Genomic Designing for Breeding Biotic Stress Resistant Pepper Crop

Islam, Khushbu; Kumar, Nitin; Yadava, Satish K.; Momo, John; Ramchiary, Nirala

doi:10.1007/978-3-030-97785-6_3

Cited by 5 publications

(1 citation statement)

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“…‘Dempsey’ is genetically distinguishable from other cultivars within the sweet pepper group by comparative analysis among plastome sequences [ 25 ] and displays different cellular properties in its polyethylene glycol (PEG)-mediated CRISPR/Cas9 ribonucleoprotein (RNP) delivery compared to hot pepper [ 26 , 27 ]. Various attempts to apply genome-editing tools to ‘Dempsey’ and other peppers have overcome challenges related to genetic transformation, regeneration, and the study of molecular function in pepper [ 3 , 21 , 22 , 27 – 29 ]. However, studying the molecular mechanism of shoot formation, which is essential for pepper functional genetics, precise molecular breeding, and biotechnology applications, is lacking.…”

Section: Introductionmentioning

confidence: 99%

Elucidating the callus-to-shoot-forming mechanism in Capsicum annuum ‘Dempsey’ through comparative transcriptome analyses

Han,

Park,

Won

et al. 2024

BMC Plant Biol

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Background The formation of shoots plays a pivotal role in plant organogenesis and productivity. Despite its significance, the underlying molecular mechanism of de novo regeneration has not been extensively elucidated in Capsicum annuum ‘Dempsey’, a bell pepper cultivar. To address this, we performed a comparative transcriptome analysis focusing on the differential expression in C. annuum ‘Dempsey’ shoot, callus, and leaf tissue. We further investigated phytohormone-related biological processes and their interacting genes in the C. annuum ‘Dempsey’ transcriptome based on comparative transcriptomic analysis across five species. Results We provided a comprehensive view of the gene networks regulating shoot formation on the callus, revealing a strong involvement of hypoxia responses and oxidative stress. Our comparative transcriptome analysis revealed a significant conservation in the increase of gene expression patterns related to auxin and defense mechanisms in both callus and shoot tissues. Consequently, hypoxia response and defense mechanism emerged as critical regulators in callus and shoot formation in C. annuum ‘Dempsey’. Current transcriptome data also indicated a substantial decline in gene expression linked to photosynthesis within regenerative tissues, implying a deactivation of the regulatory system governing photosynthesis in C. annuum ‘Dempsey’. Conclusion Coupled with defense mechanisms, we thus considered spatial redistribution of auxin to play a critical role in the shoot morphogenesis via primordia outgrowth. Our findings shed light on shoot formation mechanisms in C. annuum ‘Dempsey’ explants, important information for regeneration programs, and have broader implications for precise molecular breeding in recalcitrant crops.

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