Genomic RNAi screening in Drosophila S2 cells: what have we learned about host–pathogen interactions?

Cherry, Sara

doi:10.1016/j.mib.2008.05.007

Cited by 54 publications

(43 citation statements)

References 59 publications

(65 reference statements)

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“…The subtle measures of attenuation observed with the ysa mutants in mice, combined with the complication of conflicting temperature requirements for Ysa T3SS function and mammalian tissue culture models, have presented challenges to studying the specific role that this system plays in pathogenesis. Drosophila S2 cells have proven to be an excellent model for investigating host-pathogen interactions (40). In addition, S2 cells are cultured at a temperature that is consistent with Ysa gene expression and secretion.…”

Section: Discussionmentioning

confidence: 99%

A Phenotype at Last: Essential Role for the Yersinia enterocolitica Ysa Type III Secretion System in a Drosophila melanogaster S2 Cell Model

et al. 2013

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The highly pathogenic Yersinia enterocolitica strains have a chromosomally encoded type III secretion system (T3SS) that is expressed and functional in vitro only when the bacteria are cultured at 26°C. Mutations that render this system nonfunctional are slightly attenuated in the mouse model of infection only following an oral inoculation and only at early time points postinfection. The discrepancy between the temperature required for the Ysa gene expression and the physiological temperature required for mammalian model systems has made defining the role of this T3SS challenging. Therefore, we explored the use of Drosophila S2 cells as a model system for studying Ysa function. We show here that Y. enterocolitica is capable of infecting S2 cells and replicating intracellularly to high levels, an unusual feature of this pathogen. Importantly, we show that the Ysa T3SS is required for robust intracellular replication. A secretion-deficient mutant lacking the secretin gene, ysaC, is defective in replication within S2 cells, marking the first demonstration of a pronounced Ysa-dependent virulence phenotype. Establishment of S2 cells as a model for Y. enterocolitica infection provides a versatile tool to elucidate the role of the Ysa T3SS in the life cycle of this gastrointestinal pathogen.

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Section: Discussionmentioning

confidence: 99%

A Phenotype at Last: Essential Role for the Yersinia enterocolitica Ysa Type III Secretion System in a Drosophila melanogaster S2 Cell Model

et al. 2013

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“…Cell lines of animal origin have often been used to study interactions between pathogenic organisms and host cells ex vivo (Cherry, 2008;Veiga & Cossart, 2006). These cellular models are currently used to study the mechanisms of cell infection by a wide variety of animal pathogens, including mycoplasmas (Burnett et al, 2006;Drasbek et al, 2007;Fleury et al, 2002;Giron et al, 1996;Svenstrup et al, 2002;Winner et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Infection of the Circulifer haematoceps cell line Ciha-1 by Spiroplasma citri: the non-insect-transmissible strain 44 is impaired in invasion

et al. 2010

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Successful transmission of Spiroplasma citri by its leafhopper vector requires a specific interaction between the spiroplasma surface and the insect cells. With the aim of studying these interactions at the cellular and molecular levels, a cell line, named Ciha-1, was established using embryonic tissues from the eggs of the S. citri natural vector Circulifer haematoceps. This is the first report, to our knowledge, of a cell line for this leafhopper species and of its successful infection by the insect-transmissible strain S. citri GII3. Adherence of the spiroplasmas to the cultured Ciha-1 cells was studied by c.f.u. counts and by electron microscopy. Entry of the spiroplasmas into the insect cells was analysed quantitatively by gentamicin protection assays and qualitatively by double immunofluorescence microscopy. Spiroplasmas were detected within the cell cytoplasm as early as 1 h after inoculation and survived at least 2 days inside the cells. Comparing the insect-transmissible GII3 and non-insect-transmissible 44 strains revealed that adherence to and entry into Ciha-1 cells of S. citri 44 were significantly less efficient than those of S. citri GII3.

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“…The nature of some of the cellular factors is now emerging from various 'omics technologies as well as genome-wide RNA interference screens [8,9] [10,11]. In many instances, these host factors control specific organelle properties, such as ion homeostasis, subcellular transport, formation and maintenance of membrane domains and membrane sorting processes [12].…”

Section: Discussionmentioning

confidence: 99%

Too smart to fail—how viruses exploit the complexity of host cells during entry

Greber

Cosset

2011

Current Opinion in Virology

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Genomic RNAi screening in Drosophila S2 cells: what have we learned about host–pathogen interactions?

Cited by 54 publications

References 59 publications

A Phenotype at Last: Essential Role for the Yersinia enterocolitica Ysa Type III Secretion System in a Drosophila melanogaster S2 Cell Model

A Phenotype at Last: Essential Role for the Yersinia enterocolitica Ysa Type III Secretion System in a Drosophila melanogaster S2 Cell Model

Infection of the Circulifer haematoceps cell line Ciha-1 by Spiroplasma citri: the non-insect-transmissible strain 44 is impaired in invasion

Too smart to fail—how viruses exploit the complexity of host cells during entry

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