Genomic features of the polyphagous cotton leafworm Spodoptera littoralis

Wu, Chao; Zhang, Lei; Liu, Bo; Gao, Bojia; Huang, Cong; Zhang, Ji; Jin, Minghui; Wang, Hanyue; Peng, Yan; Rice, Annabel; Hegazi, E. M.; Wilson, Kenneth; Xu, Pengjun; Xiao, Yutao

doi:10.1186/s12864-022-08582-w

Cited by 4 publications

(1 citation statement)

References 55 publications

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“…Hence, extensive research about the genomics and functional genomics is required to develop effective management strategies. To date, more than 30 genomic sequences of lepidopteran species have been obtained [1,5]. Genome editing has been achieved in a few lepidopteran insects.…”

Section: Introductionmentioning

confidence: 99%

Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in Bombyx mori

Yu,

Dong,

Jiang

et al. 2023

Insects

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Lepidoptera is one of the most speciose insect orders, causing enormous damage to agricultural and forest crops. Although genome editing has been achieved in a few Lepidoptera for insect controls, most techniques are still limited. Here, by injecting female pupae of the Lepidoptera model species, Bombyx mori, gene editing was established using the Receptor-Mediated Ovary Transduction of Cargo (ReMOT) control technique. We identified a B. mori oocytes-targeting peptide ligand (BmOTP, a 29 aa of vitellogenin N-terminal of silkworms) with a highly conserved sequence in lepidopteran insects that could efficiently deliver mCherry into oocytes. When BmOTP was fused to CRISPR-associated protein 9 (Cas9) and the BmOTP-Cas9 ribonucleoprotein complex was injected into female pupae, heritable editing of the offspring was achieved in the silkworms. Compared with embryo microinjection, individual injection is more convenient and eliminates the challenge of injecting extremely small embryos. Our results will significantly facilitate the genetic manipulation of other lepidopteran insects, which is essential for advancing lepidopteran pest control.

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Section: Introductionmentioning

confidence: 99%

Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in Bombyx mori

Yu,

Dong,

Jiang

et al. 2023

Insects

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Chromosome‐level genome assembly and comparative genomics shed light on Helicoverpa assulta ecology and pest management

Kim,

Rahman,

Han

et al. 2024

Pest Management Science

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BACKGROUNDThe Oriental tobacco budworm, Helicoverpa assulta, a specialist herbivorous insect that exclusively feeds on plants of the Solanaceae family, causes considerable damage to crops, such as tobacco and hot pepper. The absence of a genome sequence for this species hinders further research on its pest management and ecological adaptation.RESULTSHere, we present a high‐quality chromosome‐level genome of a Korean strain of H. assulta (Pyeongchang strain, K18). The total assembly spans 424.4 Mb with an N50 length of 14.54 Mb and 37% GC content. The assembled genome (ASM2961881v1) comprises 31 chromosomes, similar to other congeneric generalist species including H. armigera and H. zea. In terms of genomic assembly quality, the complete BUSCOs and repeat content accounted for 98.3% and 33.01% of the genome, respectively. Based on this assembly, 19 485 protein‐coding genes were predicted in the genome annotation. A comparative analysis was conducted using the identified number of protein‐coding genes in H. armigera (24154) and H. zea (23696). Out of the 19 485 predicted genes, 137 genes in 15 orthogroups were found to have expanded significantly in H. assulta, while 149 genes in 95 orthogroups contracted rapidly.CONCLUSIONThis study revealed specific gene expansions and contractions in H. assulta compared to those in its close relatives, indicating potential adaptations related to its specialized feeding habits. Also, the comparative genome analysis provides valuable insights for the integrated pest management of H. assulta and other globally significant pests in the Heliothinae subfamily. © 2024 Society of Chemical Industry.

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Exaptation of I4760M mutation in ryanodine receptor of Spodoptera exigua (Lepidoptera: Noctuidae): Lessons from museum and field samples

Han,

Rahman,

Shin

et al. 2023

Pesticide Biochemistry and Physiology

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Genomic features of the polyphagous cotton leafworm Spodoptera littoralis

Cited by 4 publications

References 55 publications

Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in Bombyx mori

Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in Bombyx mori

Chromosome‐level genome assembly and comparative genomics shed light on Helicoverpa assulta ecology and pest management

Exaptation of I4760M mutation in ryanodine receptor of Spodoptera exigua (Lepidoptera: Noctuidae): Lessons from museum and field samples

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