Genomic Organization and Chromosomal Mapping of the Bovine Fas/APO-1 Gene

Yoo, Jakyoung; Stone, R. T.; Kappes, S. M.; Toldo, S. Solinas; Fries, R.; Beattie, Craig W.

doi:10.1089/dna.1996.15.377

Cited by 10 publications

(8 citation statements)

References 46 publications

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“…Beattie (unpubl.) Kemp and Teale (1991 ) Yoo et al (1996) (1992) Markers not listed in Tables 1 and 3 have been referenced in one of the following: ; Bishop et al (1994); Moore et al (1994); Stone et al (1995Stone et al ( , 1997. aGenBank accession number.…”

Section: Discussionmentioning

confidence: 99%

A second-generation linkage map of the bovine genome.

Kappes¹,

Keele²,

Stone³

et al. 1997

Genome Res.

467

333

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We report a bovine linkage map constructed with 1236 polymorphic DNA markers and 14 erythrocyte antigens and serum proteins. The 2990-cM map consists of a sex-specific, X chromosome linkage group and 29 sex-averaged, autosomal linkage groups with an average interval size of 2.5 cM. The map contains 627 new markers and 623 previously linked markers, providing a basis for integrating the four published bovine maps. Orientation and chromosomal assignment of all the linkage groups, except BTA20 and BTA22, was provided by 88 markers that were assigned previously to chromosomes. This map provides sufficient marker density for genomic scans of populations segregating quantitative trait loci (QTL) and subsequent implementation of marker-assisted selection (MAS) mating schemes.

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Section: Discussionmentioning

confidence: 99%

A second-generation linkage map of the bovine genome.

Kappes¹,

Keele²,

Stone³

et al. 1997

Genome Res.

467

333

View full text Add to dashboard Cite

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“…As with growth‐related genes, the promoters of many pro‐apoptotic and anti‐apoptotic genes have been found to contain ‘Sp1 sites’. These genes including those for Fas and Fas ligand (Yoo et al, 1996; McClure et al, 1999), Bcl‐2, Bcl‐3, Bcl‐x, Bak, and Bax (Grillot et al, 1997; Ulrich et al, 1997; Dong et al, 1999; Igata et al, 1999; Rebollo et al, 2000), survivin (Li and Altieri, 1999), TGF‐β and its receptors (Kim et al, 1989; Malipiero et al, 1990; Humphries et al, 1994; Ammanamanchi and Brattain, 2001; Wolf et al, 2001), and TRAIL and TNF α (Leitman et al, 1992; Wang et al, 2000b). Thus, it is not surprising that increasing evidence indicates that Sp/KLF family members are involved in the regulation of apoptosis.…”

Section: Alterations In Sp/klf Family Members Related To Cancermentioning

confidence: 99%

Sp1 and krüppel‐like factor family of transcription factors in cell growth regulation and cancer

Black

Azizkhan‐Clifford

2001

Journal Cellular Physiology

953

870

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The Sp/KLF family contains at least twenty identi®ed members which include Sp1-4 and numerous kru È ppel-like factors. Members of the family bind with varying af®nities to sequences designated as`Sp1 sites' (e.g., GC-boxes, CACCC-boxes, and basic transcription elements). Family members have different transcriptional properties and can modulate each other's activity by a variety of mechanisms. Since cells can express multiple family members, Sp/KLF factors are likely to make up a transcriptional network through which gene expression can be ®ne-tuned. Sp1 site'-dependent transcription can be growth-regulated, and the activity, expression, and/or post-translational modi®cation of multiple family members is altered with cell growth. Furthermore, Sp/KLF factors are involved in many growth-related signal transduction pathways and their overexpression can have positive or negative effects on proliferation. In addition to growth control, Sp/KLF factors have been implicated in apoptosis and angiogenesis; thus, the family is involved in several aspects of tumorigenesis. Consistent with a role in cancer, Sp/ KLF factors interact with oncogenes and tumor suppressors, they can be oncogenic themselves, and altered expression of family members has been detected in tumors. Effects of changes in Sp/KLF factors are context-dependent and can appear contradictory. Since these factors act within a network, this diversity of effects may arise from differences in the expression pro®le of family members in various cells. Thus, it is likely that the properties of the overall network of Sp/KLF factors play a determining role in regulation of cell growth and tumor progression.

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“…This product was ligated into pGEM-T Easy (Promega) and the recombinant plasmid transformed into JM109 competent cells (Promega). The 517 bp insert was DNA sequenced and shown to be 97% homologous to the published sequence for bovine Fas (Yoo et al 1996) (GenBank accession no. NM_174662).…”

Section: Northern and Slot Blottingmentioning

confidence: 99%

Effects of glucocorticoids on Fas gene expression in bovine blood neutrophils

Chang

Madsen

Toelboell

et al. 2004

Journal of Endocrinology

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Blood neutrophils are extremely short-lived cells that are programmed for rapid apoptosis after differentiation in bone marrow. Recently, glucocorticoids have been shown to prolong survival of human and rodent neutrophils, but the mechanisms and implications for leukocyte homeostasis and health are unclear. In this study, we investigated the effects of endogenous and exogenous glucocorticoids on Fas expression in bovine neutrophils because Fas is a major death receptor that stimulates apoptosis in circulating cells. Our study subjects were four periparturient dairy cows whose blood concentrations of cortisol peaked at calving, 15 dexamethasone-treated steers and three untreated steers whose neutrophils were exposed to dexamethasone in vitro. Fas mRNA abundance changes in collected neutrophils were monitored numerous times relative to the in vivo glucocorticoid challenges, and the relationships between these data and circulating neutrophil counts were estimated by correlation analyses. Fas mRNA and protein abundance, caspase 8 activity, and survival of neutrophils in vitro were also monitored in the presence and absence of dexamethasone. In the periparturient cows, Fas mRNA abundance in circulating neutrophils showed a sharp decrease between calving and 12 h postpartum. Based on PROC CORR analysis (SAS), this correlated negatively with blood neutrophil count (r=-0.634; P=0.0009) and serum cortisol concentration (r=-0.659; P<0.0001), but showed no relationship with serum progesterone or estradiol concentrations (P > or =0.09). Administration of dexamethasone to steers also caused a pronounced reduction in neutrophil Fas mRNA abundance that persisted for 12 h and correlated negatively with blood neutrophil count (r=-0.748; P=0.0021). In vitro, dexamethasone caused dose-dependent loss of GR proteins from the cytosol of neutrophils concurrently with Fas mRNA downregulation, which was inhibited by the glucocorticoid receptor (GR) antagonist, RU486. Dexamethasone treatment of cultured neutrophils also reduced surface Fas expression, spontaneous and sFasL-induced caspase 8 activity, and rate of apoptosis in the cells. Taken together, these in vivo and in vitro results suggest that glucocorticoids inhibit Fas expression in bovine blood neutrophils via GR activation, possibly contributing to the cells' increased longevity in culture and the pronounced neutrophilia observed in parturient cows and hormone-treated steers. We thus conclude that glucocorticoid-activated GR may change the homeostasis of circulating neutrophils, in part through its negative effects on Fas gene expression and downstream apoptosis signaling pathways.

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Genomic Organization and Chromosomal Mapping of the Bovine Fas/APO-1 Gene

Cited by 10 publications

References 46 publications

A second-generation linkage map of the bovine genome.

A second-generation linkage map of the bovine genome.

Sp1 and krüppel‐like factor family of transcription factors in cell growth regulation and cancer

Effects of glucocorticoids on Fas gene expression in bovine blood neutrophils

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