Abstract:Background: Voles of the genus Microtus are important research organisms, yet genomic resources in the genus are lacking. Providing such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. Findings: We sequenced and assembled nuclear genomes from two subspecies of water vole (Microtus richardsoni) and from the montane vole (Microtus montanus). The water vole genomes were sequenced with Illumina and 10X Chromium plus Illumina sequencing, resulting in assemblies wit… Show more
“…The contaminating sequences were likely to be nuclear sequences of mitochondrial origin ( numts ). The presence of numts in mtDNA assemblies was previously reported for various Microtus species (Barbosa et al, 2018 ; Duckett et al, 2021 ; Triant & DeWoody, 2008 ), and their presence are especially expected in the case of the assemblies based on mtDNA‐enriched libraries. Therefore, we called consensus sequences only for mtDNA fragments where numts were not present, and this was either whole mtDNA, ca.…”
This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
“…The contaminating sequences were likely to be nuclear sequences of mitochondrial origin ( numts ). The presence of numts in mtDNA assemblies was previously reported for various Microtus species (Barbosa et al, 2018 ; Duckett et al, 2021 ; Triant & DeWoody, 2008 ), and their presence are especially expected in the case of the assemblies based on mtDNA‐enriched libraries. Therefore, we called consensus sequences only for mtDNA fragments where numts were not present, and this was either whole mtDNA, ca.…”
This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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