Genomic portrait of ovarian follicle growth regulation in cattle

Zielak-Steciwko, Anna; Evans, A.C.O.

doi:10.1016/j.repbio.2016.07.003

Cited by 14 publications

(10 citation statements)

References 29 publications

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“…Of these, 755 genes were found to be upregulated, whereas 708 genes were downregulated. Well‐known FSH‐regulated genes among these DEGs include Fshr, Lhcgr, Cyp19a1 , natriuretic peptide precursor type C ( Nppc ), pregnancy‐associated plasma protein ( Pappa ), and low‐density lipoprotein receptor ( Ldlr; Gebremedhn et al, 2015; George et al, 2011; Hunzicker‐Dunn & Maizels, 2006; Irving‐Rodgers, Harland, Sullivan, & Rodgers, 2009; Kumar et al, 1997; Parakh et al, 2006; Richards, 1979, 2001; Zielak‐Steciwko & Evans, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…This difference in the number of DEGs could be attributed to differences in the time-line (48 h in our study vs. 4 h in the bovine study), source of granulosa cells for transcriptome analysis (in vivo vs. in vitro), hormone used to stimulate (eCG vs. recombinant human FSH) in addition to species differences (mouse vs. cow). In our study, the 755 upregulated genes included well-known FSH-induced genes including Fshr, Lhcgr, Cyp19a1, Nppc, Pappa, and Ldlr (Gebremedhn et al, 2015;George et al, 2011;Hunzicker-Dunn & Maizels, 2006;Irving-Rodgers et al, 2009;Kumar et al, 1997;Parakh et al, 2006;Richards, 1979Richards, , 2001Zielak-Steciwko & Evans, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Global analysis of FSH‐regulated gene expression and histone modification in mouse granulosa cells

Madogwe

Tanwar

Taibi

et al. 2020

Molecular Reproduction Devel

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Follicle-stimulating hormone (FSH) regulates ovarian follicular development through a specific gene expression program. We analyzed FSH-regulated transcriptome and histone modification in granulosa cells during follicular development. We used super-stimulated immature mice and collected granulosa cells before and 48 h after stimulation with equine chorionic gonadotropin (eCG). We profiled the transcriptome using RNA-sequencing (N = 3/time-point) and genome-wide trimethylation of lysine 4 of histone H3 (H3K4me3; an active transcription marker) using chromatin immunoprecipitation and sequencing (ChIP-Seq; N = 2/time-point). Across the mouse genome, 14,583 genes had an associated H3K4me3 peak and 63-66% of these peaks were observed within ≤1 kb promoter region. There were 72 genes with differential H3K4me3 modification at 48 h eCG (absolute log fold change > 1; false discovery rate [FDR] < 0.05) relative to 0 h eCG. Transcriptome data analysis showed 1463 differentially expressed genes at 48 h eCG (absolute log fold change > 1; FDR < 0.05). Among the 20 genes with differential expression and altered H3K4me3 modification, Lhcgr had higher H3K4me3 abundance and expression, while Nrip2 had lower H3K4me3 abundance and expression. Using ChIP-qPCR, we showed that FSH-regulated expression of Lhcgr, Cyp19a1, Nppc, and Nrip2 through regulation of H3K4me3 at their respective promoters. Transcript isoform analysis using Kallisto-Sleuth tool revealed 875 differentially expressed transcripts at 48 h eCG (b > 1; FDR < 0.05). Pathway analysis of RNA-seq data demonstrated that TGF-β signaling and steroidogenic pathways were regulated at 48 h eCG. Thus, FSH regulates gene expression in granulosa cells through multiple mechanisms namely altered H3K4me3 modification and inducing specific transcripts. These data form the basis for further studies investigating how these specific mechanisms regulate granulosa cell functions.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Global analysis of FSH‐regulated gene expression and histone modification in mouse granulosa cells

Madogwe

Tanwar

Taibi

et al. 2020

Molecular Reproduction Devel

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“…Post transcriptional regulation of LHCGR by miRNA in ovary has been reported in several species [21,56e58]. There are reports of miRNA expression and their specific roles in bovine ovary [59], including that of miR-222, which was reported by Hossain et al [25] as a possible regulator of LHCGR expression. Besides that, Salilew-Wondim et al [60] demonstrated the expression of miR-222 in theca and granulosa cells of bovine antral follicles with lower expression in granulosa cells from bovine dominant follicles.…”

