Genomic Insights into Bacterial DMSP Transformations

Moran, Mary Ann; Reisch, Chris R.; Kiene, Ronald P.; Whitman, William B.

doi:10.1146/annurev-marine-120710-100827

Cited by 159 publications

(172 citation statements)

References 79 publications

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“…Wide variation in the percent of dissolved DMSP converted to DMS in ocean surface waters (3% to 30%; has most frequently been attributed to the energetic benefit to bacteria of acquiring pre-reduced sulfur rather than expending three ATP equivalents to reduce seawater sulfate Pinhassi et al, 2005;Moran et al, 2012). This 'bacterial sulfur demand' hypothesis proposes that demethylation is favored by marine bacteria when reduced sulfur is limiting because the sulfur from DMSP can be assimilated into biomass through the demethylation pathway but not the cleavage pathway (Todd et al, 2009).…”

Section: Does the Sulfur Demand Hypothesis Fit?mentioning

confidence: 99%

“…In contrast, DMSPdegrading members of the Roseobacter and SAR116 lineages can, like HTCC2255, carry genes for both demethylation and cleavage (Newton et al, 2010;Moran et al, 2012), and therefore more directly influence the fate of DMSP. Emerging technological capabilities for conducting taxon-specific observations of gene regulation in the ocean are allowing us to explore, for the first time, the diversity of gene regulation strategies among the key bacterial groups affecting partitioning of organic sulfur between the ocean and atmosphere.…”

Section: Dms + Acrylatementioning

confidence: 99%

“…Factors proposed to influence this regulatory juncture include bacterial sulfur demand Simó , 2001), ultraviolet light stress (Slezak et al, 2007;Levine et al, 2012) and osmolyte requirements Reisch et al, 2008), but thus far none has satisfactorily explained the large variations in DMSP transformation patterns observed over time and space in the ocean. Although DMS emission to the atmosphere accounts for only a small fraction of the DMSP synthesized annually by marine phytoplankton (o2% of the 42000 Tg DMSP-S y À 1 ; Moran et al, 2012), it contributes B40% of the atmospheric S burden (Simó, 2001) and impacts atmospheric chemistry both directly and through its degradation products (Kirkby et al, 2011;Quinn and Bates, 2011;Chen and Jang, 2012). Future changes in the environmental conditions that regulate these pathways (Six et al, 2013) have the potential to alter the radiation status of Earth.…”

Section: Introductionmentioning

confidence: 99%

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Single-taxon field measurements of bacterial gene regulation controlling DMSP fate

et al. 2015

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The 'bacterial switch' is a proposed regulatory point in the global sulfur cycle that routes dimethylsulfoniopropionate (DMSP) to two fundamentally different fates in seawater through genes encoding either the cleavage or demethylation pathway, and affects the flux of volatile sulfur from ocean surface waters to the atmosphere. Yet which ecological or physiological factors might control the bacterial switch remains a topic of considerable debate. Here we report the first field observations of dynamic changes in expression of DMSP pathway genes by a single marine bacterial species in its natural environment. Detection of taxon-specific gene expression in Roseobacter species HTCC2255 during a month-long deployment of an autonomous ocean sensor in Monterey Bay, CA captured in situ regulation of the first gene in each DMSP pathway (dddP and dmdA) that corresponded with shifts in the taxonomy of the phytoplankton community. Expression of the demethylation pathway was relatively greater during a high-DMSP-producing dinoflagellate bloom, and expression of the cleavage pathway was greater in the presence of a mixed diatom and dinoflagellate community. These field data fit the prevailing hypothesis for bacterial DMSP gene regulation based on bacterial sulfur demand, but also suggest a modification involving oxidative stress response, evidenced as upregulation of catalase via katG, when DMSP is demethylated.

