Genomic heterogeneity and host recovery of isolates of Malva vein clearing virus

Lunello, Pablo; Touriño, Angeles; Núñez, Yolanda; Ponz, F; Sánchez, Flora

doi:10.1016/j.virusres.2008.11.006

Cited by 6 publications

(3 citation statements)

References 27 publications

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“…MVCV first appeared in mallows in Germany, which causes mosaic and pulse symptoms. Subsequently, it was reported in A. rosea in Spain ( Lunello et al, 2009 ), Germany ( Menzel et al, 2010 ), Hungary and Yugoslavia ( Horváth et al, 2010 ), Italy ( Parrella et al, 2015 ), and China ( Yang et al, 2017 ). Therefore, MVCV is considered a worldwide plant viral disease that causes mosaic and vein-clearing symptoms ( Hein, 1959 ).…”

mentioning

confidence: 99%

Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing

et al. 2020

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mentioning

confidence: 99%

Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing

et al. 2020

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“…MVCV (PV-0963, GQ856544) (C. quinoa) (Menzel , 2010). (MVCV-01-06) (Ds-Ba-01, Napoli) MVCV (Lunello , 2009;Parrella , 2015).…”

Section: Cm3 (Chlorotic Local)mentioning

confidence: 99%

Characterization of Three Korean Isolates of Malva Vein Clearing Virus from Curled Mallow (Malva verticillata)

곽¹,

김²,

김³

et al. 2020

Res. Plant Dis

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In September 2017, vein clearing and yellowing symptoms resembling those caused by viruses were observed on leaves of Malva verticillata in Chungnam, Korea. Nucleic acids were extracted from leaves of five symptomatic plants and tested by reverse transcription polymerase chain reaction using four virus specific primer pairs including malva vein clearing virus (MVCV). Amplicons of the expected size (600 bp) were obtained from total RNA of all samples using the MVCV-specific primers. To confirm the presence of MVCV in symptomatic plants, the DNA fragments from three samples were purified, and directly sequenced. BLAST analysis revealed that it shared the highest nucleotide identity (99%) with a MVCV isolate from tomato (Mexico). The virus isolates obtained from the third re-inoculated Chenopodium was designated as Cm1-5. Tissue from Cm1, Cm3, and Cm5 isolates was mechanically sap inoculated into 23 indicator plants. Cm3 isolate induced chlorotic local and mosaic symptoms in Althaea rosea. Phylogenetic analysis based on coat protein gene of 19 MVCV isolates from 6 different countries and plant species, did not correlated with either the geographical origin of the isolates, or pathogenicity. To our knowledge, this study first reports the natural occurrence of MVCV on M. verticillata in Korea and characterization of three Korean isolates of MVCV.

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“…Malva spp. are reported to be affected by several viral agents including malva vein clearing virus (MVCV) (Lunello et al, 2009), watermelon mosaic virus (Kim et al, 2019), cherry rasp leaf virus (Villamor and Eastwell, 2016), cucumber vein yellowing virus (Janssen et al, 2002), and tomato leaf curl Kerala virus associated with yellow vein net disease (Snehi et al, 2018). These viruses have a significant negative impact on malva plants provoking a wide range of symptoms, reducing both ornamental and economic values.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Analysis of Malva sylvestris from Iran Displays a Malva Vein Clearing Virus Genome

Moradi

Mehrvar²

2023

JAST

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To date, only the complete genome of one malva vein clearing virus (MVCV) has been sequenced worldwide. Here, for the first time, the genomic sequence of an isolate of MVCV affecting Malva sylvestris in Iran was determined, using RNA deep sequencing confirmed by reverse-transcription polymerase chain reaction and Sanger sequencing. The sequence of IR1 was 11,055 nucleotides in length and contained a single open reading frame of 10,527 nucleotides encoding a large polyprotein of 3,508 amino acids with predicted molecular weight of 395,08 KDa. The sequence contained nine putative proteolytic cleavage sites and motifs conserved in homologous proteins of other potyviruses. The P1 of the IR1 was 1236 nucleotides longer than that of the only recently reported sequence from China (MVCV-SX, MN116683). The complete genome sequence obtained from the study showed 81.24% and 91.93% identities to its Chinese counterpart at the nucleotide (nt) and deduced amino acid (aa) levels, respectively. The low nt and aa sequence identity with known MVCV isolate seems to indicate that IR1 is a novel strain. Phylogenetic analysis of the coat protein gene also showed that the Iranian isolate was most closely related to the Dutch isolate NAKT-NL (FJ539084), with identities of 94.06 (at nt level) and 96.04% (at aa level). The results of this study will be useful for understanding the global molecular epidemiology of MVCV.

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Genomic heterogeneity and host recovery of isolates of Malva vein clearing virus

Cited by 6 publications

References 27 publications

Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing

Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing

Characterization of Three Korean Isolates of Malva Vein Clearing Virus from Curled Mallow (Malva verticillata)

Metagenomic Analysis of Malva sylvestris from Iran Displays a Malva Vein Clearing Virus Genome

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