Genomic Determinants of Entomopathogenic Fungi and Their Involvement in Pathogenesis

Vidhate, Ravindra P.; Dawkar, Vishal V.; Punekar, Sachin A.; Giri, Ashok P.

doi:10.1007/s00248-021-01936-z

Cited by 14 publications

(4 citation statements)

References 93 publications

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“…EPF can release lipase, chitinase, amylase, protease, and phosphatase (Harith-Fadzilah et al, 2021;Vidhate et al, 2023;Wang & Wang, 2017). Protease is the most crucial cuticle-degrading enzyme, and this enzyme's activity stimulates the chitinase enzyme's emergence.…”

Section: Pathogenicity Of B Bassiana Towards S Lituramentioning

confidence: 99%

Performance and Virulence of the Entomopathogenic Fungi Beauveria bassiana Grown in Media Derived from Biodegradable Agricultural Wastes Enriched with Cricket Powder

Afandhi,

Rachmawati,

Syib’li

et al. 2023

Agrivita.J.Agr.Sci

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Entomopathogenic fungi (EPF) can be an eco-friendly alternative to control pests. To produce large-scale EPF requires underused economical substrates containing high carbon and nitrogen; hence the production could be cost-effective. This study investigates the effects of organic agricultural waste, i.e., rice bran, rice husks, tea dregs, and wheat bran enriched with cricket powder as culture media on the mycelial growth, sporulation, and conidia viability of Beauveria bassiana and the fungal pathogenicity towards Spodoptera litura larvae. For each type of medium, five independent cultures of B. bassiana are treated as replications. B. bassiana grown on a PDA medium is treated as a control. This study shows that rice bran enriched with cricket powder became the most suitable waste medium to support the mass production of virulent B. bassiana. Therefore, rice bran media enriched with cricket powder can be used as an appropriate medium for the mass production of B. bassiana.

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Section: Pathogenicity Of B Bassiana Towards S Lituramentioning

confidence: 99%

Performance and Virulence of the Entomopathogenic Fungi Beauveria bassiana Grown in Media Derived from Biodegradable Agricultural Wastes Enriched with Cricket Powder

Afandhi,

Rachmawati,

Syib’li

et al. 2023

Agrivita.J.Agr.Sci

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“…The insect's mortality caused by using entomopathogenic fungus happens due to the presence of poisonous chemicals and enzymes in their host. This fungus is also able to produce protease, chitinase, lipase, carboxypeptidases, and phosphatase (Harith-Fadzilah et al, 2021;Vidhate et al, 2023;Wang et al, 2021).…”

Section: Adult Emergence Ratio Of Cocoa Pod Borermentioning

confidence: 99%

“…The initial phase in fungal entomopathogenic infection is the insect's cuticle layer penetration by the enzymes and hyphae-secreted specialized infection structures (appressoria) that infiltrate the host cells (Wang et al, 2021). Protease degrades the cuticle's outer layer proteins, enabling chitinase to penetrate the pro-cuticular chitin (Vidhate et al, 2023;Włóka et al, 2022) and hyphae to reach the insect hemolymphoid (Wang et al, 2021). Moreover, lipase breaks down lipoproteins, lipids, and wax layers, which are then utilized as a source of nutrients by the fungus (Włóka et al, 2022).…”

Section: Adult Emergence Ratio Of Cocoa Pod Borermentioning

confidence: 99%

The effect of chitin on the effectiveness of Beauveria bassiana formulation product to control cocoa pod borer in vitro

Nurhangga,

Nawfetrias,

Djamas

et al. 2024

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Fungal entomopathogens are suggested to be used as a bioinsecticide due to their biological persistence and ecological friendliness. Beauveria bassiana is an entomopathogenic fungus that can effectively control insect pests, including cocoa pod borer. This study aims to determine the level of effectivity of the B. bassiana (Bb) formulation product from Palu against cocoa pod borer pupae in vitro . This research used a completely randomized design (CRD) consisting of two factors: conidia concentrations and chitin treatments, which were repeated thrice. The conidia concentrations consisted of two levels, that were 106 and 107 conidia/mL. The chitin treatment included seven treatments: isolate from Kediri without formulation with chitin (Bb-K), isolate from Palu without formulation with chitin (Bb-P0), four formulations of isolates from Palu with the addition of chitin to various growth media (Bb-P1, Bb-P2, Bb-P3, and Bb-P4), and control (without Bb suspension). Bb-P0 and Bb-P2 treatments at a concentration of 107conidia/mL began to colonize CPB on the third day after the treatment application, while the other formulations started on the fifth day. However, Bb-P0 showed the lowest infection rate at the end of the observation. Contrarily, the data on the pupa-infected ratio showed that the Bb-P2 treatment was the highest compared to other treatments. It conformed to the adult emergence ratio that Bb-P2 exhibited the lowest, which means Bb-P2 has the most virulence of other formula. The result showed that PDA and PDB media-supplemented chitin was the most effective for culturing B. bassiana origin Palu before mass production.

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“…The outer cuticle of the insects is usually composed of chitin, embedded with a protein matrix. The extracellular enzymatic compounds produced by EPF can degrade these chitinous compounds and obtain nutrients from the insect’s hemolymph 24 , 25 . The fungal chitinases usually belong to class 3 chitinases and are considered an important part of the family 18 of glycosyl hydrolases 26 .…”

Section: Introductionmentioning

confidence: 99%

Chitinase of Trichoderma longibrachiatum for control of Aphis gossypii in cotton plants

Anwar,

Amin,

Khan

et al. 2023

Sci Rep

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Chitinase-producing fungi have now engrossed attention as one of the potential agents for the control of insect pests. Entomopathogenic fungi are used in different regions of the world to control economically important insects. However, the role of fungal chitinases are not well studied in their infection mechanism to insects. In this study, Chitinase of entomopathogenic fungi Trichoderma longibrachiatum was evaluated to control Aphis gossypii. For this purpose, fungal chitinase (Chit1) gene from the genomic DNA of T. longibrachiatum were isolated, amplified and characterised. Genomic analysis of the amplified Chit1 showed that this gene has homology to family 18 of glycosyl hydrolyses. Further, Chit1 was expressed in the cotton plant for transient expression through the Geminivirus-mediated gene silencing vector derived from Cotton Leaf Crumple Virus (CLCrV). Transformed cotton plants showed greater chitinase activity than control, and they were resistant against nymphs and adults of A. gossypii. About 38.75% and 21.67% mortality of both nymphs and adults, respectively, were observed by using Chit1 of T. longibrachiatum. It is concluded that T. longibrachiatum showed promising results in controlling aphids by producing fungal chitinase in cotton plants and could be used as an effective method in the future.

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Genomic Determinants of Entomopathogenic Fungi and Their Involvement in Pathogenesis

Cited by 14 publications

References 93 publications

Performance and Virulence of the Entomopathogenic Fungi Beauveria bassiana Grown in Media Derived from Biodegradable Agricultural Wastes Enriched with Cricket Powder

Performance and Virulence of the Entomopathogenic Fungi Beauveria bassiana Grown in Media Derived from Biodegradable Agricultural Wastes Enriched with Cricket Powder

The effect of chitin on the effectiveness of Beauveria bassiana formulation product to control cocoa pod borer in vitro

Chitinase of Trichoderma longibrachiatum for control of Aphis gossypii in cotton plants

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