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Section: Does the Sulfur Demand Hypothesis Fit?mentioning

confidence: 99%

Section: Dms + Acrylatementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single-taxon field measurements of bacterial gene regulation controlling DMSP fate

et al. 2015

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“…DMSP is transformed to DMS, a volatile compound influencing global temperature but with a cooling effect, through its effects on cloud cover (Andreae, 1990). Functional genomics applied to natural communities recently reveals two different DMSP degrading pathways mediated by different genes and, therefore, microorganisms (Moran et al, 2012); one of them is the demethylation of DMSP facilitating retention of carbon and sulfur in the marine microbial food web and another is by cleavage of DMSP to DMS with important consequences for the ocean-atmosphere sulfur flux.…”

Section: Introductionmentioning

confidence: 99%

Methane production induced by dimethylsulfide in surface water of an upwelling ecosystem

Florez-Leiva¹,

Damm

Farı́as

2013

Progress in Oceanography

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a b s t r a c tCoastal upwelling ecosystems are areas of high productivity and strong outgassing, where most gases, such as N 2 O and CH 4 , are produced in subsurface waters by anaerobic metabolisms. We describe seasonal CH 4 variation as well as potential mechanisms producing CH 4 in surface waters of the central Chile upwelling ecosystem (36°S). Surface waters were always supersaturated in CH 4 (from 125% up to 550%), showing a clear seasonal signal triggered by wind driven upwelling processes (austral springsummer period), that matched with the periods of high chlorophyll-a and dimethylsulfoniopropionate (DMSP) levels. Methane cycling experiments, with/without the addition of dimethylsulfide (including 13 C-DMS) and acetylene (a nonspecific inhibitor of CH 4 oxidation) along with monthly measurements of CH 4 , DMSP and other oceanographic variables revealed that DMS can be a CH 4 precursor. Net CH 4 cycling rates (control) fluctuated between À0.64 and 1.44 nmol L À1 d À1 . After the addition of acetylene, CH 4 cycling rates almost duplicated relative to the control, suggesting a strong methanotrophic activity. With a spike of DMS, the net CH 4 cycling rate significantly increased relative to the acetylene and control treatment. Additionally, the d 13 C values of CH 4 at the end of the incubations (after addition of 13 C enriched-DMS) were changed, reaching À32‰ PDB compared to natural values between À44‰ and À46‰ PDB. These findings indicate that, in spite of the strong CH 4 consumption by methanotrophs, this upwelling area is an important source of CH 4 to the atmosphere. The effluxes are derived partially from in situ surface production and seem to be related to DMSP/DMS metabolism.

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“…Thingstad et al, 1998;Pinhassi et al, 2006). In particular, P scarcity was particularly limiting to some Roseobacter clades (Pinhassi et al, 2006), which are abundant in the North Sea area (Zubkov et al, 2001(Zubkov et al, , 2002 and important DMS producers (Gonzalez et al, 1999;Moran et al, 2012). Results showed that if the proportion of bacteria able to transform DMSP into DMS decreased, the magnitude of DMS emissions decreased as well, and the maximal DMS emission simulated in 2002 decreased from 5.5 to 0.95 mmol S m −2 y −1 (Fig.…”

Section: Discussionmentioning

confidence: 99%

How phosphorus limitation can control climate-active gas sources and sinks

Gypens

Borges

Ghyoot

2017

Journal of Marine Systems

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Since the 1950's, anthropogenic activities have increased nutrient river loads to European coastal areas. Subsequent implementation of nutrient reduction policies have led to considerably reduction of phosphorus (P) loads from the mid-1980's, while nitrogen (N) loads were maintained, inducing a P limitation of phytoplankton growth in many eutrophied coastal areas such as the Southern Bight of the North Sea (SBNS). When dissolved inorganic phosphorus (DIP) is limiting, most phytoplankton organisms are able to indirectly acquire P from dissolved organic P (DOP). We investigate the impact of DOP use on phytoplankton production and atmospheric fluxes of CO 2 and dimethylsulfide (DMS) in the SBNS from 1951 to 2007 using an extended version of the R-MIRO-BIOGAS model. This model includes a description of the ability of phytoplankton organisms to use DOP as a source of P. Results show that primary production can increase up to 30% due to DOP uptake under limiting DIP conditions. Consequently, simulated DMS emissions also increase proportionally while CO 2 emissions to the atmosphere decrease, relative to the reference simulation without DOP uptake.

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Genomic Insights into Bacterial DMSP Transformations

Cited by 159 publications

References 79 publications

Single-taxon field measurements of bacterial gene regulation controlling DMSP fate

Single-taxon field measurements of bacterial gene regulation controlling DMSP fate

Methane production induced by dimethylsulfide in surface water of an upwelling ecosystem

How phosphorus limitation can control climate-active gas sources and sinks

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