Genomic sequencing of Thinopyrum elongatum chromosome arm 7EL, carrying fusarium head blight resistance, and characterization of its impact on the transcriptome of the introgressed line CS-7EL

Konkin, David; Hsueh, Ya-Chih; Kirzinger, Morgan W. B.; Kubaláková, Marie; Haldar, Aparna; Balcerzak, Margaret; Han, Fangpu; Fedak, George; Doležel, Jaroslav; Sharpe, Andrew; Ouellet, Thérèse

doi:10.1186/s12864-022-08433-8

Cited by 13 publications

(12 citation statements)

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“…The same results were shown in wheat lines with fragments 7EL chromosome from Th. elongatum [16]. Transcriptomic analysis of six disomic substitution lines of Triticum aestivum/Thinopyrum elongatum in comparison with parental genotypes showed that the change of expression affected 57.43-69.22 % of Thinopyrum genes and only 2.19-8.97 % of wheat genes.…”

Section: Facts Of the Variability Of The Recipient Genome Of Hybrid O...mentioning

confidence: 99%

Significance of introgression hybridization for extension of genetic variability in recipient genome

Аntonyuk

Shpylchyn

Martynenko

et al. 2022

NRPBE

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Alien chromatin (introgressions) can be incorporated into the wheat genome either through recombination or through translocation. Lines of introgressive origin may not contain identified alien chromatin, although they appear to be changed relative to the original wheat sample by some characteristics that are registered at different levels of phenotype assessment. It has been shown numerous facts of the variability of the recipient genome of hybrid origin. They can be caused by irregular behavior of chromosomes in mitoses of somatic cells with hybrid genome, and DNA changes. Wide phenotypic variability was observed among the progeny of hybrid genomes up to appearance of phenotypic novelties. The study of DNA profiles using several methods revealed the presence of numerous genetic and epigenetic changes, including deletions, changes in gene expression, changes in the methylation pattern, and activation of retrotransposons in the recipient genome. When these changes were not directly related to alien fragments, they are considered as possible mechanisms of extension of genetic variability intra genome of hybrid origin. Transcriptomic analysis of lines of hybrid origin and parental plants using the RNA-seq analyses showed that the change of expression affected a different number of genes. The change in the expression of alien genes occurred in the direction of down-regulation, upregulation was typical for wheat genes. Phenotypic variability during introgressive hybridization can be accompanied by attenuation of the expression of an alien gene when it enters the recipient genome. That may be a consequence of the interaction between the alien gene and the genetic environment of the host genome. Today there is a lot of evidence that these mechanisms are epigenetic, which include DNA methylation, histone modification, the involvement of small RNAs in the regulation of gene expression, and the involvement of transposons. Moreover, none of these mechanisms act independently, but are a component of events, the results of which can be a change in gene expression and the appearance of a new phenotype that is not related to the expression of an alien gene.

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Section: Facts Of the Variability Of The Recipient Genome Of Hybrid O...mentioning

confidence: 99%

Significance of introgression hybridization for extension of genetic variability in recipient genome

Аntonyuk

Shpylchyn

Martynenko

et al. 2022

NRPBE

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“…ponticum QTL and the Th. elongatum locus, here referred to as Fhb7E [ 38 , 63 , 64 , 65 ]. Furthermore, Fhb7el 2 and Fhb7E confer a largely comparable phenotype when transferred into wheat, corresponding to an exceptionally high resistance to FHB spreading along the spike beyond the Fg inoculation point, and in addition an extremely low DON content in mature grains of recipient bread and durum wheat [ 60 , 62 , 66 ].…”

Section: Introductionmentioning

confidence: 99%

“…elongatum 7EL arm added to bread wheat cv. Chinese Spring (CS), i.e., CS-7EL [ 65 ]. This 7EL arm, originally shown to confer high FHB resistance to CS wheat [ 68 ], belongs to the Dvorak74 Th.…”

Section: Introductionmentioning

confidence: 99%

“…elongatum accession, distinct from the D-3458 of Wang et al [ 38 ]. Despite some structural differences between the respective GST sequences, a preliminary expression analysis revealed the Dvorak74- GST gene to be among the few 7EL-specific transcripts strongly upregulated by Fg infection [ 65 ]. The presence of a GST gene showing high homology or complete identity with the 7el 2 Th.…”

