Genomic identification, expression profiling, and functional characterization of CatSper channels in the bovine†

Johnson, Gillian P.; English, A.M.; Cronin, Sinéad; Hoey, David A.; Meade, Kieran G.; Fair, Seán

doi:10.1093/biolre/iox082

Cited by 25 publications

(28 citation statements)

References 32 publications

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“…It demonstrated (i) that stallion sperm respond to a fluid flow and also that their rheotactic response is affected by fluid viscosity (ii) it clearly illustrates for the first time that hyperactivated stallion sperm do not exhibit a rheotaxis-like behaviour and that their swimming pattern against a fluid flow is uncoordinated and non-progressive, similar to the erratic pattern traditionally observed in a static environment. In contrast, this study showed (iii) that both hyperactivated human and ram sperm do indeed display positive a rheotaxis-like behaviour, however, hyperactivated motility did not affect their rate of progression as it does in bull sperm 29 .…”

Section: Discussioncontrasting

confidence: 81%

“…Although rheotaxis determination was not validated by a directionality method such as videomicroscopy, as described in other species 4 , 30 , 31 , this study has clearly shown that stallion sperm actively respond and adapt to fluid flow similar to human 4 and bull 29 and is in agreement with reported differences in the percentage of sperm exhibiting rheotaxis at different flow velocities in a microfluidic channel 25 . The only known published data on the rate of fluid flow in vivo is that by Miki and Clapham 4 who found the velocity of fluid flow within the oviduct of the mouse to be approximately 18 µm/s.…”

Section: Discussionsupporting

confidence: 80%

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Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species

Romero-Aguirregomezcorta

Sugrue

Martínez-Fresneda

et al. 2018

Sci Rep

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The journey of spermatozoa through the female genital tract is facilitated by rheotaxis, or the cell’s preference to swim against a flow, as well as thigmotaxis, the wall tracking behaviour, which guides them to the site of fertilisation. The aim of this study was to characterise the rheotactic and thigmotactic response of stallion sperm within a microfluidic channel. Stallion sperm rheotaxis was assessed within the microfluidic channel with regard to: (i) A range of flow velocities, (ii) Varying media viscosity and (iii) Sperm hyperactivation. Sperm distribution across the microfluidic channel was also studied and compared to human and ram sperm. Stallion sperm progressed furthest at a velocity range of 10–30 µm/s, with an optimum velocity of 20 µm/s. A flow viscosity of 2.5cP or greater reduced sperm rheotaxis (P < 0.05). Stallion sperm that were hyperactivated were unable to exhibit rheotaxis within the microfluidic channel, whereas, both hyperactivated human and ram sperm did exhibit positive rheotaxis under the same conditions. The number of sperm swimming near the microfluidic channel walls was higher than in the microfluidic channel centre (P < 0.05). This is the first study to illustrate that stallion sperm are rheotactically responsive and increasing viscosity reduces this response. We also demonstrated that sperm are predominantly inclined to swim along a surface and uniquely, hyperactivated stallion sperm are non-progressive and do not exhibit a rheotactic response unlike other species.

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Section: Discussioncontrasting

confidence: 81%

Section: Discussionsupporting

confidence: 80%

Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species

Romero-Aguirregomezcorta

Sugrue

Martínez-Fresneda

et al. 2018

Sci Rep

Self Cite

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“…Epididymal spermatozoa from male CatSper ‐KO mice are incapable of exhibiting hyperactivation by incubation in the capacitation‐supporting medium . Although the CatSper channels exist in bull and boar ejaculated spermatozoa, further experiments are necessary to demonstrate their functions in the occurrence of hyperactivation.…”

