Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis

Belfaiza, J; Postić, D.; Bellenger, E.; Baranton, Guy; Girons, Isabelle Saint

doi:10.1128/jcm.31.11.2873-2877.1993

Cited by 102 publications

(50 citation statements)

References 28 publications

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“…Earlier studies on PFGE analysis of the Borrelia genome demonstrated their usefulness in identifying isolates, and a species identified by PFGE exactly corresponded to that obtained previously with other methods (6). We used PFGE analysis to identify several Japanese borrelial isolates and the results are presented in this paper.…”

supporting

confidence: 59%

“…Pulsed field gel electrophoresis ( PFGE) is a newly developed tool for characterizing large restriction fragments produced by rare-cutter restriction enzymes. Earlier studies revealed that this method was successfully used to construct a physical map of the B. burgdorferi genome and is also useful in identifying geographical isolates as belonging to a specific species and for epidemiological studies (5,6). We analyzed several B. burgdorferi sensu lato isolates by PFGE.…”

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confidence: 99%

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Pulsed Field Gel Electrophoresis Analysis of Borrelia burgdorferi Sensu Lato Isolated in Japan and Taxonomic Implications with Lyme Disease Spirochetes

Fukunaga

Takahashi

1994

Microbiology and Immunology

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Genomic DNAs of Borrelia burgdorferi sensu lato isolates obtained in Japan sharing different rRNA gene ribotypes were digested with rare-cutter restriction endonucleases and the fragments obtained were separated by pulsed field gel electrophoresis (PFGE). The sizes of large restriction cleavage bands with Mlul endonuclease were quite similar among isolates in each ribotype group. On the other hand, the PFGE profiles obtained with the other enzymes (Nrul, Sal I or SplI) were rather divergent, and Japanese isolates were distinguishable from the United States and European isolates. The Japanese isolates classified as ribotypes group II (Borrelia garinii) and III (B. afzelii) showed different PFGE patterns from that of European isolates. The isolates grouped into ribotype IV revealed distinctively different PFGE profiles. These results indicate that the Japanese isolates may be genetically divergent and distinct from the United States and European isolates.

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confidence: 59%

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confidence: 99%

Pulsed Field Gel Electrophoresis Analysis of Borrelia burgdorferi Sensu Lato Isolated in Japan and Taxonomic Implications with Lyme Disease Spirochetes

Fukunaga

Takahashi

1994

Microbiology and Immunology

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“…Genomic fingerprinting of B. burgdorferi sensu lato strains by pulsed-field gel electrophoresis (PFGE) showed that all isolates used in this study were recognized by one band (135 kbp), each of the B. garinii isolates by two bands (220 and 80 kbp), and each of the B. afzelii isolates by three bands (460, 320 and 90 kbp). Whilst there were differences in the PFGE patterns among B. burgdorferi and B. garinii isolates, the patterns of B. afzelii isolates were all similar [15]. The number of genomic species was further expanded by the characterization of borreliae isolated from Ixodes ovatus ticks in Japan.…”

Section: Expansion Of the Lyme Borrelia Complex During The 1990smentioning

confidence: 99%

The expanding Lyme Borrelia complex—clinical significance of genomic species?

Stanek¹,

Reiter²

2011

Clinical Microbiology and Infection

268

168

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Ten years after the discovery of spirochaetes as agents of Lyme disease in 1982 in the USA, three genomic species had diverged from the phenotypically heterogeneous strains of Borrelia burgdorferi isolated in North America and Europe: Borrelia afzelii, B. burgdorferi sensu stricto (further B. burgdorferi), and Borrelia garinii. Whereas B. burgdorferi remained the only human pathogen in North America, all three species are aetiological agents of Lyme borreliosis in Europe. Another seven genospecies were described in the 1990s, including species from Asia (Borrelia japonica, Borrelia turdi, and B. tanukii), North America (Borrelia andersonii), Europe (Borrelia lusitaniae and Borrelia valaisiana), and from Europe and Asia (Borrelia bissettii). Another eight species were delineated in the years up to 2010: Borrelia sinica (Asia), Borrelia spielmanii (Europe), Borrelia yangtze (Asia), Borrelia californiensis, Borrelia americana, Borrelia carolinensis (North America), Borrelia bavariensis (Europe), and Borrelia kurtenbachii (North America). Of these 18 genomic species B. afzelii, B. burgdorferi and B. garinii are the confirmed agents of localized, disseminated and chronic manifestations of Lyme borreliosis, whereas B. spielmanii has been detected in early skin disease, and B. bissettii and B. valaisiana have been detected in specimens from single cases of Lyme borreliosis. The clinical role of B. lusitaniae remains to be substantiated.

