Genomic profiling of Pasteurella multocida isolated from feedlot cases of bovine respiratory disease

Alhamami, Tamara; Chowdhury, Piklu Roy; Venter, Henrietta; Veltman, Tania; Truswell, Alec; Abraham, Sam; Sapula, Sylvia A.; Carr, Mandi; Djordjevic, Steven P.; Trott, Darren J.

doi:10.1016/j.vetmic.2023.109773

Cited by 5 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As expected on the basis of previous data [5,13,15,22], all P. multocida isolates belonged to genotype A: L3, corroborating the association of this genotype with clinical cases of BRD, as well as the limited capacity of capsular LPS genotyping to show the genetic diversity of P. multocida isolates and to differentiate between related strains [5,8]. P. multocida isolates from BRD cases and those from healthy animals were further characterized by PFGE due to its high discriminatory power [32].…”

Section: Discussionsupporting

confidence: 84%

“…Whole-genome sequencing (WGS) has been used for the genotyping of BRD-associated P. multocida isolates [15]. Thus, a subset of 14 P. multocida isolates representative of pulsotypes identified in isolates from both clinically BRD-affected calves and apparently healthy calves were selected for WGS, and in silico analysis of multilocus sequence types, virulenceassociated genes, and antimicrobial resistance genes was carried out.…”

Section: Discussionmentioning

confidence: 99%

“…Sequence types ST79 and ST13 also seem to be the most prevalent among respiratory isolates from healthy cattle [6]. Whole-genome sequencing with phylogenetic analysis of P. multocida genomes identified ST79 as the most dominant clonal lineage in BRDassociated isolates worldwide and suggested that ST79 shows host specificity to cattle but genomic heterogeneity [8,14,15]. Using PFGE, it was found that some closely related clones (pulsotypes) were consistently associated with clinical cases of BRD [5].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Calderón Bernal,

Serna,

García Muñoz

et al. 2023

Animals

View full text Add to dashboard Cite

The aim of this study was to investigate the possible genotypic differences between commensal Pasteurella multocida isolates from apparently healthy animals (AHA) at the time of entry to feedlots and those from BRD-affected animals (BRD-AA). A total of 20 batches of beef calves in seven feedlots were followed-up during the fattening period. P. multocida was isolated from 28.1% of AHA and 22.9% of BRD-AA. All isolates belonged to the A: L3 genotype. Most isolates from clinical cases (81.0%) grouped into a PFGE cluster were significantly associated with BRD cases (OR, 24.9; 95% CI, 6.4–96.2). The whole genomes of 14 isolates representative of the pulsotypes most frequently detected in BRD-AA and AHA were sequenced and compared with 53 bovine genomes belonging to the identified ST13, ST79, and ST80 genotypes for a global comparison. No differences were found in the virulence-associated gene content between sequence types (STs) globally or between BRD-AA and AHA isolates in this study. Significantly, ST79 isolates harbored ARGs, conferring resistance to different antimicrobials, including macrolides and tetracyclines, which are commonly used for the treatment of BRD. Two Spanish ST79 isolates carried an ICE highly similar to ICE Tn7407, which was recently detected in Germany, suggesting that ST79 P. multocida isolates in Europe and North America may be associated with different ICEs.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Calderón Bernal,

Serna,

García Muñoz

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

“…Beyond the N-terminal end, the peptide sequences are 99.6% identical with a single serine to methionine conversion (Figure S2 ) closer to the C-terminal end. Alignment analysis of estT gene with a collection of P. multocida genomes 17 , 18 led to the identification of five in-house (BRD17-035, 18BRD-001, 19BRD-032, 19BRD-042 and 19BRD-057) and four RefSeq genomes (GCF_001930605.1, GCF_002859385.1, GCF_002859485.1 and GCF_003261475.1), which host the homologue.…”

Section: Resultsmentioning

confidence: 99%

“…Our genomic surveillance study on Australian BRD-associated P. multocida indicated that ST394 was linked to the emergence of resistance in BRD pathogens. 17 In 2022, we published the reference genome sequence of P. multocida 17BRD-035 (CP082272.1) representing ST394. 18 Isolate 17BRD-035 was resistant to tetracycline, penicillin and tilmicosin but not tulathromycin.…”

Section: Introductionmentioning

confidence: 99%

Identification and evolution of ICE-PmuST394: a novel integrative conjugative element in Pasteurella multocida ST394

Roy Chowdhury,

Alhamami,

Venter

et al. 2024

Journal of Antimicrobial Chemotherapy

Self Cite

View full text Add to dashboard Cite

Background The emergence of macrolide and tetracycline resistance within Pasteurella multocida isolated from feedlot cattle and the dominance of ST394 in Australia was reported recently. Objectives To establish the genetic context of the resistance genes in P. multocida 17BRD-035, the ST394 reference genome, and conduct a molecular risk assessment of their ability to disperse laterally. Methods A bioinformatic analysis of the P. multocida 17BRD-035 genome was conducted to determine if integrative conjugative elements (ICEs) carrying resistance genes, which hamper antibiotic treatment options locally, are in circulation in Australian feedlots. Results A novel element, ICE-PmuST394, was characterized in P. multocida 17BRD-035. It was also identified in three other isolates (two ST394s and a ST125) in Australia and is likely present in a genome representing P. multocida ST79 from the USA. ICE-PmuST394 houses a resistance module carrying two variants of the blaROB gene, blaROB-1 and blaROB-13, and the macrolide esterase gene, estT. The resistance gene combination on ICE-PmuST394 confers resistance to ampicillin and tilmicosin, but not to tulathromycin and tildipirosin. Our analysis suggests that ICE-PmuST394 is circulating both by clonal expansion and horizontal transfer but is currently restricted to a single feedlot in Australia. Conclusions ICE-PmuST394 carries a limited number of unusual antimicrobial resistance genes but has hotspots that facilitate genomic recombination. The element is therefore amenable to hosting more resistance genes, and therefore its presence (or dispersal) should be regularly monitored. The element has a unique molecular marker, which could be exploited for genomic surveillance purposes locally and globally.

show abstract

Exploring the intricacies of Pasteurella multocida dynamics in high-altitude livestock and its consequences for bovine health: A personal exploration of the yak paradox

Mo,

Nawaz,

Kulyar

et al. 2024

Microbial Pathogenesis

View full text Add to dashboard Cite

Genomic profiling of Pasteurella multocida isolated from feedlot cases of bovine respiratory disease

Cited by 5 publications

References 30 publications

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Identification and evolution of ICE-PmuST394: a novel integrative conjugative element in Pasteurella multocida ST394

Exploring the intricacies of Pasteurella multocida dynamics in high-altitude livestock and its consequences for bovine health: A personal exploration of the yak paradox

Contact Info

Product

Resources

About