Genomic Similarity of Taura Syndrome Virus (TSV) between Taiwan and Western Hemisphere Isolates.

Lien, Tzu-Wen; Hsiung, Hann-Chang; Huang, Chih-Cheng; Song, Yen-Ling

doi:10.3147/jsfp.37.71

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…The introduction of P. vannamei culture in Taiwan, China, Thailand and Indonesia also resulted in the spread of TS virus (TSV) to these regions within a relatively short period of time (Tu et al 1999, Yu & Song 2000, Nielsen et al 2005. Several genotypes or strains are now known to exist and the virus remains an important infectious disease in countries where P. vannamei is farmed (Erickson et al 2002, Lien et al 2002, Robles-Sikisaka et al 2002, Rosenberry 2002.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Taura syndrome virus (TSV) detection methods during chronic-phase infection in Penaeus vannamei

Poulos

Noble

Lightner³

2008

Dis. Aquat. Org.

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Methods to detect Taura syndrome virus (TSV) were assessed for their ability to detect the virus during chronic phase infection in the Pacific white shrimp Penaeus vannamei. In situ hybridization (ISH), immunohistochemistry (IHC) using monoclonal antibody 1A1, conventional RT-PCR and real-time quantitative (q)RT-PCR were compared using shrimp sampled over 60 wk following experimental TSV infection. Between Weeks 7 and 60, hematoxylin-eosin histology confirmed the presence of lymphoid organ spheroids (LOS) and an absence of lesions in the cuticular epithelium. ISH detected TSV in LOS over the duration of the study. IHC was generally less sensitive than ISH, and after Week 24, was often unable to confirm TSV infection. Detection of TSV by RT-PCR was highly dependent on sample source after Week 43, where viral RNA was detected in 12 of 14 hemolymph samples but only 5 of 16 pleopod samples. qRT-PCR detected TSV over the 60 wk in both hemolymph and pleopods, although RNA copy numbers in pleopods were consistently lower throughout the study. This study demonstrates that ISH and qRT-PCR are the most reliable methods for detecting TSV during late chronic phase infection. RT-PCR was also reliable if hemolymph was used as the sample source. KEY WORDS: Taura syndrome · TSV · RT-PCR · qRT-PCR · In situ hybridization · Immunohistochemistry Resale or republication not permitted without written consent of the publisherDis Aquat Org 82: [179][180][181][182][183][184][185] 2008 lasting ~5 d and a chronic phase that lingers in survivors of ecdysis. Shrimp with a chronic phase infection present no melanized lesions or abnormal behavior and histologically present normal cuticular epithelium. However, they display marked hypertrophy of the lymphoid organ (LO) with the presence of numerous LO spheroids (LOS) (Hasson et al. 1999b, Srisuvan et al. 2005.Over 48 wk following TSV infection of juvenile Penaeus vannamei, in situ hybridization (ISH) was able to detect TSV within the LOS up to Week 32, even though LOS remained evident thereafter up to Week 48 post-infection (pi) (Hasson et al. 1999b). RT-PCR and real-time quantitative (q)RT-PCR have been developed to detect TSV (Nunan et al. 1998) and the latter test was able to detect the virus in LO, gill and pleopod tissues in the chronic phase of the disease up to 23 wk pi when the study was terminated . A monoclonal antibody (1A1) generated to a TSV isolate from Hawaii (circa 1994) used for immunohistochemistry can detect the virus in fixed tissue sections but the reactivity is limited to related isolates (Poulos et al. 1999, Erickson et al. 2002. However, the utility of IHC has not been investigated during chronic phase infection.Studies to date have shown that accurate diagnosis of TSV is dependent upon the stage of infection and the nature of the isolate. The selection of diagnostic specimens and TSV detection methods becomes more important to diagnose the infection once disease signs abate as infection passes through the transition stage and the virus becomes sequestered...

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Section: Introductionmentioning

confidence: 99%

Comparison of Taura syndrome virus (TSV) detection methods during chronic-phase infection in Penaeus vannamei

Poulos

Noble

Lightner³

2008

Dis. Aquat. Org.

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“…Taura syndrome (TS), first recognized in Ecuador affecting Penaeus vannamei (Jimenez 1992, Lightner et al 1995, is now known to occur in most shrimpfarming countries in the Americas, and recently has spread to Taiwan, China, Thailand and Indonesia with the importation of P. vannamei for shrimp culture (Chien et al 1999, Tu et al 1999, Yu & Song 2000, Lien et al 2002. Concomitant with the rapid spread of disease among shrimp populations is the possibility of genetic variations occurring within the viral genome (Alday de Graindorge 2003).…”

Section: Introductionmentioning

confidence: 99%

Taura syndrome virus from Belize represents a unique variant

Erickson

Poulos

Tang³

et al. 2005

Dis. Aquat. Org.

