Genomic organization and promoter analysis of the mouse ADP-ribosylarginine hydrolase gene

Enhanced Sensitivity to Cholera Toxin in ADP-Ribosylarginine Hydrolase-Deficient Mice

“…Only one hydrolase has been identified in mammalian tissues, consistent with the ex-istence of only one ADP-ribose-arginine hydrolase gene (2). Its function, presumably, is to reverse the action of the ADPribosyltransferase and thereby regulate cellular levels of proteins containing ADP-ribose-arginine (30,43).…”

“…ADPRH Ϫ/Ϫ cells expressing ADPRH wild-type cDNA are designated ADPRH Ϫ/Ϫ * and ADPRH Ϫ/Ϫ cells expressing the ADPRH mutant protein are designated ADPRH Ϫ/Ϫ **. After selection, these reconstituted (rescued) ADPRH cells were grown in DMEM supplemented with 10% NBCS, penicillin (10,000 units/ml), streptomycin (10 mg/ml), and zeocin (1 mg/ml) (Invitrogen) at 37°C in a humidified atmosphere with 5% CO 2 .…”

The ARH and Macrodomain Families of α-ADP-ribose-acceptor Hydrolases Catalyze α-NAD+ Hydrolysis

“…25 ARH1 is the ubiquitously expressed product of a single gene. 26 Arh1-deficient and Arh1 +/− mice spontaneously developed tumors and Arh1 +/− mouse embryonic fibroblasts (MEFs) developed tumors in nude mice. 27 Further, tumors formed in nude mice by Arh1 +/− MEFs contained mutations in the remaining Arh1 gene, consistent with the hypothesis that Arh1 is a tumor suppressor gene.…”

“…The ARH gene family consists of three 39-kD proteins (ARH1–3) that share similarities in primary sequence . ARH1 is the ubiquitously expressed product of a single gene .…”