Genomic diversity of resistant and virulent factors of Burkholderia pseudomallei clinical strains recovered from Guangdong using whole genome sequencing

Shafiq, Muhammad; Ke, Bixia; Li, Xin; Mi, Zeng; Yuan, Yumeng; He, Dongmei; Deng, Xiaoling; Jiao, Xiaoyang

doi:10.3389/fmicb.2022.980525

Cited by 3 publications

(1 citation statement)

References 56 publications

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“…Due to the lack of new antibiotics on the market, the emergence of MDR bacteria makes it increasingly difficult to prevent and control Salmonella infections. In addition, China, the United States, and the European Union have banned the use of multiple antibiotics in animal husbandry, making biosecurity more difficult and expensive to maintain at low risk levels on farms ( 14 , 15 ). Therefore, there is an urgent need for safe, effective, and low-cost means to control Salmonella infections in animal husbandry and the associated processes, including food, transportation, and storage.…”

Section: Introductionmentioning

confidence: 99%

Efficient screening of adsorbed receptors for Salmonella phage LP31 and identification of receptor-binding protein

Ge,

Ye,

Cai

et al. 2023

Microbiol Spectr

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The adsorption process is the first step in the lifecycle of phages and plays a decisive role in the entire infection process. Identifying the adsorption mechanism of phages not only makes phage therapy more precise and efficient but also enables the exploration of other potential applications and modifications of phages. Phage LP31 can lyse multiple Salmonella serotypes, efficiently clearing biofilms formed by Salmonella enterica serovar Enteritidis ( S . Enteritidis) and significantly reducing the concentration of S . Enteritidis in chicken feces. Therefore, LP31 has great potential for many practical applications. In this study, we established an efficient screening method for phage infection-related genes and identified a total of 10 genes related to the adsorption process of phage LP31. After the construction of strain C50041Δ rfaL 58−358 , it was found that the knockout strain had a rough phenotype as an O-antigen-deficient strain. Adsorption rate and transmission electron microscopy experiments showed that the receptor for phage LP31 was the O 9 antigen of S . Enteritidis. Homology comparison and adsorption experiments confirmed that the tail fiber protein Lp35 of phage LP31 participated in the adsorption process as a receptor-binding protein. IMPORTANCE A full understanding of the interaction between phages and their receptors can help with the development of phage-related products. Phages like LP31 with the tail fiber protein Lp35, or a closely related protein, have been reported to effectively recognize and infect multiple Salmonella serotypes. However, the role of these proteins in phage infection has not been previously described. In this study, we established an efficient screening method to detect phage adsorption to host receptors. We found that phage LP31 can utilize its tail fiber protein Lp35 to adsorb to the O 9 antigen of S. Enteritidis, initiating the infection process. This study provides a great model system for further studies of how a phage-encoded receptor-binding protein (RBP) interacts with its host's RBP binding target, and this new model offers opportunities for further theoretical and experimental studies to understand the infection mechanism of phages.

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Section: Introductionmentioning

confidence: 99%

Efficient screening of adsorbed receptors for Salmonella phage LP31 and identification of receptor-binding protein

Ge,

Ye,

Cai

et al. 2023

Microbiol Spectr

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Multi-Locus Sequence Typing and Genetic Diversity of Antibiotic-Resistant Genes and Virulence-Associated Genes in Burkholderia pseudomallei: Insights from Whole Genome Sequencing of Animal and Environmental Isolates in Thailand

Laklaeng,

Songsri,

Wisessombat

et al. 2024

Veterinary Microbiology

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Tree Visualization By One Table (tvBOT): a web application for visualizing, modifying and annotating phylogenetic trees

Xie

Chen

Cai

et al. 2023

Nucleic Acids Research

217

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tvBOT is a user-friendly and efficient web application for visualizing, modifying, and annotating phylogenetic trees. It is highly efficient in data preparation without requiring redundant style and syntax data. Tree annotations are powered by a data-driven engine that only requires practical data organized in uniform formats and saved as one table file. A layer manager is developed to manage annotation dataset layers, allowing the addition of a specific layer by selecting the columns of a corresponding annotation data file. Furthermore, tvBOT renders style adjustments in real-time and diversified ways. All style adjustments can be made on a highly interactive user interface and are available for mobile devices. The display engine allows the changes to be updated and rendered in real-time. In addition, tvBOT supports the combination display of 26 annotation dataset types to achieve multiple formats for tree annotations with reusable phylogenetic data. Besides several publication-ready graphics formats, JSON format can be exported to save the final drawing state and all related data, which can be shared with other users, uploaded to restore the final drawing state for re-editing or used as a style template for quickly retouching a new tree file. tvBOT is freely available at: https://www.chiplot.online/tvbot.html.

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Genomic diversity of resistant and virulent factors of Burkholderia pseudomallei clinical strains recovered from Guangdong using whole genome sequencing

Cited by 3 publications

References 56 publications

Efficient screening of adsorbed receptors for Salmonella phage LP31 and identification of receptor-binding protein

Efficient screening of adsorbed receptors for Salmonella phage LP31 and identification of receptor-binding protein

Multi-Locus Sequence Typing and Genetic Diversity of Antibiotic-Resistant Genes and Virulence-Associated Genes in Burkholderia pseudomallei: Insights from Whole Genome Sequencing of Animal and Environmental Isolates in Thailand

Tree Visualization By One Table (tvBOT): a web application for visualizing, modifying and annotating phylogenetic trees

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