Genomic Organization of the Human SPOCK Gene and Its Chromosomal Localization to 5q31

Charbonnier, Frédéric; Jp, Périn; Mg, Mattei; Camuzat, Agnès; Bonnet, F; Gressin, Lætitia; Pm, Alliel

doi:10.1006/geno.1997.5199

Cited by 30 publications

(21 citation statements)

References 14 publications

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“…SPOCK (testican) was also a discrepancy early in our study, as it had been assigned to chromosome 21 (Cheng et al, 1994), which was inconsistent with our localization to 5q31. However, during the preparation of this article, its localization to 5q31 was confirmed by other investigators (Charbonnier et al, 1998).…”

Section: Figsupporting

confidence: 69%

A Radiation Hybrid Breakpoint Map of the Acute Myeloid Leukemia (AML) and Limb-Girdle Muscular Dystrophy 1A (LGMD1A) Regions of Chromosome 5q31 Localizing 122 Expressed Sequences

et al. 1999

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Section: Figsupporting

confidence: 69%

A Radiation Hybrid Breakpoint Map of the Acute Myeloid Leukemia (AML) and Limb-Girdle Muscular Dystrophy 1A (LGMD1A) Regions of Chromosome 5q31 Localizing 122 Expressed Sequences

et al. 1999

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“…The proteoglycan SPOCK1 (also known as testican 1) is involved in cell adhesion, invasion, and neurogenesis. SPOCK1 has several regions that are homologous to secreted protein acidic and rich in cysteine (SPARC)/Osteonectin and BM-40 (28). SPARC is an important mediator of tumor cell progression and has been implicated in a variety of diverse biological processes, including cell adhesion, proliferation, angiogenesis, tumor cell migration, and invasion (29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

Identification of Genes Differentially Expressed in Benign versus Malignant Thyroid Tumors

Prasad¹,

Somervell²,

Tufano³

et al. 2008

Clinical Cancer Research

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Purpose: Although fine-needle aspiration biopsy is the most useful diagnostic tool in evaluating a thyroid nodule, preoperative diagnosis of thyroid nodules is frequently imprecise, with up to 30% of fine-needle aspiration biopsy cytology samples reported as ''suspicious'' or ''indeterminate.'' Therefore, other adjuncts, such as molecular-based diagnostic approaches are needed in the preoperative distinction of these lesions. Experimental Design: In an attempt to identify diagnostic markers for the preoperative distinction of these lesions, we chose to study by microarray analysis the eight different thyroid tumor subtypes that can present a diagnostic challenge to the clinician. Results: Our microarray-based analysis of 94 thyroid tumors identified 75 genes that are differentially expressed between benign and malignant tumor subtypes. Of these, 33 were overexpressed and 42 were underexpressed in malignant compared with benign thyroid tumors. Statistical analysis of these genes, using nearest-neighbor classification, showed a 73% sensitivity and 82% specificity in predicting malignancy. Real-time reverse transcription^PCR validation for 12 of these genes was confirmatory. Western blot and immunohistochemical analyses of one of the genes, high mobility group AT-hook 2, further validated the microarray and real-time reverse transcription^PCR data. Conclusions: Our results suggest that these 12 genes could be useful in the development of a panel of markers to differentiate benign from malignant tumors and thus serve as an important first step in solving the clinical problem associated with suspicious thyroid lesions.

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“…In addition, SPOCK1, another gene specifically involved in brain cell adhesion and invasion (Bonnet et al, 1992;Charbonnier et al, 1998), was highly expressed in PA. The purified gene product has been shown to inhibit cell attachment and neurite extension in culture and to inhibit membrane type 1 and 2 matrix metalloproteinases (Edgell et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Identification of genes differentially expressed in glioblastoma versus pilocytic astrocytoma using Suppression Subtractive Hybridization

et al. 2005

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Glioblastoma (GBM) is a highly malignant glioma, which has the propensity to infiltrate throughout the brain in contrast to pilocytic astrocytoma (PA) of the posterior fossa, which does not spread and can be cured by surgery. We have used Suppression Subtractive Hybridization to define markers that better delineate the molecular basis of brain invasion and distinguish these tumor groups. We have identified 106 genes expressed in PA versus GBM and 80 genes expressed in GBM versus PA. Subsequent analysis identified a subset of 20 transcripts showing a common differential expression pattern for the two groups. GBM differs from PA by the expression of five genes involved in invasion and angiogenesis: fibronectin, osteopontin, chitinase-3-like-1 (YKL-40), keratoepithelin and fibromodulin. PA differs from GBM by the expression of genes related to metabolism (apolipoprotein D), proteolysis (protease-serine-11), receptor and signal transduction (PLEKHB1 for Pleckstrin-Homology-domain-containingprotein-family-B-member-1), transcription/translation (eukaryotic-translation-elongation-factor-1-a1) processes and cell adhesion (SPOCK1 for SPARC/Osteonectin-CWCV-kazal-like-domains-proteoglycan). The expression of these genes was confirmed by real-time quantitative RT-PCR and immunohistochemistry. This study highlights the crucial role of brain invasion in GBM and identifies specific molecules involved in this process. In addition, it offers a restricted list of markers that accurately distinguish PA from GBM.

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Genomic Organization of the Human SPOCK Gene and Its Chromosomal Localization to 5q31

Cited by 30 publications

References 14 publications

A Radiation Hybrid Breakpoint Map of the Acute Myeloid Leukemia (AML) and Limb-Girdle Muscular Dystrophy 1A (LGMD1A) Regions of Chromosome 5q31 Localizing 122 Expressed Sequences

A Radiation Hybrid Breakpoint Map of the Acute Myeloid Leukemia (AML) and Limb-Girdle Muscular Dystrophy 1A (LGMD1A) Regions of Chromosome 5q31 Localizing 122 Expressed Sequences

Identification of Genes Differentially Expressed in Benign versus Malignant Thyroid Tumors

Identification of genes differentially expressed in glioblastoma versus pilocytic astrocytoma using Suppression Subtractive Hybridization

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