Genomic DNA detection using cycling probe technology and capillary gel electrophoresis with laser-induced fluorescence

Laing, T D; Mah, David; Poirier, Robert T.; Bekkaoui, Faouzi; Lee, William; Bader, Douglas E.

doi:10.1016/j.mcp.2004.06.001

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Cited by 6 publications

(1 citation statement)

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“…rescent label. This approach has been used to develop an assay for cleavable nucleic acid probes using CE-LIF [17]. However, preliminary studies for this work suggested that the peptidase activity of the BoNT/A light chain was sensitive to end-located substituents such as fluorescein dye.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis laser-induced fluorescence for screening combinatorial peptide libraries in assays of botulinum neurotoxin A

Laing

Marenco

Moore³

et al. 2006

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis laser-induced fluorescence for screening combinatorial peptide libraries in assays of botulinum neurotoxin A

Laing

Marenco

Moore³

et al. 2006

Journal of Chromatography B

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Probe Amplification Technologies

Cui

Feng

et al. 2012

Advanced Techniques in Diagnostic Microbiology

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Enzyme-assisted target recycling (EATR) for nucleic acid detection

2014

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Fast, reliable and sensitive methods for nucleic acid detection are of growing practical interest with respect to molecular diagnostics of cancer, infectious and genetic diseases. Currently, PCR-based and other target amplification strategies are most extensively used in practice. At the same time, such assays have limitations that can be overcome by alternative approaches. There is a recent explosion in the design of methods that amplify the signal produced by a nucleic acid target, without changing its copy number. This review aims at systematization and critical analysis of the enzyme-assisted target recycling (EATR) signal amplification technique. The approach uses nucleases to recognize and cleave the probe-target complex. Cleavage reactions produce a detectable signal. The advantages of such techniques are potentially low sensitivity to contamination and lack of the requirement of a thermal cycler. Nucleases used for EATR include sequence-dependent restriction or nicking endonucleases or sequence independent exonuclease III, lambda exonuclease, RNase H, RNase HII, AP endonuclease, duplex-specific nuclease, DNase I, or T7 exonuclease. EATR-based assays are potentially useful for point-of-care diagnostics, single nucleotide polymorphisms genotyping and microRNA analysis. Specificity, limit of detection and the potential impact of EATR strategies on molecular diagnostics are discussed.

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Genomic DNA detection using cycling probe technology and capillary gel electrophoresis with laser-induced fluorescence

Cited by 6 publications

References 19 publications

Capillary electrophoresis laser-induced fluorescence for screening combinatorial peptide libraries in assays of botulinum neurotoxin A

Capillary electrophoresis laser-induced fluorescence for screening combinatorial peptide libraries in assays of botulinum neurotoxin A

Probe Amplification Technologies

Enzyme-assisted target recycling (EATR) for nucleic acid detection

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