Genomic organization of an intron-containing sperm protein 17 gene (Sp17-1) and an intronless pseudogene (Sp17-2) in humans: a new model

Buchli, Rico; Jong, Ann De; Robbins, Dick L.

doi:10.1016/s0167-4781(02)00478-5

Cited by 8 publications

(5 citation statements)

References 57 publications

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“…[14][15][16] Recently, an intronless human Sp17 psuedogene (Sp17-2) has been identified on chromosome 10, attributed to RNA retroposition. 16 The human Sp17 protein has 151 amino acids and can be divided into 3 regions. The N-terminal portion exhibits sequence similarity to the human cAMP-dependent protein kinase type II regulatory subunit (RII), whereas the carboxy end of the molecule contains an ''IQ'' motif typically found in calmodulin binding proteins.…”

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confidence: 99%

Clinical significance of sperm protein 17 expression and immunogenicity in esophageal cancer

Gupta

Sharma

et al. 2007

Intl Journal of Cancer

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We recently identified sperm protein 17 (Sp17) transcripts in esophageal squamous cell carcinomas (ESCCs) by differential display. This study was designed to determine the clinical significance of Sp17 protein in different stages of esophageal tumorigenesis and to test the hypothesis that aberrant localization of Sp17 protein to immunosurveillant site may lead to production of antiSp17 antibodies in serum, which may be of clinical relevance in ESCCs. Sp17 transcripts were detected by RT-PCR in 26 of 30 (86%) ESCCs, while no transcripts were detected in normal esophageal tissues. Rabbit polyclonal antibody was raised against an immunogenic peptide of Sp17 and used to evaluate protein expression by immunohistochemistry. Expression of Sp17 protein was observed in 60/80 (75%) of ESCCs and 27/30 (90%) dysplastic tissues, while no detectable Sp17 expression was observed in 13 distant histologically normal epithelia. Sixteen of the 60 immunopositive ESCCs showed nuclear expression in addition to cytoplasmic localization of the protein. The circulating levels of anti-Sp17 antibodies, determined by ELISA, were significantly elevated in ESCC patients when compared with normal subjects (p < 0.001). Increasing Sp17 antibody titers were observed to be associated with the progressive disease in 4 patients. In conclusion, the study demonstrates expression of Sp17 protein in esophageal tumor as well as dysplastic tissues, suggesting it to be an early event in the development of ESCC. To our knowledge, this is the first report showing elevated levels of anti-Sp17 antibodies in ESCC patients. ' 2007 Wiley-Liss, Inc.Key words: esophageal squamous cell carcinoma; ELISA; immunohistochemistry; RT-PCR; Sp17Esophageal cancer (EC) ranks as the 6th most common cancer among males and 9th most common cancer among females, globally.1 Squamous cell carcinoma is the predominant histological subtype of esophageal cancer, characterized by high mortality rate and strong association with dietary habits and lifestyle in India.

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confidence: 99%

Clinical significance of sperm protein 17 expression and immunogenicity in esophageal cancer

Gupta

Sharma

et al. 2007

Intl Journal of Cancer

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“…The gene encoding Sp17 is mapped by sequence database to chromosome 11 in human. It contains five exons and four introns (5). There are two transcriptional start sites, designated Sp17‐1a and Sp17‐1b.…”

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Combined real time PCR and immunohistochemical evaluation of sperm protein 17 as a cancer‐testis antigen

Zhang

Wang

Robinson

et al. 2004

European J of Haematology

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We previously identified sperm protein 17 (Sp17) as a normal testicular protein aberrantly expressed in a proportion of multiple myeloma (MM). However, recent studies have generated controversies on the normal tissue expression of Sp17 and whether or not it is a suitable target for immunotherapy. In this study, we have used a combination of real time polymerase chain reaction and immunohistochemistry on a large panel of normal tissues. Although Sp17 transcripts could be detected in some normal tissues, the levels of expression were <2% of those in normal testis. In contrast, Sp17+ myeloma cells expressed 3-18% of normal testis levels of Sp17 transcript. Immunohistochemistry using two Sp17 murine monoclonal antibodies, each directed at a non-overlapping B-cell epitope, showed Sp17 protein to be expressed only in testis and not any other normal tissues. Specificity of binding of the antibodies to testis was also confirmed in competitive binding assays. Our results therefore further suggest Sp17 as a cancer-testis antigen in MM and support its suitability as a target for immunotherapy.

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“…Buchli et al (2002) already described, in humans, the genomic organization of an introncontaining sperm protein 17 gene (Sp17-1) and an intronless gene with ORF interrupted by stop codons, giving rise to a pseudogene (Sp17-2). As far as we know, this is the first time that genomic variants of the same gene ORF are reported in plants.…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization and physical mapping of two fucosyltransferase genes inMedicago truncatula

Castilho¹,

Cunha²,

Afonso³

et al. 2005

Genome

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Fucosyltransferases catalyse fucose transfer onto oligosaccharides. Two fucosylated structures have been identified in plants: the alpha1,4-fucosylated Lewis-a epitope and the alpha1,3-fucosylated core. Here we report the cloning, genomic characterization, and physical mapping of two genes encoding proteins similar to alpha1,4-fucosyltransferase (EC 2.4.1.65, MtFUT1) and alpha1,3-fucosyltransferase (EC 2.4.1.214, MtFUT2) in Medicago truncatula. Analysis of the genomic organization of the fucosyltransferase genes in M. truncatula, revealed the presence of two genomic variants of the MtFUT1 gene coding sequence, one containing a single intron and the other intronless, whereas in MtFUT2, the gene coding region is interrupted by four introns. Using for the first time fluorescence in situ hybridization (FISH) to physically map fucosyltransferase genes in plants, this study reveals a high genomic dispersion of these genes in Medicago. The MtFUT1 genes are mapped on chromosomes 4, 7, and 8, colocalizing on three of the five MtFUT2 loci. Chromosomes 1 and 5 carry the additional MtFUT2 loci. Moreover, the intensity of the FISH signals reveals marked differences in the number of gene copies per locus for both genes. Simultaneous mapping of rRNA genes on chromosome 5 shows that several MTFUT2 gene loci are inserted within the rDNA array. Insertions of coding DNA sequences into the rDNA repeats were never reported to date.

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Genomic organization of an intron-containing sperm protein 17 gene (Sp17-1) and an intronless pseudogene (Sp17-2) in humans: a new model

Cited by 8 publications

References 57 publications

Clinical significance of sperm protein 17 expression and immunogenicity in esophageal cancer

Clinical significance of sperm protein 17 expression and immunogenicity in esophageal cancer

Combined real time PCR and immunohistochemical evaluation of sperm protein 17 as a cancer‐testis antigen

Genomic characterization and physical mapping of two fucosyltransferase genes inMedicago truncatula

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