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“…Lyme borreliosis, the most common tick-borne infection in the Northern hemisphere, is caused by the spirochete Borrelia burgdorferi sensu lato (1). At least three species have been associated with human Lyme borreliosis: B. afzelii, B. garinii and B. burgdorferi sensu stricto (2). The disease manifestations and the outcome seem to depend at least partly on the nature of the infecting organism (3,4).…”

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Analysis of Borrelia burgdorferi sensu lato isolated from cerebrospinal fluid^Note

Ružić‐Sabljić

Lotrič‐Furlan

Maraspin

et al. 2001

APMIS

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Involvement of the nervous system in Lyme borreliosis may occur with or without erythema migrans and it may present with a variety of neurological symptoms. In this study we analysed phenotypic and genotypic characteristics of 40 Borrelia strains isolated from cerebrospinal fluid (CSF) of 38 Slovenian patients with different clinical manifestations of Lyme borreliosis. In seven of the patients, Borreliae were also isolated from skin lesions. Species identification and plasmid profiles were determined by pulsed-field gel electrophoresis and protein profiles by SDS-PAGE. MluI digestion profiles of Borrelia burgdorferi sensu lato DNA showed that 25 (62.5%) isolates were B. garinii, 14 (35%) B. afzelii, and one (2.5%) B. burgdorferi sensu stricto. All strains, except one, possessed a large plasmid and a varying number of smaller plasmids. Three (7.5%) isolates exhibited an unusual plasmid profile, with a large plasmid dimer or three copies of the large plasmid. In protein analyses, all strains expressed OspA protein. OspB was present significantly more often in B. afzelii than B. garinii strains (p=0.0000), while OspC was more often present in B. garinii than B. afzelii strains (p=0.0052). In the seven patients with Borreliae isolated also from the skin, the CSF and skin isolates were identical, either B. garinii (six patients) or B. afzelii (one patient). Species and plasmid heterogeneity as well as antigen diversity could play a role in the pathogenesis of the infection. When combined with our own earlier data, the results suggest species-related organotropism.

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Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis

Cited by 102 publications

References 28 publications

Pulsed Field Gel Electrophoresis Analysis of Borrelia burgdorferi Sensu Lato Isolated in Japan and Taxonomic Implications with Lyme Disease Spirochetes

Pulsed Field Gel Electrophoresis Analysis of Borrelia burgdorferi Sensu Lato Isolated in Japan and Taxonomic Implications with Lyme Disease Spirochetes

The expanding Lyme Borrelia complex—clinical significance of genomic species?

Analysis of Borrelia burgdorferi sensu lato isolated from cerebrospinal fluid^Note

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Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis

Cited by 102 publications

References 28 publications

Pulsed Field Gel Electrophoresis Analysis of Borrelia burgdorferi Sensu Lato Isolated in Japan and Taxonomic Implications with Lyme Disease Spirochetes

Pulsed Field Gel Electrophoresis Analysis of Borrelia burgdorferi Sensu Lato Isolated in Japan and Taxonomic Implications with Lyme Disease Spirochetes

The expanding Lyme Borrelia complex—clinical significance of genomic species?

Analysis of Borrelia burgdorferi sensu lato isolated from cerebrospinal fluidNote

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Analysis of Borrelia burgdorferi sensu lato isolated from cerebrospinal fluid^Note