Genomic Sequence of Campylobacter jejuni subsp.
            <i>jejuni</i>
            HS:19 Penner Serotype Reference Strain RM3420

Parker, Craig T.; Huynh, Steven; Heikema, Astrid P.

doi:10.1128/genomea.01701-16

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…This reductase plays a key role in dissimilatory sulphite reduction but misses an N-terminal signal sequence in C. jejuni HS:19 that is critical for its periplasmic localization and function. In agreement, gene CjjRM3420_0034 was annotated as a pseudogene in strain RM3420 [ 13 ]. Based on the presence of multiple mutations, we concluded that the mcc -like cluster needed for sulphite reduction is probably not functional in C. jejuni HS:19 strains.…”

Section: Resultsmentioning

confidence: 94%

“…Five uncomplicated enteritis-related strains originate from Dutch patients, and one strain was isolated from the stool of a patient with enteritis from Canada. The complete genome sequence of this Canadian strain, also known as the HS:19 Penner reference strain, was published previously [ 13 ]. The food-related strain was isolated from packed chicken meat in the USA, and its full genome was also published previously [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

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Biomolecule sulphation and novel methylations related to Guillain-Barré syndrome-associated Campylobacter jejuni serotype HS:19

et al. 2021

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Campylobacter jejuni strains that produce sialylated lipooligosaccharides (LOS) can cause the immune-mediated disease Guillain-Barré syndrome (GBS). The risk of GBS after infection with C. jejuni Penner serotype HS:19 is estimated to be at least six times higher than the average risk. Aside from LOS biosynthesis genes, genomic characteristics that promote an increased risk for GBS following C. jejuni HS:19 infection, remain uncharacterized. We hypothesized that strains with the HS:19 serotype have unique genomic features that explain the increased risk for GBS. We performed genome sequencing, alignments, single nucleotide polymorphisms' analysis and methylome characterization on a subset, and pan-genome analysis on a large number of genomes to compare HS:19 with non-HS:19 C. jejuni genome sequences. Comparison of 36 C. jejuni HS:19 with 874 C. jejuni non-HS:19 genome sequences led to the identification of three single genes and ten clusters containing contiguous genes that were significantly associated with C. jejuni HS:19. One gene cluster of seven genes, localized downstream of the capsular biosynthesis locus, was related to sulphation of biomolecules. This cluster also encoded the campylobacter sialyl transferase Cst-I. Interestingly, sulphated bacterial biomolecules such as polysaccharides can promote immune responses and, therefore, (in the presence of sialic acid) may play a role in the development of GBS. Additional gene clusters included those involved in persistence-mediated pathogenicity and gene clusters involved in restriction-modification systems. Furthermore, characterization of methylomes of two HS:19 strains exhibited novel methylation patterns (5′-CATG-3 and 5′-m6AGTNNNNNNRTTG-3) that could differentially effect gene-expression patterns of C. jejuni HS:19 strains. Our study provides novel insight into specific genetic features and possible virulence factors of C. jejuni associated with the HS:19 serotype that may explain the increased risk of GBS.

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Section: Resultsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Biomolecule sulphation and novel methylations related to Guillain-Barré syndrome-associated Campylobacter jejuni serotype HS:19

et al. 2021

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“…This strain has an increased resistance to inactivation by high hydrostatic pressure compared to the other C. jejuni strains ( Gunther et al, 2015 ). Strains RM3420 and RM1285 both belong to serotype HS:19, which is believed to be a unique clone that is associated with GBS and gastroenteritis ( Misawa et al, 2001 ; Parker et al, 2017 ). The stains ATCC33560 and FDAARGOS_295 are quality control strains used for the antimicrobial susceptibility testing of Campylobacter spp.…”

Section: Resultsmentioning

confidence: 99%

Multi-Omics Approach Reveals the Potential Core Vaccine Targets for the Emerging Foodborne Pathogen Campylobacter jejuni

Cao

Cai

et al. 2021

Front. Microbiol.

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Campylobacter jejuni is a leading cause of bacterial gastroenteritis in humans around the world. The emergence of bacterial resistance is becoming more serious; therefore, development of new vaccines is considered to be an alternative strategy against drug-resistant pathogen. In this study, we investigated the pangenome of 173 C. jejuni strains and analyzed the phylogenesis and the virulence factor genes. In order to acquire a high-quality pangenome, genomic relatedness was firstly performed with average nucleotide identity (ANI) analyses, and an open pangenome of 8,041 gene families was obtained with the correct taxonomy genomes. Subsequently, the virulence property of the core genome was analyzed and 145 core virulence factor (VF) genes were obtained. Upon functional genomics and immunological analyses, five core VF proteins with high antigenicity were selected as potential core vaccine targets for humans. Furthermore, functional annotations indicated that these proteins are involved in important molecular functions and biological processes, such as adhesion, regulation, and secretion. In addition, transcriptome analysis in human cells and pig intestinal loop proved that these vaccine target genes are important in the virulence of C. jejuni in different hosts. Comprehensive pangenome and relevant animal experiments will facilitate discovering the potential core vaccine targets with improved efficiency in reverse vaccinology. Likewise, this study provided some insights into the genetic polymorphism and phylogeny of C. jejuni and discovered potential vaccine candidates for humans. Prospective development of new vaccines using the targets will be an alternative to the use of antibiotics and prevent the development of multidrug-resistant C. jejuni in humans and even other animals.

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Why 'Permissioned' and 'Private' are not Blockchains

Konashevych¹

2019

SSRN Journal

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Genomic Sequence of Campylobacter jejuni subsp. jejuni HS:19 Penner Serotype Reference Strain RM3420

Cited by 3 publications

References 19 publications

Biomolecule sulphation and novel methylations related to Guillain-Barré syndrome-associated Campylobacter jejuni serotype HS:19

Biomolecule sulphation and novel methylations related to Guillain-Barré syndrome-associated Campylobacter jejuni serotype HS:19

Multi-Omics Approach Reveals the Potential Core Vaccine Targets for the Emerging Foodborne Pathogen Campylobacter jejuni

Why 'Permissioned' and 'Private' are not Blockchains

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