Genomic expression profiles in liver of mice exposed to purified terephthalic acid manufacturing wastewater

Zhang, Zongyao; Ma, Liping; Zhang, Xuxiang; Li, Weixin; Zhang, Yan; Wu, Bing; Yang, Liuyan; Cheng, Shengfeng

doi:10.1016/j.jhazmat.2010.05.131

Cited by 18 publications

(7 citation statements)

References 35 publications

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“…To investigate the histopathological changes in the tissues of MWWEtreated mice, livers and kidneys were fixed in 10% formalin for 24 h. Histopathological assessments were performed following the standard procedures. 20 In brief, the fixed tissues were embedded in paraffin wax, sectioned at 5-μm thickness, stained with hematoxylin and eosin (HE), and finally observed under an optical microscope. Observers remained blinded to treatment/control group assignments for histopathology observation (10 tissue samples for each group and 5 slices for each tissue).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Evaluation of the Toxic Effects of Municipal Wastewater Effluent on Mice Using Omic Approaches

Zhang

Huang

Deng

et al. 2013

Environ. Sci. Technol.

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Municipal wastewater effluents (MWWE) contain a lot of trace organic pollutants, which will be a threat to environmental health. However, little information is available for the mixed toxicity of MWWE on mammals. In the present study, male mice were exposed to MWWE for 90 days, and then, histopathology and clinical biochemistry determination and transcriptomic and metabolomic profiling were conducted. The results showed that MWWE exposure resulted in injuries in liver and kidney. Combined transcriptomic and metabolomic data demonstrated that MWWE exposure induced perturbations of metabolism, including lipid, nucleotide, amino acid, and energy metabolism. Furthermore, dysregulation of signal transduction processes were also identified based on differentially expressed genes. These results suggested that chronic exposure to MWWE could induce hepatotoxicity and nephrotoxicity in mice and omic approaches are of practical value to evaluate the complex toxicity of MWWE.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Evaluation of the Toxic Effects of Municipal Wastewater Effluent on Mice Using Omic Approaches

Zhang

Huang

Deng

et al. 2013

Environ. Sci. Technol.

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“…In order to compare the alterations of the genes expressions, the treated and controlled samples were hybridized separately into a total of nine genechips (the microarrays were conducted in triplicate for each group). Details about the microarray detection were previously described by Zhang et al (2010). After hybridization, the cocktail was removed and the arrays were washed and stained in Genechip_Fluidics Station 450 (Affymetrix, CA, USA) and scanned on the Genechip_Scanner 3000 7G (Affymetrix, CA, USA).…”

Section: Genechipmentioning

confidence: 99%

“…DNA microarray is considered as a promising genomic profiling technique characterized with high throughput, high speed and high sensitivity, which has been used to evaluate the toxicities of environmental samples, including source water ), drinking water (Shi et al 2012), industrial effluent (Zhang et al 2010) and sediments (Kosmehl et al 2012). In this study, we used DNA microarray to evaluate hepatic genomic toxicity on mice sub-chronically exposed to source water (NJS) and tap water (NJT) from Yangtze River, focusing on exploring the potential molecular pathways mediating the lipid metabolism disturbance.…”

Section: Introductionmentioning

confidence: 99%

A transcriptomics study on hepatic lipid metabolism in mice exposed to contaminated drinking water

Yin

Zhao

Zhang

et al. 2013

Int. J. Environ. Sci. Technol.

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DNA microarray was used to analyze hepatic transcriptional profile of male mice (Mus musculus) after the mice were fed with Yangtze River (China) source water (NJS) and tap water (NJT) for 90 days. Chemical analysis demonstrated that NJS and NJT contained various tracelevel pollutants including polycyclic aromatic hydrocarbons, phthalate ethers and inorganic contaminants. DNA microarray revealed occurrence of 5,042 differentially expressed genes (DEGs) in the mice fed with NJS and 828 DEGs in the mice fed with NJT, indicating that NJS posed greater influence on liver transcriptome. Annotation against Kyoto Encyclopedia of Genes and Genomes pathway database showed that the DEGs in NJS group were mostly involved in lipid metabolism (51 DEGs), followed by neurodegenerative diseases (47 DEGs), energy metabolism (41 DEGs) and endocrine system (38 DEGs). NJT exposure was found to affect lipid metabolism (14 DEGs), xenobiotics biodegradation and metabolism (6 DEGs), and cofactors and vitamins metabolism (5 DEGs). Annotation against Gene Ontology database confirmed that lipid metabolism among the altered pathways was most susceptible to both NJS and NJT exposure. The DEGs were involved in 6 lipid metabolic pathways including fatty acid metabolism, glycerophospholipid metabolism, unsaturated fatty acid biosynthesis, steroid biosynthesis, primary bile acid biosynthesis and steroid hormone biosynthesis. Although both NJS and NJT might cause no obvious liver tissue damages, the lipid metabolic disturbance induced by trace-level pollutants still deserves public health concern.

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“…Wastewater from a purified terephthalic acid manufacturing plant was found to have reproductive toxicity (Zhang, Sun et al, 2010), as well as hepatotoxicity (Zhang, Ma et al, 2010) in male mice (Mus musculus).…”

Section: Sdirimentioning

confidence: 99%

Health Effects Associated with Wastewater Treatment, Reuse, and Disposal

Peterson¹,

Murphy²,

Ye³

et al. 2011

Water Environment Research

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Genomic expression profiles in liver of mice exposed to purified terephthalic acid manufacturing wastewater

Cited by 18 publications

References 35 publications

Evaluation of the Toxic Effects of Municipal Wastewater Effluent on Mice Using Omic Approaches

Evaluation of the Toxic Effects of Municipal Wastewater Effluent on Mice Using Omic Approaches

A transcriptomics study on hepatic lipid metabolism in mice exposed to contaminated drinking water

Health Effects Associated with Wastewater Treatment, Reuse, and Disposal

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