Genomic profile of maize response to<i>Aspergillus flavus</i>infection

Kelley, Rowena Y.; Williams, W. Paul; Mylroie, John E.; Boykin, Debbie; Hawkins, Leigh K.; Windham, Gary L.; Brooks, Thomas D.; Bridges, Susan M.; Scheffler, Brian E.; Wilkinson, J. R.

doi:10.1080/15569540903089239

Cited by 16 publications

(15 citation statements)

References 39 publications

(42 reference statements)

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“…Similarly, using the maize Unigene 1-1.05 arrays, 236 genes were identified with significant differences in their expression levels in a study comparing resistant and susceptible inbred maize lines during 48 h of post-A. flavus infection, of which 135 genes were up-regulated in the susceptible, while 112 genes were upregulated in the resistant [75]. Multiple candidate genes involved in resistance to A. flavus infection were identified, several of which could be directly mapped to the maize chromosomes.…”

Section: Candidate Gene Identificationmentioning

confidence: 99%

Biotechnological advances for combating Aspergillus flavus and aflatoxin contamination in crops

et al. 2015

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a b s t r a c tAflatoxins are toxic, carcinogenic, mutagenic, teratogenic and immunosuppressive byproducts of Aspergillus spp. that contaminate a wide range of crops such as maize, peanut, and cotton. Aflatoxin not only affects crop production but renders the produce unfit for consumption and harmful to human and livestock health, with stringent threshold limits of acceptability. In many crops, breeding for resistance is not a reliable option because of the limited availability of genotypes with durable resistance to Aspergillus. Understanding the fungal/crop/environment interactions involved in aflatoxin contamination is therefore essential in designing measures for its prevention and control. For a sustainable solution to aflatoxin contamination, research must be focused on identifying and improving knowledge of host-plant resistance factors to aflatoxin accumulation. Current advances in genetic transformation, proteomics, RNAi technology, and marker-assisted selection offer great potential in minimizing preharvest aflatoxin contamination in cultivated crop species. Moreover, developing effective phenotyping strategies for transgenic as well as precision breeding of resistance genes into commercial varieties is critical. While appropriate storage practices can generally minimize post-harvest aflatoxin contamination in crops, the use of biotechnology to interrupt the probability of pre-harvest infection and contamination has the potential to provide sustainable solution.

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Section: Candidate Gene Identificationmentioning

confidence: 99%

Biotechnological advances for combating Aspergillus flavus and aflatoxin contamination in crops

et al. 2015

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“…These studies identified differentially expressed proteins from resistant versus susceptible maize genotypes both with [45][46][47][48] and without [49,50] infection of A. flavus during the experiment. Genome-wide association studies (GWAS) have also been brought to bear on the aflatoxin problem in maize.…”

Section: Other Aflatoxin Resistance Candidate Genesmentioning

confidence: 99%

Aflatoxin Resistance in Maize: What Have We Learned Lately?

Warburton

Williams

2014

Advances in Botany

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Aflatoxin contamination of maize grain is a huge economic and health problem, causing death and increased disease burden in much of the developing world and income loss in the developed world. Despite the gravity of the problem, deployable solutions are still being sought. In the past 15 years, much progress has been made in creating resistant maize inbred lines; mapping of genetic factors associated with resistance; and identifying possible resistance mechanisms. This review highlights this progress, most of which has occurred since the last time a review was published on this topic. Many of the needs highlighted in the last reviews have been addressed, and several solutions, taken together, can now greatly reduce the aflatoxin problem in maize grain. Continued research will soon lead to further solutions, which promise to further reduce and even eliminate the problem completely.

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“…The identification of genetic factors that may increase resistance to aflatoxin accumulation in maize has been accomplished by various methods. These include QTL mapping (reviewed in Mideros et al, 2013), protein studies (Chen et al, 2007; Pechanova et al, 2011), and gene expression analysis (Kelley et al, 2009). However, biparental mapping populations miss alleles that do not segregate between the parents of the population and many of the loci of smaller effect.…”

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confidence: 99%

Genome‐Wide Association Mapping of Aspergillus flavus and Aflatoxin Accumulation Resistance in Maize

Warburton¹,

Tang²,

Windham³

et al. 2015

Crop Science

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Contamination of maize (Zea mays L.) with aflatoxin, produced by the fungus Aspergillus flavus Link, has severe health and economic consequences. Efforts to reduce aflatoxin accumulation in maize have focused on identifying and selecting germplasm with natural host resistance factors, and several maize lines with significantly reduced aflatoxin accumulation have been identified. Past linkage mapping studies have identified quantitative trait loci (QTL) that consistently reduce aflatoxin levels in maize. In addition, an association mapping panel of 300 maize inbred lines was previously created specifically for the dissection of aflatoxin accumulation resistance. Here we report the results of a genome‐wide association study (GWAS) using this panel of testcrossed maize hybrids. Each of the inbred parents of the testcrossed hybrids was genotyped by sequencing to generate 261,184 robust single nucleotide polymorphisms (SNPs), and the entire panel was phenotyped for aflatoxin accumulation following inoculation with A. flavus in multilocation, replicated field trials. Results uncovered 107 SNPs associated with aflatoxin accumulation in one or more environments in the association panel at a probability level between 9.78 × 10−6 and 2.87 × 10−10. Eight SNP trait associations were found with a false discovery rate (FDR) of less than 10% (p < 3.83 × 10−7). These SNPs occur within the sequence of three uncharacterized genes. Variants in 25 other genomic regions showing high association values over more than one environment are also presented. These genomic regions are undergoing validation studies and will be of use to dissect the resistance to aflatoxin accumulation and improve this trait.

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Genomic profile of maize response toAspergillus flavusinfection

Cited by 16 publications

References 39 publications

Biotechnological advances for combating Aspergillus flavus and aflatoxin contamination in crops

Biotechnological advances for combating Aspergillus flavus and aflatoxin contamination in crops

Aflatoxin Resistance in Maize: What Have We Learned Lately?

Genome‐Wide Association Mapping of Aspergillus flavus and Aflatoxin Accumulation Resistance in Maize

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