Genomic responses of the bovine mammary gland and epithelial cells to acute LPS challenge

Kerr, David E.; Latshaw, M.; Pareek, Ravi; Zheng, Jingwen; Bond, Jeffery

doi:10.1016/j.vetimm.2008.10.013

Cited by 2 publications

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“…Chemokine (C‐X‐C motif) ligand 12a was downregulated by the LPS in P. promelas . CXCL chemokines play an important role in leucocyte chemotaxis and are stimulated and upregulated by LPS in mammals (Kerr et al , 2009). Fish CXCL12, however, was constitutively expressed at the same level in both immune‐ and non‐immune‐related tissues in the channel catfish Ictalurus punctatus (Rafinesque 1818) and could not be induced by Edwardsiella ictaluri challenge (Baoprasertkul et al , 2005) or by LPS and phorbol myristate acetate applied to the anterior kidney of common carp Cyprinus carpio L. 1758 (Huising et al , 2004).…”

Section: Discussionmentioning

confidence: 99%

Immunological stimuli change expression of genes and neutrophil function in fathead minnow Pimephales promelas Rafinesque

Jovanović

Goetz

et al. 2011

Journal of Fish Biology

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Fathead minnows Pimephales promelas were exposed to lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid [poly(I:C)] to observe immunological responses during simulated bacterial and viral challenge at the level of gene expression and granulocyte function. Complementary DNA libraries were created from LPS- and poly(I:C)-treated fish and c. 5000 expressed sequence tags (ESTs) were sequenced. The ESTs were subjected to BLASTx analysis and 1500 genes were annotated, grouped by function and 20 immune genes were selected for expression studies by real-time PCR. Lipopolysaccharide treatment significantly downregulated expression of interferon regulatory factor 2 binding protein 1 (nine-fold), Chemokine (C-X-C motif) ligand 12a (three-fold) and TNF-related apoptosis-inducing ligand, TRAIL (two-fold). In poly(I:C)-treated fish, a significant upregulation was observed for IFN-inducible and antiviral proteins belonging to the family of Mx proteins (73-fold) and chemokine CCL-C5a (28-fold). Blood neutrophil count was significantly increased in poly(I:C)-treated fish at 24 and 48 h post-injection. Neutrophil extracellular trap release and respiratory burst of kidney granulocytes were suppressed in poly(I:C)-treated fish, while degranulation of primary granules was not affected significantly by the treatment. The changes in gene expression and neutrophil function in P. promelas exposed to LPS and poly(I:C) support the use of this species as an alternative model for studies of pathogen effects on the innate immune system of fishes.

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Section: Discussionmentioning

confidence: 99%

Immunological stimuli change expression of genes and neutrophil function in fathead minnow Pimephales promelas Rafinesque

Jovanović

Goetz

et al. 2011

Journal of Fish Biology

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show abstract

“…CXCL chemokines play an important role in leukocyte chemotaxis and are stimulated and upregulated by LPS in mammals (Kerr et al, 2009). However, fish CXCL12 was constitutively expressed at the same level in both immune and non-immune related tissues in the channel catfish, Ictalurus punctatus Rafinesque 1818, and could not be induced by Edwardsiella ictaluri challenge (Baoprasertkul et al, 2005), or by LPS and phorbol myristate acetate applied to the anterior kidney of common carp Cyprinus carpio L.…”

Section: Discussionmentioning

confidence: 99%

Immunotoxicology of titanium dioxide and hydroxylated fullerenes engineered nanoparticles in fish models

Jovanović

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Genomic responses of the bovine mammary gland and epithelial cells to acute LPS challenge

Cited by 2 publications

References 0 publications

Immunological stimuli change expression of genes and neutrophil function in fathead minnow Pimephales promelas Rafinesque

Immunological stimuli change expression of genes and neutrophil function in fathead minnow Pimephales promelas Rafinesque

Immunotoxicology of titanium dioxide and hydroxylated fullerenes engineered nanoparticles in fish models

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