Genomic insights into Brucella

Rajendhran, Jeyaprakash

doi:10.1016/j.meegid.2020.104635

Cited by 28 publications

(26 citation statements)

References 105 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Virulence factor genes are involved in synthesis of lipopolysaccharide (LPS), intracellular survival, iron uptake, secretion system, and motility/flagellar genes for Brucella ( Rajendhran, 2021 ). A total of 43 virulence factor genes were predicted in all strains except for strain 2007BM-1 ( ricA and wbkA are absent).…”

Section: Resultsmentioning

confidence: 99%

“…The core genome alignment-based phylogeny or SNP-based phylogeny deduced from genome sequence data and comparative genomics are applicable for discrimination of strains. Of these, phylogeny based on pangenome SNPs will cover all regions of genome including intergenic regions and, hence, covers more genome regions compared with MLST or core genome phylogeny ( Rajendhran, 2021 ). Molecular surveillance of pathogens is essential to identify the antigenic changes so as to develop a better vaccine candidate.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome Sequencing and Comparative Genomics of Indian Isolates of Brucella melitensis

et al. 2021

View full text Add to dashboard Cite

Brucella melitensis causes small ruminant brucellosis and a zoonotic pathogen prevalent worldwide. Whole genome phylogeny of all available B. melitensis genomes (n = 355) revealed that all Indian isolates (n = 16) clustered in the East Mediterranean lineage except the ADMAS-GI strain. Pangenome analysis indicated the presence of limited accessory genomes with few clades showing specific gene presence/absence pattern. A total of 43 virulence genes were predicted in all the Indian strains of B. melitensis except 2007BM-1 (ricA and wbkA are absent). Multilocus sequence typing (MLST) analysis indicated all except one Indian strain (ADMAS-GI) falling into sequence type (ST 8). In comparison with MLST, core genome phylogeny indicated two major clusters (>70% bootstrap support values) among Indian strains. Clusters with <70% bootstrap support values represent strains with diverse evolutionary origins present among animal and human hosts. Genetic relatedness among animal (sheep and goats) and human strains with 100% bootstrap values shows its zoonotic transfer potentiality. SNP-based analysis indicated similar clustering to that of core genome phylogeny. Among the Indian strains, the highest number of unique SNPs (112 SNPs) were shared by a node that involved three strains from Tamil Nadu. The node SNPs involved several peptidase genes like U32, M16 inactive domain protein, clp protease family protein, and M23 family protein and mostly represented non-synonymous (NS) substitutions. Vaccination has been followed in several parts of the world to prevent small ruminant brucellosis but not in India. Comparison of Indian strains with vaccine strains showed that M5 is genetically closer to most of the Indian strains than Rev.1 strain. The presence of most of the virulence genes among all Indian strains and conserved core genome compositions suggest the use of any circulating strain/genotypes for the development of a vaccine candidate for small ruminant brucellosis in India.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome Sequencing and Comparative Genomics of Indian Isolates of Brucella melitensis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Brucellosis is a widespread zoonosis caused by pathogenic Brucella , a group of Gram-negative facultative intracellular bacteria ( 6 , 7 ). Although the main hosts of Brucella are sheep, cattle, swine, and goat, it can be transmitted to humans when the contaminated milk, dairy products, and meat are ingested, or when individuals are in contact with infected animals ( 8 ).…”

Section: Discussionmentioning

confidence: 99%

“…Although the main hosts of Brucella are sheep, cattle, swine, and goat, it can be transmitted to humans when the contaminated milk, dairy products, and meat are ingested, or when individuals are in contact with infected animals ( 8 ). In the recent years, 12 species of Brucella are isolated both from marine and land mammals ( 2 , 7 , 9 ), four of them have the zoonotic characteristic, the most virulent species that can cause human brucellosis is B. melitensis , followed successively by B. abortus, B. suis , and B. canis ( 1 ). In this case, the patient was infected by B. melitensis after contacting with infected sheep and suffered from CNS infection with multiple symptoms.…”

Section: Discussionmentioning

confidence: 99%

Case Report: Metagenomic Next-Generation Sequencing Confirmed a Case of Central Nervous System Infection With Brucella melitensis in Non-endemic Areas

Cao

Cai

et al. 2021

Front. Med.

View full text Add to dashboard Cite

Brucellosis is a highly contagious zoonotic disease caused by bacteria that belong to the genus Brucella. It is a major endemic disease in northern China. We reported a rare case of central nervous system (CNS) infection caused by Brucella melitensis in a patient living in non-endemic areas. The medical history of the patient included chronic headache and trunk numbness. Based on the presented clinical symptoms and medical examinations, a clinical diagnosis of binocular uveo-encephalitis was made in the local hospital. The patient's symptoms were unrelieved after being treated with empiric therapy. Soon after, the patient was admitted to our hospital because of the obnubilation and coma in the trip. We ran a few examinations and sent the cerebrospinal fluid (CSF) for metagenomic next-generation sequencing (mNGS) immediately. The Magnetic resonance imaging (MRI) examination was unremarkable, and bilateral mastoid inflammation was attached. Metagenomic next-generation sequencing suggested a CNS infection caused by Brucella melitensis. Then, the results of the serum agglutination test and quantitative polymerase chain reaction assay also confirmed that. After being treated with doxycycline, rifampin, and cefatriaxone, consciousness of the patient was restored and headache diminished. Two months later, a lumbar puncture was used to check the pressure of the CSF, and the total course of treatment was more than 6 months. This case highlighted the potential value of mNGS in early clinal diagnosis. We believe that mNGS may be a complementary method for rapid identification of infection of CNS caused by the pathogen.

show abstract

“…There are as many as 12 species but only 4 are pathologically virulent for causing human infection. These are, Brucellamelitensis (from sheep; highest pathogenicity), Brucellasuis (from pigs; high pathogenicity), Brucellaabortus (from cattle; moderate pathogenicity), Brucellacanis (from dogs; moderate pathogenicity) [3][4] .For diagnosis careful history taking is very important as almost every case of brucella has a positive contact history which could be direct or indirect.…”

Section: Introductionmentioning

confidence: 99%

A Middle Aged Man Presented with Prolonged Fever and Low Back Pain Diagnosed as Brucellosis

2021

View full text Add to dashboard Cite

Brucellosis is a Zoonotic disease which is not so common health problem in Bangladesh. For its variable presentation it creates diagnostic challenge for the clinicians. Although Brucellosis is a treatable and curable condition sometimes delay in diagnosis and lack of insight for the disease creates high morbidity. In this case report we have presented a case of brucellosis presented with prolong fever, joint pain with complications which created dilemma in diagnosis in a tertiary care private hospital of Dhaka. The purpose of presenting this case report is to create concern among the clinicians regarding the variablepresentation of Brucellosis& emphasizing to clinical judgment over investigations, especially in limited resources. Bangladesh J Medicine July 2022; 33(1) : 89-93

show abstract

Genomic insights into Brucella

Cited by 28 publications

References 105 publications

Genome Sequencing and Comparative Genomics of Indian Isolates of Brucella melitensis

Genome Sequencing and Comparative Genomics of Indian Isolates of Brucella melitensis

Case Report: Metagenomic Next-Generation Sequencing Confirmed a Case of Central Nervous System Infection With Brucella melitensis in Non-endemic Areas

A Middle Aged Man Presented with Prolonged Fever and Low Back Pain Diagnosed as Brucellosis

Contact Info

Product

Resources

About