Section: Introductionmentioning

confidence: 99%

“…This evidence led the authors to exclude the 7EL GST gene, and so its 7el 2 homolog ( Fhb7 ) was found to be the pivotal gene in conferring FHB resistance to wheat [ 63 , 64 ]. In fact, irrespective of their response to Fg infection, several other transcripts with the potential to contribute to FHB resistance were detected in the 7EL distal region [ 63 , 65 ]. Apart from 7EL-specific genes/products, comparative analyses at the transcriptional, biochemical and microscopic levels, focused on the rachis tissue of CS vs. CS-7EL, identified candidates for more active constitutive or induced mechanisms of resistance in the presence of 7EL involving carbon and phenylpropanoid metabolism [ 68 , 69 ], fortification of the plant cell wall [ 69 , 70 ], defence response signalling and fungal cell wall degradation [ 65 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Untargeted Metabolomics Reveals a Multi-Faceted Resistance Response to Fusarium Head Blight Mediated by the Thinopyrum elongatum Fhb7E Locus Transferred via Chromosome Engineering into Wheat

Fanelli

Kuzmanović

Giovenali

et al. 2023

Cells

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The Thinopyrum elongatum Fhb7E locus has been proven to confer outstanding resistance to Fusarium Head Blight (FHB) when transferred into wheat, minimizing yield loss and mycotoxin accumulation in grains. Despite their biological relevance and breeding implications, the molecular mechanisms underlying the resistant phenotype associated with Fhb7E have not been fully uncovered. To gain a broader understanding of processes involved in this complex plant–pathogen interaction, we analysed via untargeted metabolomics durum wheat (DW) rachises and grains upon spike inoculation with Fusarium graminearum (Fg) and water. The employment of DW near-isogenic recombinant lines carrying or lacking the Th. elongatum chromosome 7E region including Fhb7E on their 7AL arm, allowed clear-cut distinction between differentially accumulated disease-related metabolites. Besides confirming the rachis as key site of the main metabolic shift in plant response to FHB, and the upregulation of defence pathways (aromatic amino acid, phenylpropanoid, terpenoid) leading to antioxidants and lignin accumulation, novel insights were revealed. Fhb7E conferred constitutive and early-induced defence response, in which specific importance of polyamine biosynthesis, glutathione and vitamin B6 metabolisms, along with presence of multiple routes for deoxynivalenol detoxification, was highlighted. The results suggested Fhb7E to correspond to a compound locus, triggering a multi-faceted plant response to Fg, effectively limiting Fg growth and mycotoxin production.

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Wheat Chromosomal Resources and Their Role in Wheat Research

Šimková,

Cápal,

Doležel

2023

Compendium of Plant Genomes

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Bread wheat (Triticum aestivum L.) is grown on more area of land than any other crop, and its global significance is challenged only by rice. Despite the socioeconomic importance, the wheat genome research was lagging behind other crops for a long time. It was mainly a high complexity of the genome, polyploidy and a high content of repetitive elements that were laying obstacles to a thorough genome analysis, gene cloning and genome sequencing. Solution to these problems came in the beginning of the new millennium with the emergence of chromosome genomics—a new approach to studying complex genomes after dissecting them into smaller parts—single chromosomes or their arms. This lossless complexity reduction, enabled by flow-cytometric chromosome sorting, reduced the time and cost of the experiment and simplified downstream analyses. Since the approach overcomes difficulties due to sequence redundancy and the presence of homoeologous subgenomes, the chromosomal genomics was adopted by the International Wheat Genome Sequencing Consortium (IWGSC) as the major strategy to sequence bread wheat genome. The dissection of the wheat genome into single chromosomes enabled the generation of chromosome survey sequences and stimulated international collaboration on producing a reference-quality assembly by the clone-by-clone approach. In parallel, the chromosomal resources were used for marker development, targeted mapping and gene cloning. The most comprehensive approaches to gene cloning, such as MutChromSeq and assembly via long-range linkage, found their use even in the post-sequencing era. The chapter provides a two-decade retrospective of chromosome genomics applied in bread wheat and its relatives and reports on the chromosomal resources generated and their applications.

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Genomic sequencing of Thinopyrum elongatum chromosome arm 7EL, carrying fusarium head blight resistance, and characterization of its impact on the transcriptome of the introgressed line CS-7EL

Cited by 13 publications

References 125 publications

Significance of introgression hybridization for extension of genetic variability in recipient genome

Significance of introgression hybridization for extension of genetic variability in recipient genome

Untargeted Metabolomics Reveals a Multi-Faceted Resistance Response to Fusarium Head Blight Mediated by the Thinopyrum elongatum Fhb7E Locus Transferred via Chromosome Engineering into Wheat

Wheat Chromosomal Resources and Their Role in Wheat Research

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