Genomic insights into a new acidophilic, copper-resistantDesulfosporosinusisolate from the oxidized tailings area of an abandoned gold mine

Abstract

Microbial sulfate reduction in acid mine drainage is still considered to be confined to anoxic conditions, although several reports have shown that sulfate-reducing bacteria occur under microaerophilic or aerobic conditions. We have measured sulfate reduction rates of up to 60 nmol S cm(-3) day(-1) in oxidized layers of gold mine tailings in Kuzbass (SW Siberia). A novel, acidophilic, copper-tolerant Desulfosporosinus sp. I2 was isolated from the same sample and its genome was sequenced. The genomic analysis and physiological data indicate the involvement of transporters and additional mechanisms to tolerate metals, such as sequestration by polyphosphates. Desulfosporinus sp. I2 encodes systems for a metabolically versatile life style. The genome possessed a complete Embden-Meyerhof pathway for glycolysis and gluconeogenesis. Complete oxidation of organic substrates could be enabled by the complete TCA cycle. Genomic analysis found all major components of the electron transfer chain necessary for energy generation via oxidative phosphorylation. Autotrophic CO2 fixation could be performed through the Wood-Ljungdahl pathway. Multiple oxygen detoxification systems were identified in the genome. Taking into account the metabolic activity and genomic analysis, the traits of the novel isolate broaden our understanding of active sulfate reduction and associated metabolism beyond strictly anaerobic niches.

Effects of Air on Microbial Community and Tailing Wastewater Remediation in Reducing Bacteria Remediation Process

“…In Air+ group, the obvious increase of Desulfosporosinus biomass implied Desulfosporosinus is not the strict anaerobes [18]. Several papers have shown that some sulfate-reducing bacteria occur under microaerophilic or aerobic conditions, and reported the use and resistance mechanism of Desulfosporosinus to oxygen [18,19]. The results of Desulfosporosinus were consistent with the previous reports about sulfate-reducing bacteria.…”

“…The increase of biomass is more obvious in Air+ group, especially the anaerobic sulfate-reducing bacteria Desulfosporosinus. In Air+ group, the obvious increase of Desulfosporosinus biomass implied Desulfosporosinus is not the strict anaerobes [18]. Several papers have shown that some sulfate-reducing bacteria occur under microaerophilic or aerobic conditions, and reported the use and resistance mechanism of Desulfosporosinus to oxygen [18,19].…”

Temporal Responses of Microbial Communities to Anaerobic Soil Disinfestation

“…In addition to Azotobacter, we identified Oxobacter, Desulfotomaculum, and Desulfosporosinus abundances as being significantly correlated with nifH abundances. These genera also have the genomic potential for nitrogen fixation [49][50][51]. Our results here and in the field trial indicate that nitrogen fixation may be an Finally, on day 9, we compared soil microbiomes derived from DNA and RNA (cDNA) extractions to determine if there was a difference in the present versus active components of the communities (Fig.…”

Genome sequence of the copper resistant and acid-tolerant Desulfosporosinus sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area

“…Desulfosporosinus acidiphilus [2] and Desulfosporosinus acididurans [19], both isolated from mining environments, represent the only two validly described, moderately acidophilic sulfate-reducing bacteria. Two other acidophilic Desulfosporosinus have been isolated and their genomes are available [1], [15]. Desulfosporosinus spp.…”

“…Desulfosporosinus spp. are also known for their tolerance to metals, in particular to copper [1], [14], [15]. Recently, a novel acidophilic, copper-resistant Desulfosporosinus sp.…”