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A Taura syndrome virus (TSV) isolate from cultured Penaeus vannamei grown in Belize, Central America was characterized and shown to be a unique isolate. Mortality rates in laboratory infections of specific pathogen-free (SPF) P. vannamei, reactivity of the virus with monoclonal antibody (MAb) 1A1 and phylogenetic analysis demonstrated that the Belize isolate (BLZ02TSV) is a new variant of TSV. The Hawaiian 1994 TSV isolate (HI94TSV, GenBank AF277675) was used as the reference isolate for these studies. Laboratory infections of SPF P. vannamei with BLZ02TSV demonstrated higher mortalities and earlier onset of mortalities compared to infections with HI94TSV. Shrimp tissues infected with BLZ02TSV reacted with a TSV-specific gene probe by in situ hybridization and were positive by RT-PCR using TSV diagnostic primers, thus indicating that the isolate was TSV. However, Western blot analysis and immunohistochemistry using MAb 1A1 demonstrated that BLZ02TSV did not react with the antibody, suggestive of changes in the VP1 region of the genome that codes for the polypeptide to which MAb 1A1 binds. Phylogenetic analysis of a 1.3 kbp fragment of the TSV VP1 capsid region revealed that BLZ02TSV represents a distinct group among more than 29 isolates of TSV studied thus far. This research demonstrates that BLZ02TSV is a unique isolate of TSV and reiterates a problem related to the use of MAb 1A1 for detection of TSV in clinical specimens. KEY WORDS: Taura syndrome virus · Belize · Monoclonal antibody 1A1Resale or republication not permitted without written consent of the publisher

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“…También, ha sido registrado hace algunos años en Taiwán y China, siendo el origen de la infección la especie L. vannamei procedente del hemisferio occidental (Lien et al 2002). Se han documentado infecciones en poblaciones silvestres de L. vannamei, L. stylirostris y L. setiferus, siendo en los estadios posteriores a postlarvas 12 y juveniles de langostinos, las infecciones más severas (Morales & Cuellar-Anjel 2008).…”

Section: Introductionunclassified

Prevalencia y distribución de los principales agentes etiológicos que afectan los langostinos silvestres en Tumbes, Perú

2011

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ResumenSe determinó la prevalencia y distribución de diferentes agentes patógenos en langostinos silvestres, en la zona de esteros de la Región Tumbes -Perú, entre marzo y diciembre de 2009. Los canales de marea considerados en este estudio fueron: Boca del Río Tumbes, El Alcalde, Jelí, El Bendito, Envidia, Soledad y Algarrobo. Se colectó un total de 1926 langostinos entre juveniles y pre-adultos de las especies Litopenaeus vannamei, L. stylirostris y Farfantepenaeus californiensis. Utilizando la técnica de la PCR, se detectó la presencia de los patógenos NHPB (0,62%), IHHNV (0,31%), BP (1,61%) y WSV (2,75%); no se encontró infección por TSV. Las tres especies en estudio fueron positivas a WSV y BP, presentándose la mayor prevalencia de infección por WSV (2,98%) en la especie L. stylirostris y por BP (2,66%) en L. vannamei. La NHPB fue detectada en las especies L. vannamei y L. stylirostris con 0,77% y 0,43% de prevalencia respectivamente. Se obtuvo una prevalencia de 0,52% para IHHNV en L. vannamei. Las más altas prevalencias de las infecciones por WSV, BP, NHPB e IHHNV se registraron en los canales de marea El Alcalde (10,79%), Algarrobo (4,51%), Envidia (2,26%) y Jelí (5,05%). Los datos señalan la presencia constante de diversos patógenos virales y bacterianos en diferentes especies de peneidos y su amplia distribución a lo largo del litoral tumbesino, lo que constituye un riesgo potencial para el desarrollo de la acuicultura en la región, y podría afectar las poblaciones naturales de langostinos.Palabras Clave: Prevalencia, WSV, IHHNV, BP, enfermedades langostinos. AbstractPrevalence and distribution of different pathogens of wild shrimp in the tidal channels of the Region TumbesPeru, during March to December 2009. The tidal channels considered in this study were: Boca del Rio Tumbes, El Alcalde, Jelí, El Bendito, Envidia, Soledad and Algarrobo. We collected a total of 1926 shrimps between juvenile and pre-adults of the species Litopenaeus vannamei, L. stylirostris y Farfantepenaeus californiensis. Using the PCR technique, detected the presence of pathogens NHPB (0.62%), IHHNV (0.31%), BP (1.61%) and WSV (2.75%); no infection was found by TSV. The three species studied were positive for WSV and BP showed the highest prevalence of WSV infection (2.98%) in the species L. stylirostris and BP (2.66%) in L. vannamei. The NHPB was detected in the species L. vannamei, L. stylirostris to 0.77% and 0.43% prevalence respectively. There was a prevalence of 0.52% for IHHNV in L. vannamei. The highest prevalence of infections WSV, BP, NHPB and IHHNV were recorded in the tidal channels El Alcalde (10.79%), Algarrobo (4.51%), Envidia (2.26%) and Jelí (5.05%) respectively. Data showed that the constant presence of various pathogens in different penaeid species and their wide distribution along the coast Tumbes, which could produce an impact on wild stocks of shrimp and being a potential risk for development of aquaculture in the region.

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Genomic Similarity of Taura Syndrome Virus (TSV) between Taiwan and Western Hemisphere Isolates.

Cited by 6 publications

References 17 publications

Comparison of Taura syndrome virus (TSV) detection methods during chronic-phase infection in Penaeus vannamei

Comparison of Taura syndrome virus (TSV) detection methods during chronic-phase infection in Penaeus vannamei

Taura syndrome virus from Belize represents a unique variant

Prevalencia y distribución de los principales agentes etiológicos que afectan los langostinos silvestres en Tumbes, Perú

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