Section: Discussionmentioning

confidence: 98%

Effect of superstimulation on the expression of microRNAs and genes involved in steroidogenesis and ovulation in Nelore cows

et al. 2018

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To better understand the impact of ovarian superstimulation on bovine follicular microenvironment, Nelore cows (Bos taurus indicus) were subjected to ovarian superstimulation with follicle stimulating hormone (FSH, n = 10; P-36 protocol) or FSH combined with eCG (n = 10; P-36/eCG protocol). Follicular fluid was analyzed for cholesterol concentration. Granulosa cells were analyzed by RT-qPCR to assess the expression of genes involved in steroidogenic and ovulatory and expression of microRNAs involved in final follicular development and luteinizing hormone/choriogonadotropin receptor (LHCGR) expression. Plasma concentration of estradiol was also measured. Follicular fluid from the P-36 group showed higher concentration of cholesterol than that of control (non-superstimulated) cows. Plasma concentration of estradiol was higher in the P-36/eCG group. Abundance of STAR and FSHR mRNAs were lower in granulosa cells from the P-36/eCG group. In contrast, LHCGR mRNA abundance was higher in superstimulated granulosa cells from the P-36 group and showed a pattern opposite to that of miR-222 expression. Ovarian superstimulation did not affect the expression of other markers (mmu-miR-202-5p, has-miR-873, has-miR-144, and their target genes, CREB, TGFBR2, and ATG7) of antral follicle development. However, the mRNA expression of VEGF pathway components was modulated by P-36 treatment. Taken together, these results demonstrate that superstimulatory protocols modify steroidogenic capacity, increase plasma estradiol, and regulate the abundance of VEGF system, LHCGR mRNA and suppress the expression of miR-222 in bovine granulosa cells.

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“…Thus, ovarian responses to hormonal stimulations were evaluated in follicles and cortex concerning the expression of receptors for both gonadotrophins ( FSHR and LHCGR ) and growth factors (constituted by proteins codified by TGFβR‐1 , BMPR1‐A and BMPR2 genes) in follicles. These genes were selected based on their importance for folliculogenesis process in pigs (Paradis et al., 2009) and cattle (Zielak‐Steciwko & Evans, 2016). In this study, primers were designed to amplify specifically target ( FSHR , LHCGR, TGFβR1 , BMPR1‐A and BMPR2 ) and housekeeping ( GAPDH , H2A and UXT ) genes.…”

Section: Discussionmentioning

confidence: 99%

Ovarian gene expression in collared peccary (Pecari tajacuLinnaeus, 1758) subjected to gonadotropin stimulation protocols

Viana

Freitas

Magalhães

et al. 2020

Reprod Domestic Animals

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Ovarian response of collared peccaries (Pecari tajacu), after hormonal stimulation with gonadotropin association (eCG/hCG), was accessed by both gene expression and follicular development. Thus, collared peccaries (n = 8) were treated with the dose used for sows (swine dose, SWD) or with dose adjusted for peccary's weight (allometric dose, ALD). The gene expression of receptors was evaluated for both gonadotropins (FSHR and LHCGR) and growth factors (proteins codified by TGFβR‐1, BMPR1‐A and BMPR2 genes) in antral follicles, cortex and corpora haemorrhagica (CH). Five days after gonadotropin injection, all females presented CH. The ovulation rate was similar (p > .05) between SWD (4.00 ± 1.17) and ALD (2.50 ± 0.43) group. The total number of follicles per animal and amounts of small (<3 mm), medium (3–5 mm) and large (>5 mm) follicles was similar among groups. However, SWD produced large follicles heavier than ALD group, as accessed by weight of follicular wall biopsies. Ovarian follicles expressed both gonadotropin and growth factor receptors at levels which are independent from gonadotropin dose. In conclusion, the two gonadotropin doses (SWD and ALD) can be used for ovarian stimulation of collared peccary. Additionally, FSH and growth factors (TGFβR‐1, BMPR1‐A and BMPR2) receptors are more expressed in the early follicle development, while LH receptor seems to be more important in the final of follicular growth.

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Genomic portrait of ovarian follicle growth regulation in cattle

Cited by 14 publications

References 29 publications

Global analysis of FSH‐regulated gene expression and histone modification in mouse granulosa cells

Global analysis of FSH‐regulated gene expression and histone modification in mouse granulosa cells

Effect of superstimulation on the expression of microRNAs and genes involved in steroidogenesis and ovulation in Nelore cows

Ovarian gene expression in collared peccary (Pecari tajacuLinnaeus, 1758) subjected to gonadotropin stimulation protocols

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