Genomic sequencing.

Church, George M.; Gilbert, Walter

doi:10.1073/pnas.81.7.1991

Cited by 7,564 publications

(2,733 citation statements)

References 36 publications

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“…A 198-bp oligonucleotide probe (58 nucleotides [nts] of intron 2 followed by codons for aa 87–130 of both proteins) was synthesized corresponding to this sequence and used to probe a Drosophila genomic P1 library filter (Genome Systems, Inc.). All DNA hybridizations were performed at 60°C according to Church and Gilbert 1984. This probe identified two P1s, DSO5055 and DSO3675, both of which map to chromosome 2L(32D-E).…”

Section: Methodsmentioning

confidence: 99%

CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila

Yucel

Marszalek

McIntosh

et al. 2000

The Journal of Cell Biology

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CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element–mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.

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Section: Methodsmentioning

confidence: 99%

CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila

Yucel

Marszalek

McIntosh

et al. 2000

The Journal of Cell Biology

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“…The PR-1, -2, and -5 cDNA probes were described by Uknes et al (1992). Hybridization and washing conditions were as described (Church and Gilbert, 1984). Relative amounts of transcript were determined by probing stripped filter blots with the constitutively expressed ~-tubulin cDNA to normalize loading and radio-decay was detected using a Phosphorlmager (Molecular Dynamics, Sunnyvale, CA) according to the manufacturer's instructions.…”

Section: Rna Extraction and Analysismentioning

confidence: 99%

Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway

et al. 1996

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“…The RNA hybridizing with cDNA probes was visualized by autoradiography using an intensifying screen at -80°C (Church and Gilbert, 1984). Resulting bands on the autoradiograph were analyzed using a phosphorimager S 1 densitometer.…”

Section: H M Schipper Et Almentioning

confidence: 99%

Mitochondrial Iron Sequestration in Dopamine‐Challenged Astroglia: Role of Heme Oxygenase‐1 and the Permeability Transition Pore

Schipper

Bernier

Mehindate

et al. 1999

Journal of Neurochemistry

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Abstract:Little is currently known concerning the mechanisms responsible for the excessive deposition of redox-active iron in the substantia nigra of subjects with Parkinson's disease (PD). In the present study, we demonstrate that dopamine promotes the selective sequestration of non-transferrin-derived iron by the mitochondrial compartment of cultured rat astroglia and that the mechanism underlying this novel dopamine effect is oxidative in nature. We also provide evidence that up-regulation of the stress protein heme oxygenase-1 (HO-1) is both necessary and sufficient for mitochondrial iron trapping in dopamine-challenged astroglia. Finally, we show that opening of the mitochondrial transition pore (MTP) mediates the influx of non-transferrin-derived iron into mitochondria of dopamine-stimulated and HO-1 -transfected astroglia. Our findings provide an explanation for the pathological iron sequestration, mitochondrial insufficiency, and amplification of oxidative injury reported in the brains of PD subjects. Pharmacological blockade of transition metal trapping by "stressed" astroglial mitochondria (e.g., using HO-1 inhibitors or modulators of the MTP) may afford effective neuroprotection in patients with PD and other neurological afflictions.

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Genomic sequencing.

Cited by 7,564 publications

References 36 publications

CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila

CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila

Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway

Mitochondrial Iron Sequestration in Dopamine‐Challenged Astroglia: Role of Heme Oxygenase‐1 and the Permeability Transition Pore

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