Genomic organization and expression of simian foamy virus type 3 (SFV-3)

Renne, Rolf; Friedl, Erika M.; Schweizer, Matthias; Fleps, Ute; Turek, Robert; Neumann‐Haefelin, Dieter

doi:10.1016/0042-6822(92)90026-l

Cited by 81 publications

(55 citation statements)

References 43 publications

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“…There are limited nucleotide sequence data on primate foamy virus genomes available, which support our view (Fltigel et al, 1987(Fltigel et al, , 1990Maurer et al, 1988;Mergia et al, 1990;Kupiec et al, 1991;Renne et al, 1992;Herchenr6der et al, 1994). Pol and Env proteins were found to be highly conserved among all foamy virus isolates.…”

Section: Discussionsupporting

confidence: 53%

“…In both clones the first ATG codon was from the b e l l ORF. In a similar way to the HFV bet cDNAs clone, phage lambda clone 20A starts in the vicinity of the SFV-1 internal promoter (Mergia, 1994) and shows an identical splicing pattern to the HFV bet clone pA65, using splice sites conserved in all foamy virus genomes sequenced so far (Flfigel et al, 1987;Kupiec et al, 1991 ;Renne et al, 1992;Herchenr6der et al, 1994). The SFV-1 bet sequence starts with the ORF-1 ATG and is spliced into the ORF-2 frame after 93 codons of ORF-1.…”

Section: Characterization Of H F V and Sfv-1 Bet And Gag Proteinsmentioning

confidence: 99%

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Reactivity of primate sera to foamy virus Gag and Bet proteins

Hahn¹,

Baunach²,

Bräutigam³

et al. 1994

Journal of General Virology

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In order to establish criteria for the serodiagnosis of foamy virus infections we investigated the extent to which sera from infected individuals of human and primate origin react with structural and non-structural virus proteins in immunoblot assays. Using lysates from infected cells as the source of virus antigen, antibodies were preferentially detected against the Gag proteins and the non-structural Bet protein. Both the Gag precursor molecules of 70 and 74K apparent M r and the cytoplasmic 60K M r Bet protein were found to be phosphorylated, the latter being synthesized in large amounts in infected cells. Rabbit antiserum raised against recombinant human foamy virus (HFV) Gag major capsid protein cross-reacted with foamy viruses of chimpanzee, gorilla, orang-utan, rhesus monkey and African green monkey origin. This was reflected by a broad cross-reactivity of the respective monkey sera to the Gag proteins of the various foamy virus isolates. Cross-reactivity of antisera against the Bet protein was restricted to viruses from man and the great apes. Recombinant Gag and Bet proteins expressed in prokaryotes or in insect cells were readily recognized by foamy virus-positive primate sera. Screening serum samples from chimpanzees with HFV Gag and Bet proteins expressed by recombinant baculoviruses revealed that 18 out of 35 (52 %) were positive for Gag antibodies. Of these, 13 (72%) showed antibodies against the Bet protein, indicating that Bet antigen is of value in serological screening for foamy virus infections.

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Section: Discussionsupporting

confidence: 53%

Section: Characterization Of H F V and Sfv-1 Bet And Gag Proteinsmentioning

confidence: 99%

Reactivity of primate sera to foamy virus Gag and Bet proteins

Hahn¹,

Baunach²,

Bräutigam³

et al. 1994

Journal of General Virology

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“…Thus, TAFsFv-1 does not stimulate the HFV L TR {Mergia et al., 1992). Enhancement of SFV-3 L TR activity by TAFsFV-I has been described (Renne et al, 1993) which might reflect the cfoser relatedness of these two viruses (Renne et al, 1992). However, T AF sFv.…”

Section: Jntroductionmentioning

confidence: 99%

“…BEL-1 (or TAFHFV• for transactivator of foamy virus} acts on a U3 DNA elernent of the viral LTR (Rethwilm et al, 1991;Keller er al., 1991;Venkatesh et al, 1991 Renne et al, 1992).…”

Section: Jntroductionmentioning

confidence: 99%

“…3 responsiveness to a heterofogous promoter, irrespective of their orientation, have been mapped (Renne et al:, 1993). HFV and SFV U3 regions are highly divergent compared to Rand U5 sequences (Renne et al, 1992). This is reflected by a low degree of HFV and SFV T AF amino acid homology compared to the env and pol proteins (Mergia et al .. 1991; Renne er a/., 1992).…”

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confidence: 99%

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BEL-1 Transactivator Responsive Sequences in the Long Terminal Repeat of Human Foamy Virus

Erlwein

Rethwilm

1993

Virology

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Cis-regulatory elements in the long terminal repeat (LTR) of human foamy virus (HFV) were identified by using L TR mutants to transiently express the chforamphenicol acetyf-transferase gene after co-transfection with an expression plasmid for the virus bel-1 (transactivator) gene. The R~U5 region and an element in the 5' U3 region were found to negatively influence HFV geneexpress Ion. The complete BEL-1 responsive regionwas mapped to axtend from nucleotide position -471 to position -93 relative to the start of transcription. Within this region, three e,ements were ldentified that in the homologaus or a heterologous (SV40) activator of viral transcription (Cullen, 1991 ). The viral bel-1 gene has been identified to encode for a 36 k nuclear phosphoprotein that augments HFV lang terminal repeat (LTR} directed transcription in a variety of mammalian and in avian cells (Rethwilm et al., 1991;Keller et al., 1991;Venkatesh et al., 1991, Venkatesh et al., 1993 1 To whom correspondence and reprint requests shoufd be addressed. 0042-6822193 $5.00Copyright@ 1993 by Academic Press,lnc.All rights o# reproduction in any torm reserved. 256Jn previous studies on the mapping of BEL-1 responsive elements in HFV U3, the BEL-1 target regionwas located upstream of positions -94 (Keller et al., 1991) and -62 (Venkatesh et al., 1991) respectivery. relative to the start of transcription. A more definitive analysis has been performed on the TAF response region of SFV-1 and SFV-3. Two regions in the SFV~ 1 L TR between positions -1196 and -880 and between -403 and -125 were identified to respond to TAFsFv-1 ; the latter element was also found to act as an inducible enhancer in an orientation independent manner in the context of a heterologous promoter (Mergia et al., 1992). ln the SFV-3 LTR, two regions (-637/-496 and -496/-180, respectively) that confer TAFsFv. 3 responsiveness to a heterofogous promoter, irrespective of their orientation, have been mapped (Renne et al:, 1993). HFV and SFV U3 regions are highly divergent compared to Rand U5 sequences (Renne et al., 1992). This is reflected by a low degree of HFV and SFV T AF amino acid homology compared to the env and pol proteins (Mergia et al .. 1991; Renne er a/., 1992). Thus, TAFsFv-1 does not stimulate the HFV L TR {Mergia et al., 1992). Enhancement of SFV-3 L TR activity by TAFsFV-I has been described (Renne et al., 1993) which might reflect the cfoser relatedness of these two viruses (Renne et al., 1992). However, T AF sFv. 3 does not stimulate reporter geneexpressiondriven by the SFV-1 LTR (Renne et al., .1993).While the results of these experiments suggest a highly specific mechanism of foamy virus transactiva-

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Spumaviruses

2009

Simian Virology

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Genomic organization and expression of simian foamy virus type 3 (SFV-3)

Cited by 81 publications

References 43 publications

Reactivity of primate sera to foamy virus Gag and Bet proteins

Reactivity of primate sera to foamy virus Gag and Bet proteins

BEL-1 Transactivator Responsive Sequences in the Long Terminal Repeat of Human Foamy Virus

Spumaviruses

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