Genomic Strategies for Personalized Cancer Therapy

Dudek, Arkadiusz Z.; Baxstrom, Kate; Bharadwaj, Sushma; Blaes, Anne; Kulkarni, Amit; Lou, Emil; Nehru, Vijeyaluxmy Motilal; Rabinovich, Emma; Shergill, Ardaman; Viner, Maya

doi:10.1002/9781119432487.ch1

Cited by 2 publications

(1 citation statement)

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“…Additionally, studies have explored the correlation between specific morphological features and microsatellite instability (MSI) status in UCEC, shedding light on the presence of APC pathogenic mutations in this context [46,47]. Furthermore, investigations have also investigated germline pathogenic mutations in CDH1 gene and their implications in hereditary diffuse gastric cancer, which also has implications for UCEC given CDH1 mutations' broader significance [48,49].…”

Section: Drug Prediction Analysis Outcomesmentioning

confidence: 99%

Deciphering DNA repair gene mutational landscape in uterine corpus endometrial carcinoma patients using next generation sequencing

Lou

2024

Am J Cancer Res

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Uterine Corpus Endometrial Carcinoma (UCEC) is a significant health concern with a complex genetic landscape impacting disease susceptibility and progression. This study aimed to unravel the spectrum of DNA repair gene mutations in Pakistani UCEC patients through Next Generation Sequencing (NGS) and explore their potential functional consequences via downstream analyses. NGS analysis of genomic DNA from 30 UCEC patients was conducted to identify clinically significant pathogenic mutations in DNA repair genes. This analysis revealed mutations in 4 key DNA repair genes: BRCA1, BRCA2, APC, and CDH1. Kaplan-Meier (KM) analysis was employed to assess the prognostic value of these mutations on patient overall survival (OS) in UCEC. To delve into the functional impact of these mutations, we performed RT-qPCR, immunohistochemistry (IHC), and western blot analyses on the mutated UCEC samples compared to their non-mutated counterparts. These results unveiled the up-regulation in the expression of the mutated genes, suggesting a potential association between the identified mutations and enhanced gene activity. Additionally, targeted bisulfite sequencing analysis was utilized to evaluate DNA methylation patterns in the promoters of the mutated genes. Strikingly, hypomethylation in the promoters of BRCA1, BRCA2, APC, and CDH1 was observed in the mutated UCEC samples relative to the non-mutated, indicating the involvement of epigenetic mechanisms in the altered gene expression. In conclusion, this study offers insights into the genetic landscape of DNA repair gene mutations in Pakistani UCEC patients. The presence of pathogenic mutations in BRCA1, BRCA2, APC, and CDH1, coupled with their down-regulation and hypermethylation, suggests a convergence of genetic and epigenetic factors contributing to genomic instability in UCEC cells. These findings enhance our understanding of UCEC susceptibility and provide potential avenues for targeted therapeutic interventions in Pakistani UCEC patients.

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