Genomic organization and mRNA expression of manganese superoxide dismutase (Mn-SOD) from Hemibarbus mylodon (Teleostei, Cypriniformes)

Cho, Young Sun; Lee, Sang Yoon; Bang, In Chul; Kim, Dong Soo; Nam, Yoon Kwon

doi:10.1016/j.fsi.2009.07.003

Cited by 60 publications

(25 citation statements)

References 36 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were also observed in Mn-SODs of Litopenaeus vannamei, Haliotis discus discus and H. mylodon (Ekanayake et al 2006;Gómez-Anduro et al, 2006;Cho et al, 2009). Therefore, these findings suggest that…”

Section: Discussionsupporting

confidence: 82%

“…There was a report that in Hemibarbus mylodon, Mn-SOD expression in the liver and kidney were remarkably up-regulated by LPS injection, Edwardsiella tarda challenge, and heavy metal exposure (Cho et al, 2009). Moreover, Wang and colleagues (2010) recently found that the gene expression of Mn-SOD was significantly increased after immunostimulation in red swamp crayfish.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Wang and colleagues (2010) recently found that the gene expression of Mn-SOD was significantly increased after immunostimulation in red swamp crayfish. Several reports also have evaluated differential SOD modulation in fish organs during exposure to xenobiotic compounds (Shi et al, 2005;Vega-López et al, 2007;An et al, 2008;Cho et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)

Wang

Osatomi

Nagatomo

et al. 2011

Comparative Biochemistry and Physiology Part B: Biochemistry an

View full text Add to dashboard Cite

Manganese-superoxide dismutase (Mn-SOD) from Japanese flounder (Paralichthys olivaceus) hepatopancreas has been purified with high purification (781-fold) and recovery (10.8%). The molecular mass of the purified enzyme was estimated to be 26 kDa by SDS-PAGE under reducing conditions. In activity staining by native-PAGE, the Japanese flounder Mn-SOD gave three active bands and exhibited KCN-insensitive activity. In addition, the electrophoretic mobility of this enzyme was observed to be faster than that of Japanese flounder Cu,Zn-SOD. On the other hand, the N-terminal amino acid sequence of this Mn-SOD was determined to be 16 amino acid residues, and the sequence showed high homology to other Mn-SODs but not Japanese flounder Cu,Zn-SOD. Analysis of nucleotide and deduced amino acid sequences revealed that the Mn-SOD cDNA consisted of a 64 bp 5'-non-coding region, a 675 bp open reading frame encoding 225 amino acids, and a 465 bp 3'-non-coding region. The first 27 amino acids containing a mitochondria-targeting signal were highly conserved among other Mn-SODs.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)

Wang

Osatomi

Nagatomo

et al. 2011

Comparative Biochemistry and Physiology Part B: Biochemistry an

View full text Add to dashboard Cite

show abstract

“…Additionally, Reyes-Becerril et al (2008) reported that dietary supplementation of live yeast resulted in the elevation of SOD activity in the gilthead seabream (Sparus aurata) liver, which may support, at least in part, our finding of the stimulated transcription of hmsod1. Further, results of this study are similar to our recent observations of the upregulated expression of the mitochondrial type of manganese SOD (Mn-SOD; sod2) from this species upon bacterial challenge (Cho et al, 2009c). Increased sod expression upon bacterial challenge has been generally recognized as an antioxidant defense action to scavenge or neutralize the ROS released during the proinflammatory cytokine response to bacterial toxins (Marikovsky et al, 2003).…”

Section: Stimulated Expression Of Hmsod1 In Response To Bacterial Chasupporting

confidence: 89%

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Lee¹,

Kim²,

Bang³

et al. 2011

Fisheries and aquatic sciences

View full text Add to dashboard Cite

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

show abstract

“…In addition, the CREBP motif was also exclusively predicted in the Wap65-2 isoform. Considering that NF-кB is a key player in innate immune defense systems, especially the Toll signaling pathway (Hayden and Ghosh, 2004;Cho et al, 2009), the exclusive prediction of NF-кB in the Wap65-2 promoter suggests that Wap65-2 isoform to be more closely related to inflammation-mediated modulation than the Wap65-1 isoform. The presence of cytokine responsive and/or stressrelated motifs has also been reported in Wap65 genes in a few species, although comparative examinations of the two isoforms within a single fish species are limited to a few fish species (Hirayama et al, 2003;Nakaniwa et al, 2005;Sha et al, 2008).…”

Section: Nucleic Acid Preparation and Qpcr Analysismentioning

confidence: 99%

Genomic Organization and Isoform-Dependent Expression Patterns of Wap65 genes in Various Tissues during Immune Challenges in the Mud Loach Misgurnus mizolepis

Kim¹,

Cho²,

Lee³

et al. 2014

Fisheries and aquatic sciences

Self Cite

View full text Add to dashboard Cite

Genomic organization, including the structural characteristics of 5´-flanking regions of two 65-kDa protein (WAP65) isoform genes associated with warm temperature acclimation, were characterized and their transcriptional responses to immune challenges were examined in the intestine, kidney and spleen of the mud loach (Misgurnus mizolepis; Cypriniformes). Both mud loach Wap65 isoform genes displayed a 10-exon structure that is common to most teleostean Wap65 genes. The two mud loach Wap65 isoforms were predicted to possess various stress-and immune-related transcription factor binding sites in their regulatory regions; however, the predicted motif profiles differed between the two isoforms, and the inflammation-related transcription factor binding motifs, such as NF-κB and CREBP sites, were more highlighted in the Wap65-2 isoform than the Wap65-1 isoform. The results of qRT-PCR indicated that experimental immune challenges using Edwardsiella tarda, lipopolysaccharide or polyI:C induced the Wap65-2 isoform more than Wap65-1 isoform, although modulation patterns in response to these challenges were tissue-and stimulant-dependent. This study confirms that functional diversification between the two mud loach Wap65 isoforms (i.e., closer involvement of Wap65-2 in the acute phase of inflammation and innate immunity) occurs at the mRNA level in multiple tissues, and suggests that such differential modulation patterns between the two isoforms are related to the different transcription factor binding profiles in their regulatory regions.

show abstract

Genomic organization and mRNA expression of manganese superoxide dismutase (Mn-SOD) from Hemibarbus mylodon (Teleostei, Cypriniformes)

Cited by 60 publications

References 36 publications

Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)

Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Genomic Organization and Isoform-Dependent Expression Patterns of Wap65 genes in Various Tissues during Immune Challenges in the Mud Loach Misgurnus mizolepis

Contact Info

Product

Resources

About