Genomic imprinting is disrupted in interspecific Peromyscus hybrids

Vrana, Paul B.; Guan, Xiao; Ingram, Robert S.; Tilghman, Shirley M.

doi:10.1038/3833

Cited by 170 publications

(155 citation statements)

References 27 publications

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“…Also, Igf 2 is an imprinted gene, and it has been shown recently that imprinting may be disrupted in interspecific Peromyscus hybrids (Vrana et al, 1998). When we compared Igf 2 expression in X Msp and X Mmu placentas, two differences were seen.…”

Section: Discussionmentioning

confidence: 90%

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Proliferation and growth factor expression in abnormally enlarged placentas of mouse interspecific hybrids

Zechner

Hemberger

Constância

et al. 2002

Developmental Dynamics

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It has been shown previously that abnormal placental growth occurs in crosses and backcrosses between different mouse (Mus) species. In such crosses, late gestation placentas may weigh between 13 and 848 mg compared with a mean placental weight of approximately 100 mg in late gestation M. musculus intraspecific crosses. A locus on the X-chromosome was shown to segregate with placental dysplasia. Thus in the (M. musculus ؋ M. spretus)F1 ؋ M. musculus backcross, placental hyperplasia cosegregates with a M. spretus derived X-chromosome. Here we have investigated whether increased cell proliferation and aberrant expression of two genes that are involved in placental growth control, Igf2 and Esx1, may cause, or contribute to placental hyperplasia. Increased bromodeoxyuridine labeling of nuclei, reflecting enhanced proliferation, was indeed observed in hyperplastic placentas when compared with normal littermate placentas. Also, increased expression of Igf 2 was seen in giant cells and spongiotrophoblast. However, when M. musculus ؋ M. spretus F1 females were backcrossed with males that were heterozygous for a targeted mutation of the Igf 2 gene, placentas that carried a M. spretus derived X-chromosome and were negative for a functional Igf 2 allele exhibited an intermediate placental phenotype. Furthermore, in early developmental stages of placental hyperplasia, we observed a decreased expression of the X-chromosomal Esx1 gene. This finding suggests that abnormal expression of both Igf 2 and Esx1 contributes to abnormal placental development in mouse interspecific hybrids. However, Esx1 is not regulated by IGF2.

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Section: Discussionmentioning

confidence: 90%

“…However, recently it was suggested that in the placental hybrid dysgenesis effects described in Peromyscus, autosomal imprinted genes may play a major role (Vrana et al, 1998(Vrana et al, , 2000. An autosomal imprinted gene which has been shown to be pivotal in control of placental growth is Igf 2 (Baker et al, 1993;Lopez et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Proliferation and growth factor expression in abnormally enlarged placentas of mouse interspecific hybrids

Zechner

Hemberger

Constância

et al. 2002

Developmental Dynamics

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“…Defects in the trans-acting machinery that regulates imprinting, such as DNA methyltransferase deficiency (Lei et al, 1996;Bourc'his et al, 2001;Hata et al, 2002;Kaneda et al, 2004;Arima et al, 2006) or mutations causing familial hydatidiform moles (Judson et al, 2002;Murdoch et al, 2006) result in abnormal placentation. Finally, interspecific hybrids (Vrana et al, 1998;Vrana et al, 2000), somatic cell nuclear transfer embryos (Dindot et al, 2004;Inoue et al, 2002) and uniparental embryos (Surani et al, 1986;Varmuza et al, 1993;Mann 2005) all suffer from dysmorphic trophoblast, in part or in whole because of abnormal expression of imprinted genes.…”

Section: Sfmbt2 and Yy1 Interact In Mammalian Cellsmentioning

confidence: 99%

The PcG gene Sfmbt2 is paternally expressed in extraembryonic tissues

Kuzmin

Han

Golding

et al. 2008

Gene Expression Patterns

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Genomic imprinting has dramatic effects on placental development, as has been clearly observed in interspecific hybrid, somatic cell nuclear transfer, and uniparental embryos. In fact, the earliest defects in uniparental embryos are evident first in the extraembryonic trophoblast. We performed a microarray comparison of gynogenetic and androgenetic mouse blastocysts, which are predisposed to placental pathologies, to identify imprinted genes. In addition to identifying a large number of known imprinted genes, we discovered that the Polycomb group (PcG) gene Sfmbt2 is imprinted. Sfmbt2 is expressed preferentially from the paternal allele in early embryos, and in later stage extraembryonic tissues. A CpG island spanning the transcriptional start site is differentially methylated on the maternal allele in e14.5 placenta. Sfmbt2 is located on proximal chromosome 2, in a region known to be imprinted, but for which no genes had been identified until now. This possibly identifies a new imprinted domain within the murine genome. We further demonstrate that murine SFMBT2 protein interacts with the transcription factor YY1, similar to the Drosophila PHO-RC. KeywordsGenomic Imprinting; Sfmbt2; PcG gene; Extraembryonic tissues; placenta; MMU2; YY1; microarrays; uniparental embryos Results and DiscusssionThe role played by imprinted genes in placental development is gaining wider attention as the impact of imprinting disruptions on placental pathology is explored. Both hyper-and hypoplastic placentation are linked to imprinted gene function (Kanayama et al., 2002;Murdoch et al., 2006; reviewed in Kaneko-Ishino et al., 2003). The most dramatic example of this is diploid, uniparental embryos. Gynogenetic embryos (two maternal/no paternal genomes) and androgenetic embryos (two paternal/no maternal genomes) both exhibit defects in the earliest differentiated tissue in mammals, the extraembryonic trophoblast (Barton et al., 1984;McGrath and Solter, 1984;Mann, 2005). At mid-gestation, gynogenetic embryos possess only a few residual trophoblast giant cells while androgenetic embryos are characterized by a mass of hypertrophic trophoblast. These observations indicate that a suite of genes required * Corresponding author. s.varmuza@utoronto.ca; tel. 1-416-978-2759; FAX 1-416-978-8532. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptGene Expr Patterns. Author manuscript; available in PMC 2009 January 1. Published in final edited form as:Gene Expr Patterns. 2008 January ; 8(2): 107-116. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manu...

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“…Disruption of imprinting in interspecific mammalian hybrids of the deermouse, Peromyscus maniculatus, may be due to nuclearcytoplasmic incompatibility 48 . Furthermore, certain oocyte cytoplasmic modifiers can induce epigenetic modifications of target loci, subsequently rendering them inactive by DNA methylation 49 .…”

Section: Genomic Reprogramming In the Zygotementioning

confidence: 99%

Reprogramming of genome function through epigenetic inheritance

Surani

2001

Nature

411

296

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Most cells contain the same set of genes and yet they are extremely diverse in appearance and functions. It is the selective expression and repression of genes that determines the specific properties of individual cells. Nevertheless, even when fully differentiated, any cell can potentially be reprogrammed back to totipotency, which in turn results in re-differentiation of the full repertoire of adult cells from a single original cell of any kind. Mechanisms that regulate this exceptional genomic plasticity and the state of totipotency are being unravelled, and will enhance our ability to manipulate stem cells for therapeutic purposes.

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Genomic imprinting is disrupted in interspecific Peromyscus hybrids

Cited by 170 publications

References 27 publications

Proliferation and growth factor expression in abnormally enlarged placentas of mouse interspecific hybrids

Proliferation and growth factor expression in abnormally enlarged placentas of mouse interspecific hybrids

The PcG gene Sfmbt2 is paternally expressed in extraembryonic tissues

Reprogramming of genome function through epigenetic inheritance

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