. sinensis.

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Section: Discussionsupporting

confidence: 53%

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

Zhu

Feng

et al. 2023

Animals

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“…However, studies of Fem1 revealed that sexual reversal occurs after RNA interference with Fem1 in females (Gempe et al 2009). In Cherax quadricarinatus, Fem1 gene transcript levels were highest in ovary, and disruption of Fem1 decreased the expression level of vitellogenin (Zheng et al 2022). This suggests that Fem 1 is not only involved in male germ cell differentiation, but is also likely to be involved in regulating and stabilising female developmental pathways.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis reveals differential expression of sex-related genes in androgenic glands and ovaries of Macrobrachium rosenbergii

Li,

Zhou,

Xie

et al. 2024

Preprint

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Macrobrachium rosenbergii is an economically important crustacean worldwide. Based on the important role of the androgenic gland (AG) in sex determination in crustaceans, this study comparatively analysed the transcriptomes of AG and ovary tissues using Illumina sequencing technology. A total of 91,810 unigenes were generated from the six libraries after splicing and assembly, and 28,516 (31.1%) were annotated by BLASTx comparison with Nr and other databases. A total of 20,793 differentially expressed genes were identified in both tissues including 105 potentially related to sex determination and sex differentiation, of which 12 were ovary-specific and five were AG-specific. In addition, 14,794 simple sequence repeats (SSRs) and 289,882 single-nucleotide polymorphisms (SNPs) were identified. This study enriches the database of molecular genetic information available for M. rosenbergii, establishes a foundation for the analysis and application of molecular regulatory mechanisms of its reproductive traits, and advances the study of sex determination and sex differentiation in crustaceans. SSRs and SNPs mined from the transcriptomes may provide markers for functional genome research and the development of new technologies for molecular breeding.

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“…The overwhelming majority of crustaceans ( Eriocheir sinensis , Macrobrachium nipponense , Macrobrachium rosenbergii , et al) exhibited sexually dimorphic growth traits, and thus monosex cultivation contributed to increase yields and economic value (Song et al, 2015; Yaara et al, 2015). Recently, lots of important genes involved in sex differentiation and gonad development were identified, including Sxl/Tra2/dsx cascade signal pathways (Wang et al, 2020; Zheng et al, 2019), Dmrt family (Zhang & Qiu, 2010), Fem1 family (Song et al, 2015; Yaara et al, 2015; Zheng et al, 2022), CHH family (Chung et al, 2020), IAG (Li, Li, Sun, et al, 2012), Foxl2 (Li, Bai, et al, 2015), fruitless (Li et al, 2017), and so on. Crustaceans possessed a unique endocrine system, and nowadays its “eyestalk‐androgenic gland‐testis” endocrine axis had been confirmed to regulate male sexual differentiation (Guo et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the Ftz‐f1 gene in redclaw crayfish Cherax quadricarinatus and its potential role in ovarian development

Chen

Zheng

Jia

et al. 2022

Aquaculture Research

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Ftz‐f1 was a member of the NR5A‐family nuclear receptors, and previously predicted to be participated in the complex ovarian processes. In this study, we characterized the full‐length cDNA of Ftz‐f1 with an open reading frame of 1746 bp encoding 581 amino acids in the redclaw crayfish, Cherax quadricarinatus (CqFtz‐f1). Amino acid alignments and structural prediction uncovered that two highly conserved domain, DBD (DNA binding domain) and LBD (ligand binding domain) were identified, which shared with other members of nuclear receptors family. Tissues distribution showed that CqFtz‐f1 exhibited sexually dimorphic expression pattern with a higher transcript level in ovary. Staging of ovarian development was conducted, which to be divided into four different stages by external feature and histology analysis. The changes of GSI (Gonadosomatic index) were in accordance with the mRNA expression of CqFtz‐f1 during the reproductive cycle. In addition, both eyestalk ablation and β‐oestradiol (E2) injection significantly increased the expression levels of Ftz‐f1 and vg (vitellogenin). Furthermore, treatment with CqFtz‐f1 dsRNA injection generated distinct effects on the expression of vg, suggesting that CqFtz‐f1 might have positive effects on ovarian development by regulating vg expression in the redclaw crayfish.

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Genomic structure, expression, and functional characterization of the Fem-1 gene family in the redclaw crayfish, Cherax quadricarinatus

Cited by 11 publications

References 42 publications

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

Comparative transcriptome analysis reveals differential expression of sex-related genes in androgenic glands and ovaries of Macrobrachium rosenbergii

Molecular characterization of the Ftz‐f1 gene in redclaw crayfish Cherax quadricarinatus and its potential role in ovarian development

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