Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production

Abstract

Moderate halophilic bacteria thrive in saline conditions and produce biosurfactant (BS) which facilitates the oil scavenging activity in the oil polluted surroundings. Production of such unusual bioactive molecules plays a vital role for their survival in an extreme and adverse environment. Current research deals with isolation of Planococcus maritimus strain SAMP MCC 3013 from Indian Arabian coastline sea water for BS production. The bacterium tolerated up to 2.7 M NaCl demonstrating osmotic stress bearable physiological systems. We used integrated approach to explore the genomic insight of the strain SAMP and displayed the presence of gene for BS biosynthesis. The genome analysis revealed this potential to be intrinsic to the strain. Preliminary screening techniques viz., surface tension (SFT), drop collapse (DC) and oil displacement (OD) showed SAMP MCC 3013 as a potent BS producer. BS reduced SFT of phosphate buffer saline (PBS) pH: 7.0 from 72 to 30 mN/m with a critical micelle concentration (CMC) value of 1.3 mg/mL. Subsequent investigation on chemical characterization, using thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance ( 1 H NMR and 13 C NMR) and liquid chromatography mass spectrometry (LC-MS) revealed terpene containing BS having sugar, lipid moieties. The genomic sequence analysis of P. maritimus SAMP showed complete genes in the pathway for the synthesis of terpenoid. Probably terpenoid is the accountable backbone molecule for the BS production, but the later stages of terpenoid conversion to the BS could not be found. Moreover, it is important to highlight that till today; no single report documents the in-detailed physico-chemical characterization of BS from Planococcus sp. Based on genomic and functional properties, the term terpene containing BS is denoted for the surfactant produced by P. maritimus .

Microbiome-based environmental monitoring of a dairy processing facility highlights the challenges associated with low microbial-load samples

“…and Kocuria sp . have previously been reported in food processing environments (Vishnivetskaya and Kathariou, 2005; Røder et al ., 2015), Planococcus sp ., although not well characterised with few genomes available, are regarded as halotolerant, water-associated microorganisms, rather than food processing contaminants (Waghmode et al ., 2019). The generation of this MAG and further generation of MAGs, will accelerate the identification of food chain microbes through sequencing-based approaches in the future.…”

Pseudomonas aeruginosa RTE4: A Tea Rhizobacterium With Potential for Plant Growth Promotion and Biosurfactant Production

“…The complete production of BS was carried out at 30 • C, 150 rpm for 7 days which was observed at 12 h intervals. Simultaneously biomass (g/L) during BS fermentation was determined by measuring dried cell weight (Waghmode et al, 2019). Additionally, different parameters like drop size (mm), SFT (mN/m), and optical density (at 600 nm) were checked at 12 h interval.…”

“…Stability of the RTE4 BS was assessed at different pH and temperatures. CMC solution of the BS was prepared in different buffer solutions with pH ranging from 2 to 12 and incubated for 24 h followed by SFT measurement to check the effect of pH (Waghmode et al, 2019) on SFT reducing abilities of BS. CMC solution of BS was incubated for 24 h at different temperatures viz.…”

Planococcus Species – An Imminent Resource to Explore Biosurfactant and Bioactive Metabolites for Industrial Applications

“…Under such nutrient-deficient and challenging circumstances, marine microbes withstand and compete through the production of a unique magical molecules ( Gudiña et al, 2015 ). Therefore, the marine ecosystem grips an immense assure to invent an innovative bioactive compounds like enzymes, vitamins, antibiotics, drugs, biosurfactant (BS), and bioemulsifier (BE) ( Satpute et al, 2010 ; Gudiña et al, 2016 ; Waghmode et al, 2019 ). Marine microbes produce not only diverse types of BSs but also other bioactive molecules under extreme surroundings of salinity, fluctuations in temperatures and pH, availability of limited nutrients, and increased UV exposure ( de Carvalho, 2018 ; Naughton et al, 2019 ).…”

“…Possibly this was the first report detailing the physico-chemical properties of BS derived form Planococcus sp. ( Waghmode et al, 2019 ).…”