Genomic organization of the T-cell receptor γ gene and PCR detection of its clonal rearrangement in canine T-cell lymphoma/leukemia

Yagihara, Hiroko; Tamura, Kyoichi; Isotani, Mayu; Ono, Kenichiro; Washizu, Tsukimi; Bonkobara, Makoto

doi:10.1016/j.vetimm.2006.11.005

Cited by 38 publications

(71 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9,15 Briefly, genomic DNA was extracted from PBMCs using a commercial extraction kit. g For detection of clonal rearrangement of the TCRc gene, 2 different primers for V segments (Vca: 59-CGTGTACTACTGCG-CTGCCTGG-39 and Vcb: 59-GGCTGTATTACTGTG-CCTGCTGG-39) and 2 different primers for J segments (Jca: 59-TACCTTCTG(C/T)AAATATCTTGA-39 and Jcb: 59-TGTGCCAGGACCAAGCACTTTGTT-39) were used.…”

Section: Clonality Analysismentioning

confidence: 99%

“…Clonality analysis using polymerase chain reaction (PCR) amplification for dogs has been previously reported. 4 More recently, a similar assay was developed based on the canine genome database by targeting the T-cell receptor gamma chain (TCRc) gene 15 and the immunoglobulin heavy chain (IgH) gene. 9 Although the diagnostic value of PCR analysis has been demonstrated in several types of lymphoproliferative disorders, 7,[11][12][13][14] only limited studies have been done to identify neoplastic proliferation of lymphocytes in dogs with small-cell lymphocytosis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Yagihara

Uematsu²,

Koike³

et al. 2009

J VET Diagn Invest

Self Cite

View full text Add to dashboard Cite

Abstract. Lymphocytosis caused by neoplastic proliferation of small lymphocytes is occasionally difficult to distinguish by morphological examination from nonneoplastic lymphocytosis. To examine the clinical utility of gene rearrangement analysis for demonstrating neoplastic proliferation of small lymphocytes, gene rearrangement analysis was performed in comparison with immunophenotyping using peripheral lymphocytes in dogs with small lymphocytosis. Thirty-one dogs with small-cell lymphocytosis (8,100-884,300/ml) were enrolled. By immunophenotyping, lymphocytosis of all dogs was suggested to be neoplastic in nature based on the detection of marked expansion of phenotypically homogeneous lymphocytes or the presence of an aberrant antigen-expressing population of lymphocytes. In contrast, gene rearrangement analysis represented clonality in 27 dogs (detection rate of 87%). From the present study, gene rearrangement analysis was considered to be worthwhile to strengthen the evidence of neoplastic proliferation of small lymphocytes when coupled with immunophenotyping and to be a suitable diagnostic substitute if immunophenotyping is not available in clinical practice.

show abstract

Section: Clonality Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Yagihara

Uematsu²,

Koike³

et al. 2009

J VET Diagn Invest

Self Cite

View full text Add to dashboard Cite

show abstract

“…As a consequence, the immunohistochemical characterization of skin infiltrates is generally not useful to distinguish between neoplastic and nonneoplastic Tcell infiltrates, and the histologic detection of earlystage ETCL remains a big challenge in dermatopathology. 4,15 At present, only molecular biology approaches based on the analysis of T-cell receptor (TCR) genes can help to distinguish reactive T-cell infiltrates from T-cell lymphomas. 4,9,15 In dogs and other mammals, TCR is a complex gene that consists of a and b, or c and d chains.…”

Section: Introductionmentioning

confidence: 99%

“…4,15 At present, only molecular biology approaches based on the analysis of T-cell receptor (TCR) genes can help to distinguish reactive T-cell infiltrates from T-cell lymphomas. 4,9,15 In dogs and other mammals, TCR is a complex gene that consists of a and b, or c and d chains. The 4 chains are encoded by 4 distinct loci designated TCRa, TCRb, TCRc, and TCRd.…”

Section: Introductionmentioning

confidence: 99%

“…The 4 chains are encoded by 4 distinct loci designated TCRa, TCRb, TCRc, and TCRd. 4,15 The gene loci contain multiple segments coding for the variable (V), diversity (D), joining (J), and constant (C) regions of the TCRs. During T-cell development in the thymus, T lymphocytes somatically rearrange their antigen receptor genes, whereas all the other somatic cells keep the germ line (non-rearranged) forms of the genes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improved Polymerase Chain Reaction–Based Method to Detect Early-Stage Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides) in Formalin-Fixed, Paraffin-Embedded Skin Biopsy Specimens of the Dog

Chaubert

Sattler

et al. 2010

J VET Diagn Invest

View full text Add to dashboard Cite

Abstract. In the dog, early-stage epitheliotropic T-cell lymphoma (ETCL) can clinically and histologically mimic a large range of inflammatory dermatoses and often progresses rapidly to a more aggressive tumor stage. Early diagnosis of ETCL is essential to proceed with a specific oncologic therapy that is favorable for the prognosis. In the present study, an improved method for the detection of T-cell receptor gamma (TCRc) rearrangement was developed by designing a new set of consensus primers to amplify the different forms of rearranged canine TCRc gene sequences by polymerase chain reaction. The amplicons were analyzed by conventional polyacrylamide gel electrophoresis, which requires minimal specific equipment and may be performed in almost every pathology laboratory at low costs. The method proved to be highly specific and sensitive to detect early ETCL in formalin-fixed, paraffin-embedded biopsy specimens, providing an efficient tool for veterinary pathologists to distinguish early neoplastic from reactive cutaneous T-cell infiltrates (tumor-specific marker) or to discriminate T-cell lymphoma from B-cell lymphomas or nonlymphoid neoplasms (T-cell lineage marker). By direct sequencing analysis of amplified TCRc gene sequences, ETCL was found to rearrange exclusively the joining (J) 4 region, which suggests specific biology for primary cutaneous T-cell lymphomas. Also, a novel (seventh) functional J region in the TCRc gene, localized approximately 2.3 kb upstream of J5, was identified.

show abstract

Determination of Clonality

Goto‐Koshino¹,

Tsujimoto²

2022

Schalm's Veterinary Hematology

View full text Add to dashboard Cite

Genomic organization of the T-cell receptor γ gene and PCR detection of its clonal rearrangement in canine T-cell lymphoma/leukemia

Cited by 38 publications

References 13 publications

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Improved Polymerase Chain Reaction–Based Method to Detect Early-Stage Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides) in Formalin-Fixed, Paraffin-Embedded Skin Biopsy Specimens of the Dog

Determination of Clonality

Contact Info

Product

Resources

About