Section: Regulatory Mechanisms For Flagellar Hyperactivationmentioning

confidence: 99%

“…Epididymal spermatozoa from male CatSper-KO mice are incapable of exhibiting hyperactivation by incubation in the capacitation-supporting medium 64,66,67. Although the CatSper channels exist in bull and boar ejaculated spermatozoa,68,69 further experiments are necessary to demonstrate their functions in the occurrence of hyperactivation.In bulls, boars, and mice, hyperactivation can be induced immediately in uncapacitated spermatozoa by a short treatment with putative stimulator for IP 3 R "thimerosal." The thimerosal-induced hyperactivation is full-type (bulls and boars)/anti-hock (mice) 38,50,51,70.…”

mentioning

confidence: 99%

Flagellar hyperactivation of bull and boar spermatozoa

Harayama

2018

Reprod Medicine & Biology

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BackgroundIn mammals, flagellar hyperactivation is indispensable to sperm fertilization with oocytes in vivo, although there are species differences in regulatory mechanisms for this event. In this study, I reviewed researches regarding hyperactivation of bull and boar spermatozoa, in comparison with those of spermatozoa from other species.MethodsRecent publications regarding sperm hyperactivation were collected and summarized.Results (Main findings)In bull and boar spermatozoa, there are two types of hyperactivation “full‐type hyperactivation and nonfull‐type hyperactivation” which are equivalent to anti‐hock hyperactivation and pro‐hock hyperactivation of mouse spermatozoa, respectively, on the basis of the flagellar parts exhibiting asymmetrical beating. Full‐type hyperactivation is initiated in response to a rapid increase of cytoplasmic Ca2+ in the connecting/middle and principal pieces by the mobilization of this divalent ion from extracellular space and internal store through cation channels. Regulatory molecules for the increase of cytoplasmic Ca2+ in the connecting/middle pieces are probably different from those in the principal pieces.ConclusionI have proposed a hypothesis on the regulation of full‐type hyperactivation by the distinct signaling cascades leading to the increase in cytoplasmic Ca2+ between the connecting/middle and principal pieces of bull and boar spermatozoa.

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“…CatSper glycoproteins form the sperm-specific voltage-gated Ca 2+ channels localised along the membrane of the sperm flagellum. CatSper channels contain glycoproteins that are involved in positioning regulation and recent work by our group has demonstrated that hyperactive bull sperm exhibit an increased rheotaxis response (Johnson et al, 2017). Targeted disruption of CATSPER 1, CATSPER2, CATSPER3 or CAT-SPER4 inhibits hyperactivated motility and thus rheotactic reponse (Johnson et al, 2017).…”

Section: Sperm Transport In the Female Tractmentioning

confidence: 99%

Review: Understanding the causes of variation in reproductive wastage among bulls

Fair

Lonergan

2018

Animal

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The ability to predict the fertility of bulls before semen is released into the field has been a long-term objective of the animal breeding industry. However, the recent shift in the dairy industry towards the intensive use of young genomically selected bulls has increased its urgency. Such bulls, which are often in the highest demand, are frequently only used intensively for one season and consequently there is limited time to track their field fertility. A more pressing issue is that they produce fewer sperm per ejaculate than mature bulls and therefore there is a need to reduce the sperm number per straw to the minimum required without a concomitant reduction in fertility. However, as individual bulls vary in the minimum number of sperm required to achieve their maximum fertility, this cannot be currently achieved without extensive field-testing. Although an in vitro semen quality test, or combination of tests, which can accurately and consistently determine a bull's fertility and the optimum sperm number required represent the 'holy grail' in terms of semen assessment, this has not been achieved to date. Understanding the underlying causes of variation in bull fertility is a key prerequisite to achieving this goal. In this review, we consider the reliability of sire conception rate estimates and then consider where along the pregnancy establishment axis the variation in reproductive loss between bulls occurs. We discuss the aetiology of these deficiencies in sperm function and propose avenues for future investigation.

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Genomic identification, expression profiling, and functional characterization of CatSper channels in the bovine†

Cited by 25 publications

References 32 publications

Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species

Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species

Flagellar hyperactivation of bull and boar spermatozoa

Review: Understanding the causes of variation in reproductive wastage among bulls

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