Genomic Organization of Plum Pox Virus and Recombinant Dna Technology to Combat Plum Pox Virus.

Ravelonandro, M.; Teycheney, Pierre‐Yves; Tavert, G.; Wetzel, Thierry; Monsion, Marie; Delbos, R.; Dunez, Jean

doi:10.17660/actahortic.1990.283.34

Cited by 2 publications

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“…2. These recombinant plasmids include nt 48 to 2996 of the virus genome, and were obtained from the fusion of two recombinant plasmids, pS 337 and pS 50 [25]. The plasmid pP zX HC contains a deletion of 192 nucleotides (from nt 1375 to 1566) consisting of two small Eco RV fragments of 50 and 142 bp-relative to pPHC (Fig.…”

Section: Recombinant Plasmids and In Vitro Transcriptionmentioning

confidence: 99%

Immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants

Ravelonandro¹,

Peyruchaud²,

Garrigue³

et al. 1993

Archives of Virology

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Tobacco plants (Nicotiana tabacum cv. Xanthi) have been transformed via Agrobacterium tumefaciens vectors, with cDNAs corresponding to the plum pox virus (PPV) cistron 2 encoding helper component (HC-Pro) and with the first two and half cistrons of the PPV genome. Presence of the HC-Pro in PPV-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the PPV HC-Pro. The results suggest that two proteases are involved in the processing of the PPV N-terminal polyprotein to yield a protein of 48 k (HC-Pro). HC-Pro autolytically cleaves at its carboxyl-terminus and a proteolytic activity, probably associated with the protein (P1) encoded by the cistron 1, is required for the cleavage in planta between the proteins derived from cistrons 1 and 2.

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Section: Recombinant Plasmids and In Vitro Transcriptionmentioning

confidence: 99%