Genomic in situ hybridization differentiates between A/D- and C-genome chromatin and detects intergenomic translocations in polyploid oat species (genus <i>Avena</i>)

Jellen, Eric N.; Gill, B. S.; Cox, T. S.

doi:10.1139/g94-087

Cited by 147 publications

(137 citation statements)

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“…A number of authors have attempted to identify individual C-genome chromosomes in hexaploid oats by GISH and FISH (e.g. Chen and Armstrong, 1994;Jellen et al, 1994;Leggett and Markhand, 1995;Yang et al, 1999). Fominaya et al (1995) and Linares et al (1996) performed FISH analysis using a repeated DNA sequence (pAm1) specific to C-genome chromosomes.…”

Section: Discussionmentioning

confidence: 99%

“…At the moment, the GISH was unable to separate the A-and D-genome chromosomes in hexaploid oats. But absolute discrimination between A/D-and C-genome chromosomes has been demonstrated by several oat workers (Jellen et al, 1994;Chen and Armstrong, 1994;Leggett and Markhand, 1995;Yang et al, 1999). These authors observed small C-genome segments translocated onto A-or D-genome chromosomes (abbreviated as C-A/D translocation) and vice versa (abbreviated as A/D-C) for A-or D-segments translocated onto C-genome chromosomes.…”

Section: Introductionmentioning

confidence: 99%

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Intergenomic translocations of polyploid oats (genus Avena) revealed by genomic in situ hybridization.

2000

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Wild and cultivated hexaploid oats share the same genomes (AACCDD) and display a considerable level of interspecific variation in both plant and chromosome morphology. The GISH was utilized to detect the interspecific genomic compositions in four hexaploid and two tetraploid oats using total genomic DNA of Avena eriantha (a C-genome diploid) as probe. Intergenomic translocations between A/D and C-genome chromosomes were frequently observed in hexaploid and tetraploid species. In the hexaploid, two pairs of A/D genome segments on C-genome chromosome (A/D-C) translocation and four to six pairs of C-genome segments on A/D genome chromosome (C-A/D) translocation were clearly identified whilst the number of A/D-C translocations was constant among species. In the tetraploid A. maroccana (AACC), a pair of A-C and four pairs of C-A translocations were observed. Moreover, the A/D translocation segments on chromosome 5C was detected only in A. byzantina and A. maroccana, whilst A/D-C translocations were observed on the 1C and 7C of A. sativa, A. fatua and A. sterilis. A. byzantina did however also carry the 1C rearrangement. This result shows that A. byzantina has retained a similar genomic constitution to the tetraploid ancestor of hexaploid oats, A. maroccana. Three pairs of A-C translocations were detected only in A. murphyi (AACC), and two pairs of those were the 1C and 7C as well as the three hexaploid species except A. byzantina.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intergenomic translocations of polyploid oats (genus Avena) revealed by genomic in situ hybridization.

2000

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“…The D genome of the hexaploids is considered to have substantial similarities with the A genome based on GISH results (Chen and Armstrong, 1994;Jellen et al, 1994;Hayasaki et al, 2000). On the other hand, discrimination of the closely related A and D genomes is possible using an A genomespecific AS120a sequence (Linares et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Genomic and polyploid evolution in genus Avena as revealed by RFLPs of repeated DNA sequences

Morikawa

Nishihara

2009

Genes Genet. Syst.

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Phylogenetic relationships and genome affinities were investigated by utilizing all the biological Avena species consisting of 11 diploid species (15 accessions), 8 tetraploid species (9 accessions) and 4 hexaploid species (5 accessions). Genomic DNA regions of As120a, avenin, and globulin were amplified by PCR. A total of 130 polymorphic fragments were detected out of 156 fragments generated by digesting the PCR-amplified fragments with 11 restriction enzymes. The number of fragments generated by PCR-amplification followed by digestion with restriction enzymes was almost the same as those among the three repeated DNA sequences. A high level of genetic distance was detected between A. damascena (Ad) and A. canariensis (Ac) genomes, which reflected their different morphology and reproductive isolation. The A. longiglumis (Al) and A. prostrata (Ap) genomes were closely related to the As genome group. The AB genome species formed a cluster with the AsAs genome artificial autotetraploid and the As genome diploids indicating near-autotetraploid origin. The A. macrostachya is an outbreeding autotetraploid closely related with the C genome diploid and the AC genome tetraploid species. The differences of genetic distances estimated from the repeated DNA sequence divergence among the Avena species were consistent with genome divergences and it was possible to compare the genetic intra-and inter-ploidy relationships produced by RFLPs. These results suggested that the PCR-mediated analysis of repeated DNA polymorphism can be used as a tool to examine genomic relationships of polyploidy species.

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“…Through the use of chromosome-specific fluorescent in situ hybridi-zation (FISH) markers, at least nine reciprocal translocations have been detected in tetraploid Nicotiana tabacum, confirming that most tobacco chromosomes are associated with one of the two parental genomes [88]. Based on whole-genome in situ hybridization analysis, five reciprocal translocations have been found within the tetraploid Avena sativa genome, with as many as 18 detected in hexaploid Avena sativa [89]. Using FISH and other methods, we have confirmed the genetic parentage of 4nAT and verified the heterologous hybrid nature of these allotetraploid fish [1].…”

Section: Phenotypic and Genotypic Variation Of 4nat Lineagesmentioning

confidence: 92%

Research advances in animal distant hybridization

Zhang

Chen

et al. 2014

Sci. China Life Sci.

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Distant hybridization refers to crosses between two different species or higher-ranking taxa that enables interspecific genome transfer and leads to changes in phenotypes and genotypes of the resulting progeny. If progeny derived from distant hybridization are bisexual and fertile, they can form a hybrid lineage through self-mating, with major implications for evolutionary biology, genetics, and breeding. Here, we review and summarize the published literature, and present our results on fish distant hybridization. Relevant problems involving distant hybridization between orders, families, subfamilies, genera, and species of animals are introduced and discussed, with an additional focus on fish distant hybrid lineages, genetic variation, patterns, and applications. Our review serves as a useful reference for evolutionary biology research and animal genetic breeding.

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Genomic in situ hybridization differentiates between A/D- and C-genome chromatin and detects intergenomic translocations in polyploid oat species (genus Avena)

Cited by 147 publications

References 0 publications

Intergenomic translocations of polyploid oats (genus Avena) revealed by genomic in situ hybridization.

Intergenomic translocations of polyploid oats (genus Avena) revealed by genomic in situ hybridization.

Genomic and polyploid evolution in genus Avena as revealed by RFLPs of repeated DNA sequences

Research advances in animal distant hybridization

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