Genomic damage as an independent predictor marker of mortality in hemodialysis patients

Coll, Elisabet; Stoyanova, Elitsa; Rodríguez-Ribera, Lara; Solozabal, M; Pastor, Susana; Silva, Irene; Dı́az, Juan; Ballarín, J.; Xamena, N.; Marcos, Ricard

doi:10.5414/cn107719

Cited by 11 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This agrees with the results described above, showing that genomic damage is linked to the CKD pathology itself not to the other possible alterations caused by the HD procedure. These results agree with our previous study [Coll et al, ] showing for the first time that in HD patients, the presence of high levels of genomic damage can be considered as a strong predictor of all‐cause mortality.…”

Section: Discussionsupporting

confidence: 93%

“…Specifically, patients who died during the course of the study (mean follow up of 4 years) had an average DNA damage level of 29.08 ± 2.01 ( N = 73), significantly higher than the surviving patients (12.87 ± 1.42; N = 99) Figure . The main cause of decease was cardiovascular disease [Coll et al, ]. Levels of DNA damage can therefore be viewed as a biomarker of bad prognosis in those patients.…”

Section: Resultsmentioning

confidence: 99%

“…The main cause of decease was cardiovascular Environmental and Molecular Mutagenesis. DOI 10.1002/em disease [Coll et al, 2013]. Levels of DNA damage can therefore be viewed as a biomarker of bad prognosis in those patients.…”

Section: Dna Damagementioning

confidence: 99%

See 2 more Smart Citations

Genomic damage as a biomarker of chronic kidney disease status

Corredor

Stoyanova

Rodríguez-Ribera

et al. 2014

Environ and Mol Mutagen

Self Cite

View full text Add to dashboard Cite

Patients suffering from chronic kidney disease (CKD) exhibit a high incidence of cancer and cardiovascular diseases, as well as high levels of genomic damage. To confirm the association of CKD with genomic damage we have carried out the largest study to date addressing this issue, using a total of 602 subjects (187 controls, 206 pre-dialysis CKD patients and 209 CKD patients in hemodialysis). DNA oxidative damage was measured in all individuals using the comet assay. Our results indicate that CKD patients have significantly higher levels of DNA damage than controls, but no significant differences were observed between pre-hemodialysis (pre-HD) and hemodialysis (HD) patients. When oxidative damage was measured, no differences were observed between patients and controls, although HD patients showed significantly higher levels of oxidative damage than pre-HD patients. In addition, a positive relationship was demonstrated between genomic damage and all-cause mortality. Our study confirms that genomic damage can be predictive of prognosis in CKD patients, with high levels of DNA damage indicating a poor prognosis in HD patients.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Genomic damage as a biomarker of chronic kidney disease status

Corredor

Stoyanova

Rodríguez-Ribera

et al. 2014

Environ and Mol Mutagen

Self Cite

View full text Add to dashboard Cite

show abstract

“…Table 3. Associations reported for comet assay and cell-free (cf) DNA on hemodialysis (HD) patients [96][97][98][99][100][101].…”

Section: Conclusion Remarksmentioning

confidence: 99%

DNA Damage in End-Stage Renal Disease Patients. Assessment by In Vitro Comet Assay and by Cell-Free DNA Quantification

Rocha¹,

Santos-Silva²,

Costa³

et al. 2018

Genotoxicity - A Predictable Risk to Our Actual World

View full text Add to dashboard Cite

Inflammation is a common feature in end stage renal disease (ESRD) that might contribute to increase DNA damage. ESRD patients present increased circulating cell-free DNA (cfDNA) and different types of DNA injury. The underlying inflammatory process in ESRD may be associated with increased genomic damage and cfDNA contributing to further enhance inflammation. We analyzed the degree of genomic damage in ESRD patients under hemodialysis therapy, using the comet assay and cfDNA quantification. ESRD patients presented significantly higher C-reactive protein (CRP) and cell damaged DNA. The cfDNA correlated with age and inflammatory stage. Nine out of 39 patients died during the one year follow-up period and presented significantly higher cfDNA, than those who persisted alive. At lower CRP values, the increased DNA damage is still within the cell, and at higher CRP the damaged DNA is released in to plasma. The higher degree of genomic damage in ESRD might be a consequence of inflammation and aging, and may contribute to increase cancer and cardiovascular mortality risk. Our data suggest that the comet assay is more sensitive for low-grade inflammatory conditions, while cfDNA appears as a good biomarker for more severe inflammatory conditions, and as a biomarker for the outcome of ESRD patients.

show abstract

“…This increased DNA damage does not seem to be related to the type of HD [Guido et al, ]. Moreover DNA damage has been associated with mortality in end‐stage renal disease patients treated with HD [Coll et al, ]. DNA damage has also been associated with cancer and cardiovascular disease in general population [Hagmar et al, ; Botto et al, ].…”

Section: Introductionmentioning

confidence: 99%

DNA damage in kidney transplant patients. Role of organ origin

Corredor

Rodríguez-Ribera

Coll

et al. 2017

Environ and Mol Mutagen

Self Cite

View full text Add to dashboard Cite

Chronic kidney disease (CKD) patients are characterized by elevated levels of genomic damage. This damage increases when kidney function decreases being maximum in hemodialysis patients. As kidney transplantation improves renal function, and it is related with better survival, the aim of our study was to evaluate potential changes in DNA damage levels after kidney transplantation, and comparing living donor recipients with cadaveric donor recipients. The alkaline comet assay was used to determine DNA breaks and oxidative damaged DNA; and the micronucleus assay was used to determine chromosomal breakage and/or aneuploidy. Fifty CKD patients were followed up after 6 and 12 months of their kidney transplantation. All patients increased their genomic damage levels after 6 and 12 months of renal transplantation, compared with those observed before transplantation, despite of the improvement of their metabolic functions. Donor advanced age correlated positively with higher DNA damage. Genomic damage was lower in living donor transplants with respect to cadaveric donor transplants. Our conclusion is that DNA damage increased in kidney transplantation patients, whereas their renal function improved. Higher levels of DNA damage were found in cadaveric donor transplants when compared to living donor transplants. Environ. Mol. Mutagen. 58:712-718, 2017. © 2017 Wiley Periodicals, Inc.

show abstract

Genomic damage as an independent predictor marker of mortality in hemodialysis patients

Abstract: Our study shows for the first time that, in HD patients, the presence of high levels of genomic damage is a strong predictor of all-cause mortality. This association remains significant after adjustment for relevant covariates.

Cited by 11 publications

References 0 publications

Genomic damage as a biomarker of chronic kidney disease status

Genomic damage as a biomarker of chronic kidney disease status

DNA Damage in End-Stage Renal Disease Patients. Assessment by In Vitro Comet Assay and by Cell-Free DNA Quantification

DNA damage in kidney transplant patients. Role of organ origin

Contact Info

Product

Resources

About